This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
bacteria:t3e:xopn [2020/07/03 15:44] rkoebnik |
bacteria:t3e:xopn [2020/08/09 20:27] jvicente |
||
---|---|---|---|
Line 7: | Line 7: | ||
Class: XopN\\ | Class: XopN\\ | ||
Family: XopN\\ | Family: XopN\\ | ||
- | Prototype: XopN (// | + | Prototype: XopN (// |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
- | 3D structure: unknown - similar to phosphatase 2a (pr65 / A) (Roden //et al//., 2004). | + | 3D structure: unknown - similar to phosphatase 2a (pr65/A) (Roden //et al//., 2004). |
===== Biological function ===== | ===== Biological function ===== | ||
=== How discovered? === | === How discovered? === | ||
- | XopN was identified | + | XopN was identified |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate | + | Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate |
=== Regulation === | === Regulation === | ||
- | Start codon of //xopN// was found downstream | + | Start codon of //xopN// was found downstream |
qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the // | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the // | ||
Line 28: | Line 28: | ||
* Its homolog XopN < | * Its homolog XopN < | ||
* XopN has been shown to play a role in host defence systems causing the reduction of PAMP-triggered immune responses and reduce the callose deposition in the host tissue. Moreover the deletion of // | * XopN has been shown to play a role in host defence systems causing the reduction of PAMP-triggered immune responses and reduce the callose deposition in the host tissue. Moreover the deletion of // | ||
+ | * The role of XopN in X. oryzae pv. oryzae is dependent on leaf stage (Cheong et al., 2013). | ||
+ | * XopN has been shown to be required for maximal pathogenicity of //X. axonopodis// | ||
+ | * A Δ// | ||
+ | * // | ||
+ | * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae// | ||
=== Localization === | === Localization === | ||
- | XopN was localized by confocal microscopy using fluorescent tagged fusion (yellow fluorescent protein [YFP]-XopN). [YFP]-XopN was localized throughout the plant cytoplasm and also associated with the plant plasma membrane (PM) (Kim //et al//., 2009). | + | XopN was localized by confocal microscopy using fluorescent tagged fusion (yellow fluorescent protein [YFP]-XopN). [YFP]-XopN was localized throughout the plant cytoplasm and also associated with the plant plasma membrane (PM) (Kim //et al//., 2009). Kumar et al. (2016) demonstrated that XopN is localized in the pasma membrane of //N. benthamiana//, |
=== Enzymatic function === | === Enzymatic function === | ||
- | Unknown – Kim //et al//. (2009) did not confirm | + | XopN binds TARK1, a tomato atypical receptor kinase required for PTI. Taylor |
+ | |||
+ | Three effectors (XopZ, XopN and XopV) were shown to be able to supress the peptidoglycan-triggered MAPK activation and a triple mutant of Xoo lacking these genes showed additively reduced virulence (Long et al., 2018). | ||
=== Interaction partners === | === Interaction partners === | ||
- | XopN interact with two types of proteins in tomato: Tomato Atypical Receptor-like Kinase1 (TARK1) and four Tomato Fourteen-Three-Three isoforms (TFT1, TFT3, TFT5, and TFT6) (Kim //et al//., 2009). | + | XopN interact with two types of proteins in tomato: Tomato Atypical Receptor-like Kinase1 (TARK1) and four Tomato Fourteen-Three-Three isoforms (TFT1, TFT3, TFT5, and TFT6) (Kim //et al//., 2009). XopN interacts with the tomato 14-3-3 isoform TFT1 that functions in PTI and is a XopN virulence target (Taylor //et al.//, 2012). |
+ | |||
+ | Two rice proteins, OsVOZ2 and a putative thiamine synthase (OsXNP) were identified as targets of XopN< | ||
===== Conservation ===== | ===== Conservation ===== | ||
Line 45: | Line 54: | ||
=== In xanthomonads === | === In xanthomonads === | ||
- | Yes (//e.g.//, //X//. // | + | Yes (//e.g.//, //X. axonopodis//, //X//. // |
=== In other plant pathogens/ | === In other plant pathogens/ | ||
Line 53: | Line 62: | ||
===== References ===== | ===== References ===== | ||
- | Cheong H, Kim CY, Jeon JS, Lee BM, Sun Moon J, Hwang I (2013). // | + | Cheong H, Kim CY, Jeon JS, Lee BM, Sun Moon J, Hwang I (2013). // |
- | Jiang B, He Y, Cen W, Wei H, Jiang G, Jiang W, Hang X, Feng J, Lu G, Tang D, Tang J (2008). The type III secretion effector XopXccN | + | Dubrow Z, Sunitha S, Kim JG, et al. Tomato 14-3-3 Proteins Are Required for Xv3 Disease Resistance and Interact with a Subset |
- | Kim JG, Li X, Roden JA, Taylor KW, Aakre CD, Su B, Landone S, Kirik A, Chen Y, Baranage G, Martin BG, Mudgett BM, McLane H (2009). // | + | Guzman AR, Kim JG, Taylor KW, Lanver D, Mudgett MB. Tomato Atypical Receptor Kinase1 Is Involved in the Regulation of Preinvasion Defense. //Plant Physiol//. 2020; |
+ | |||
+ | Jiang B, He Y, Cen W, Wei H, Jiang G, Jiang W, Hang X, Feng J, Lu G, Tang D, Tang J (2008). The type III secretion effector XopXccN of // | ||
+ | |||
+ | Kim JG, Li X, Roden JA, Taylor KW, Aakre CD, Su B, Landone S, Kirik A, Chen Y, Baranage G, Martin BG, Mudgett BM, McLane H (2009). // | ||
+ | |||
+ | Kumar R, Mondal KK (2013). XopN-T3SS effector modulates in planta growth of Xanthomonas axonopodis pv. punicae and cell-wall-associated immune response to induce bacterial blight in pomegranate. Physiological and Mol. Plant Pathol. 84: 36-43. DOI: 10.1016/ | ||
+ | |||
+ | Kumar R, Soni M, Mondal KK (2016). XopN-T3SS effector of // | ||
+ | |||
+ | Liao ZX, Li JY, Mo XY, et al. Type III effectors xopN and avrBS2 contribute to the virulence of Xanthomonas oryzae pv. oryzicola strain GX01. //Res Microbiol// | ||
Liu Y, Long J, Shen D, Song C (2016). // | Liu Y, Long J, Shen D, Song C (2016). // | ||
- | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during Xanthomonas infection. Proc. Natl. Acad. Sci. U.S.A. | + | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // |
+ | |||
+ | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | ||
+ | |||
+ | Mo X, Zhang L, Liu Y, Wang X, Bai J, Lu K, Zou S, Dong H, Chen L (2020). Three proteins (Hpa2, HrpF and XopN) are concomitant type III translocators in bacterial blight pathogen of rice. Frontiers in Microbiology 11: 1601. DOI=10.3389/ | ||
+ | |||
+ | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during | ||
+ | |||
+ | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // | ||
+ | |||
+ | Taylor KW, Kim JG, Su XB, Aakre CD, Roden JA, Adams CM, Mudgett MB (2012). Tomato TFT1 is required for PAMP-triggered immunity and mutations that prevent T3S effector XopN from binding to TFT1 attenuate // | ||