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bacteria:t3e:xopo

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bacteria:t3e:xopo [2020/07/03 15:15]
rkoebnik [Conservation] 		bacteria:t3e:xopo [2020/07/09 11:05]
rkoebnik [XopO]
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 Class: XopO\\ Class: XopO\\
 Family: XopO\\ Family: XopO\\
-Prototype: XopO (//Xanthomonas euvesicatoria// pv. //euvesicatoria// aka //Xanthomonas campestris// pv. //vescicatoria//; strain 85-10)\\+Prototype: XopO (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/ipg/3884105|AAV74207.1]] (220 aa)\\ RefSeq ID: [[https://www.ncbi.nlm.nih.gov/ipg/3884105|AAV74207.1]] (220 aa)\\
 3D structure: Unknown 3D structure: Unknown
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 === How discovered? === === How discovered? ===
  
-XopO was discovered by a random transponson insertion (Tn5) screen using a AvrBs2<sub>62-547</sub> reporter (readout: hypersensitive response), a construct that lacks the endogenous type-III secretion and translocation signal (Roden //et al//., 2004).+XopO was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//)// //// //strain 85-10. The XopO::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-XopO fused to the Cya reporter was used to show that it is translocated into plant cells in a //hrpF//-dependent manner (Roden //et al//., 2004).+Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004).
 === Regulation === === Regulation ===
  
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 === In xanthomonads === === In xanthomonads ===
  
-Yes, in some xanthomonads (//e.g.//, //X. euvesicatoria//, //X. oryzae//) (Lang //et al//., 2019). The //xopO// gene is a differential T3E gene between //Xoo// and //Xoc// (Hajri //et al//., 2012).+Yes, in some xanthomonads (//e.g.//, //X. euvesicatoria//, //X. oryzae//) (Lang //et al//., 2019). The //xopO//  gene is a differential T3E gene between //Xoo//  and //Xoc//  (Hajri //et al//., 2012). 
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
-Yes, //e.g.// //Pseudomonas syringae// (Li //et al//., 2014).+Yes, //e.g.//  //Pseudomonas syringae//  (Li //et al//., 2014).
  
 ===== References ===== ===== References =====

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