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bacteria:t3e:avrbs3

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bacteria:t3e:avrbs3 [2020/11/30 13:32]
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 AvrBs3, as well as other members of the TALE family, function as specific transcription factors in plant cells. These proteins bind to specific sequences in promoters and induce expression of downstream genes. The DNA-binding specificity is encoded in the order of individual 34-amino acid repeats which each recognize one DNA base. Different TALEs typically contain different repeats and accordingly bind to different DNA sequences and target different host genes. The contributions of individual TALEs to virulence can thus be quite diverse. AvrBs3, as well as other members of the TALE family, function as specific transcription factors in plant cells. These proteins bind to specific sequences in promoters and induce expression of downstream genes. The DNA-binding specificity is encoded in the order of individual 34-amino acid repeats which each recognize one DNA base. Different TALEs typically contain different repeats and accordingly bind to different DNA sequences and target different host genes. The contributions of individual TALEs to virulence can thus be quite diverse.
  
-Expression analysis using gene promoter fusion and western blot analysis demonstrated that //avrBs3// was expressed and resulted in a 122 kDa protein (1164 aa) which was detectable using a specific polyclonal antibody (Bonas //et al//., 1989). The AvrBs3 effector protein elicits two different types of responses in resistant or susceptible plants. In susceptible pepper plants (Early Cal Wonder; ECW), hypertrophy (i.e. enlargement of mesophyll cells) is triggered by AvrBs3 (Bonas //et al//., 1989; Bonas //et al//., 1991; Marois //et al//., 2002). //Agrobacterium// strains carrying a vector with //avrBs3// induced pustules (hypertrophy) 4-5 dpi in various solanaceous plants including //Nicotiana// //clevelandii//, //N.// //benthamiana//, //N.// //tabacum//, //Petunia hybrida//, //Physalis alkekengi//, //Solanum americanum// and potato (//S.// //tuberosum//), whereas //Agrobacterium// strains carrying an empty vector did not cause any changes in inoculated plants (Marois //et al//., 2002; Kay //et al//., 2007). Differential cDNA analysis from susceptible pepper plants infected with //Xcv// with or without AvrBs3 led to the discovery of //upa// (upregulated by AvrBs3) genes whose expression is induced by AvrBs3 (Marois //et al.//, 2002; Kay //et al//., 2007). These //UPA// genes all share a conserved promoter element, known as the //UPA// box (Kay //et al.//, 2007). //UPA20// acts as a master regulator of cell enlargement causing the hypertrophy symptoms associated with AvrBs3. Silencing of //upa20// decreased cell hypertrophy in infected plants while the expression of //UPA20// led to hypertrophy in uninfected plants (Kay //et al//., 2007).+Expression analysis using gene promoter fusion and western blot analysis demonstrated that //avrBs3// was expressed and resulted in a 122 kDa protein (1164 aa) which was detectable using a specific polyclonal antibody (Bonas //et al//., 1989). The AvrBs3 effector protein elicits two different types of responses in resistant or susceptible plants. In susceptible pepper plants (Early Cal Wonder; ECW), hypertrophy (i.e. enlargement of mesophyll cells) is triggered by AvrBs3 (Bonas //et al//., 1989; Bonas //et al//., 1991; Marois //et al//., 2002). //Agrobacterium// strains carrying a vector with //avrBs3// induced pustules (hypertrophy) 4-5 dpi in various solanaceous plants including //Nicotiana// //clevelandii//, //N.// //benthamiana//, //N.// //tabacum//, //Petunia hybrida//, //Physalis alkekengi//, //Solanum americanum// and potato (//S.// //tuberosum//), whereas //Agrobacterium// strains carrying an empty vector did not cause any changes in inoculated plants (Marois //et al//., 2002; Kay //et al//., 2007). Differential cDNA analysis from susceptible pepper plants infected with //Xcv// with or without AvrBs3 led to the discovery of //upa// (upregulated by AvrBs3) genes whose expression is induced by AvrBs3 (Marois //et al.//, 2002; Kay //et al//., 2007). These //UPA// genes all share a conserved promoter element, known as the //UPA// box (Kay //et al.//, 2007). //UPA20// acts as a master regulator of cell enlargement causing the hypertrophy symptoms associated with AvrBs3. Silencing of //UPA20// decreased cell hypertrophy in infected plants while the expression of //UPA20// led to hypertrophy in uninfected plants (Kay //et al//., 2007).
  
 In resistant pepper plants, the promoter of //Bs3// contains a //UPA// box that is bound by AvrBs3 resulting in the transcription of the gene //Bs3//. //Bs3// encodes a protein that is homologous to flavine-dependent mono-oxygenases (Römer //et al//., 2007) and its expression causes rapid cell death thus preventing the spread of the pathogen (Bonas //et al//., 1989; Bonas //et al//., 1991). In resistant pepper plants, the promoter of //Bs3// contains a //UPA// box that is bound by AvrBs3 resulting in the transcription of the gene //Bs3//. //Bs3// encodes a protein that is homologous to flavine-dependent mono-oxygenases (Römer //et al//., 2007) and its expression causes rapid cell death thus preventing the spread of the pathogen (Bonas //et al//., 1989; Bonas //et al//., 1991).
bacteria/t3e/avrbs3.1606739521.txt.gz · Last modified: 2020/11/30 13:32 by st

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