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bacteria:t3e:xopo [2020/06/30 18:55] rkoebnik [References] |
bacteria:t3e:xopo [2020/07/08 18:32] rkoebnik |
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Author: Harrold van den Burg\\ | Author: Harrold van den Burg\\ | ||
- | Internal reviewer: | + | Internal reviewer: |
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
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=== How discovered? === | === How discovered? === | ||
- | XopO was discovered by a random transponson insertion (Tn5) screen using a AvrBs2< | ||
+ | XopO was identified in a genetic screen, using a Tn// | ||
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | XopO fused to the Cya reporter was used to show that it is translocated into plant cells in a // | ||
+ | Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). | ||
=== Regulation === | === Regulation === | ||
- | XopO was found to be regulated by HrpG using HrpG* (Roden //et al//., 2004). //XopO// contains a PIP box sequence 31bp upstream of the -10 promoter motif (Koebnik //et al//., 2006). | ||
+ | XopO was found to be regulated by HrpG using HrpG* (Roden //et al//., 2004). //XopO// contains a PIP box sequence 31bp upstream of the -10 promoter motif (Koebnik //et al//., 2006). | ||
=== Phenotypes === | === Phenotypes === | ||
- | XopO from Xcv 85-10 inhibits cell death in //N. benthamiana// | + | |
+ | * Roden et al. did not find significant growth defects of a // | ||
+ | * XopO from //Xcv// | ||
+ | * XopO suppresses //X. euvesicatoria-// | ||
+ | * XopO failed to inhibit expression of the reporter gene // | ||
+ | * Based on whole genome sequences of //X. euvesicatoria// | ||
=== Localization === | === Localization === | ||
+ | |||
Unknown. | Unknown. | ||
=== Enzymatic function === | === Enzymatic function === | ||
+ | |||
Unknown. | Unknown. | ||
=== Interaction partners === | === Interaction partners === | ||
+ | |||
XopO was shown to interact with tomato 14-3-3- proteins (TFT) (Dubrow //et al//., 2018). | XopO was shown to interact with tomato 14-3-3- proteins (TFT) (Dubrow //et al//., 2018). | ||
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=== In xanthomonads === | === In xanthomonads === | ||
- | Yes, in some //Xanthomonads// | + | |
+ | Yes, in some xanthomonads (//e.g.//, //X. euvesicatoria//, | ||
=== In other plant pathogens/ | === In other plant pathogens/ | ||
- | Yes (HopK1 from //Pseudomonas syringae// pv. //tomato// HopPtoK, HolPtoAB); N-terminal domain of AvrRps4 from // | + | |
+ | Yes, //e.g.// // | ||
===== References ===== | ===== References ===== | ||
- | Barak JD, Vancheva T, Lefeuvre P, Jones JB, Timilsina S, Minsavage GV, Vallad GE, Koebnik R (2016) Whole-genome sequences of // | + | Barak JD, Vancheva T, Lefeuvre P, Jones JB, Timilsina S, Minsavage GV, Vallad GE, Koebnik R (2016) Whole-genome sequences of // |
- | Dubrow Z, Sunitha S, Kim JG, Aakre CD, Girija AM, Sobol G, Teper D, Chen YC, Ozbaki-Yagan N, Vance H, Sessa G, Mudgett MB (2018). Tomato 14-3-3 proteins are required for //Xv3// disease resistance and interact with a subset of // | + | Dubrow Z, Sunitha S, Kim JG, Aakre CD, Girija AM, Sobol G, Teper D, Chen YC, Ozbaki-Yagan N, Vance H, Sessa G, Mudgett MB (2018). Tomato 14-3-3 proteins are required for // |
Hajri A, Brin C, Zhao S, David P, Feng JX, Koebnik R, Szurek B, Verdier V, Boureau T, Poussier S (2012). Multilocus sequence analysis and type III effector repertoire mining provide new insights into the evolutionary history and virulence of // | Hajri A, Brin C, Zhao S, David P, Feng JX, Koebnik R, Szurek B, Verdier V, Boureau T, Poussier S (2012). Multilocus sequence analysis and type III effector repertoire mining provide new insights into the evolutionary history and virulence of // | ||
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Koebnik R, Kruger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the Xanthomonas campestris pv. vesicatoria AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https:// | Koebnik R, Kruger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the Xanthomonas campestris pv. vesicatoria AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https:// | ||
- | Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple // | + | Lang JM, Pérez-Quintero AL, Koebnik R, DuCharme E, Sarra S, Doucoure H, Keita I, Ziegle J, Jacobs JM, Oliva R, Koita O, Szurek B, Verdier V, Leach JE (2019). A pathovar of // |
+ | |||
+ | Li G, Froehlich JE, Elowsky C, Msanne J, Ostosh AC, Zhang C, Awada T, Alfano JR, (2014). Distinct // | ||
+ | |||
+ | Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple // | ||
- | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | + | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // |
- | Teper D, Sunitha S, Martin GB, Sessa G (2015). Five // | + | Teper D, Sunitha S, Martin GB, Sessa G (2015). Five // |