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--- bacteria:t3e:xopaw [2020/06/23 17:23]
monika
+++ bacteria:t3e:xopaw [2022/11/04 18:20]
rkoebnik [Biological function]
@@ Line 1: / Line 1: @@
 ====== XopAW ======
-<font inherit/Times New Roman,Times,serif;;inherit;;inherit>Author: Yael Helman\\
+Author: Yael Helman\\
-Internal reviewer: Monika Kałużna\\
+Internal reviewer: [[https://www.researchgate.net/profile/Monika_Kaluzna|Monika Kałużna]]\\
-Expert reviewer:</font>FIXME
+Expert reviewer: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]
-[[https://www.ncbi.nlm.nih.gov/protein/CAJ24824.1|Class: XopAW\\
+Class: XopAW\\
 Family: XopAW\\
-Prototype: XopAW (XCV3093; //Xanthomonas euvesicatoria// pv. //euvesicatoria// aka //Xanthomonas campestris// pv. //vescicatoria//; strain 85-10)\\
+Prototype: XCV3093 (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\
-RefSeq ID: CAJ24824.1]] (221 aa)\\
+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/CAJ24824.1|CAJ24824.1]] (221 aa)\\
 D structure: Unknown
-=====  Biological function =====
+===== Biological function =====
-=== <font inherit/Times New Roman,Times,serif;;inherit;;inherit>How discovered?</font>===
+=== How discovered? ===
- XopAW (XCV3093 in //X. euvesicatoria// 85-10 (was discovered using a machine-learning approach (Teper //et al//., 2016).
+XopAW (XCV3093 in //X. euvesicatoria// pv. //euvesicatoria// 85-10; was discovered using a machine-learning approach; Teper //et al//., 2016).
+=== (Experimental) evidence for being a T3E ===
-=== <font inherit/Times New Roman,Times,serif;;inherit;;inherit>(Experimental) evidence for being a T3E</font>===
+XopAW<sub>Xcv</sub> fused to the AvrBs2 reporter without type 3 secretion signal was shown to trigger a hypersensitive response in ECW20R pepper leaves (carrying the //B////s2// resistance gene) in an //hrpF//-dependent manner (Teper //et al//., 2016).
+=== Regulation ===
- XopAW fused to the AvrBs2 reporter, was shown to translocate into plant cells in an //hrpF//-dependent manner (Teper //et al//., 2016).
+Unknown.
-=== <font inherit/Times New Roman,Times,serif;;inherit;;inherit>Regulation</font>===
+=== Phenotypes ===
- Unknown.
+A //Xanthomonas euvesicatoria// 85-10 mutant defective in //xopAW// did not exhibit reduced virulence symptoms when inoculated on leaves of susceptible pepper plants, relative to wild-type 85-10 (Teper //et al//., 2016). Additionally, expression in //Arabidopsis// mesophyll protoplasts did not display any significant effect on suppression of the PTI-associated responses induced by the bacterial peptide flg22 (Popov //et al//., 2018).
+=== Localization ===
-=== <font inherit/Times New Roman,Times,serif;;inherit;;inherit>Phenotypes</font>===
+Unknown.
- A //Xanthomonas euvesicatoria// 85-10 mutant defective in //xopAW// did not exhibit reduced virulence symptoms when inoculated on leaves of susceptible pepper plants, relative to wild-type 85-10 (Teper //et al//., 2016). Additionally, expression in //Arabidopsis// mesophyll protoplasts did not lead to the suppression of the PTI-associated responses induced by the bacterial peptide flg22 (Popov //et al//., 2018).
+=== Enzymatic function ===
-=== <font inherit/Times New Roman,Times,serif;;inherit;;inherit>Localization</font>===
+XopAW contains a canonical EF-hand calcium-binding motif and can potentially interfere with host calcium signaling (Teper //et al//., 2016).
+=== Interaction partners ===
- Unknown.
+Unknown.
-=== <font inherit/Times New Roman,Times,serif;;inherit;;inherit>Enzymatic function</font>===
+===== Conservation =====
- XopAW contains a canonical EF-hand calcium-binding motif and can potentially interfere with host calcium signaling (Teper //et al//., 2016).
+=== In xanthomonads ===
-=== <font inherit/Times New Roman,Times,serif;;inherit;;inherit>Interaction partners</font>===
+Yes (e.g., //X. arboricola, X. axonopodis, X. citri, X//. //euvesicatoria//, //X. phaseoli//, all above 90% sequence identity; more distant homologs in //X. translucens//, //X. hyacinthi//, //X. bonasiae//, //X. sacchari//). Presence in strains without T3SS (//X. bonasiae//, //X. sacchari//) is atypical for type 3 effectors.
+=== In other plant pathogens/symbionts ===
- Unknown.
+Yes (e.g.//, Ralstonia solanacearum, Acidovorax avenae, Pseudomonas syringae, Rhizobium//) (Teper //et al.//, 2016).
+===== References =====
-=====  Conservation =====
+Popov G, Fraiture M, Brunner F, Sessa G (2018). Multiple //Xanthomonas euvesicatoria// type III effectors inhibit flg22-triggered immunity. Mol. Plant Microbe Interact. 29: 651-660. DOI: [[https://doi.org/10.1094/MPMI-07-16-0137-R|10.1094/MPMI-07-16-0137-R]]
-===  In xanthomonads ===
+Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel //Xanthomonas euvesicatoria// type III effector proteins by a machine-learning approach. Mol. Plant Pathol. 17: 398-411. DOI: [[https://doi.org/10.1111/mpp.12288|10.1111/mpp.12288]]
-<font inherit/Times New Roman,Times,serif;;inherit;;inherit>Yes (//e.g.//, //X//. //perforans//, //X//. axonopodis//, //X//. //citri//, //X//. //arboricola//, //X//. //phaseoli//, //X//. //campestris//, //X//. //sacchari//, //X//. //hyacinthi//). //</font>
+===== References =====
-<font inherit/Times New Roman,Times,serif;;inherit;;inherit>**In other plant pathogens/symbionts**</font>
+Popov G, Fraiture M, Brunner F, Sessa G (2018). Multiple //Xanthomonas euvesicatoria// type III effectors inhibit flg22-triggered immunity. Mol. Plant Microbe Interact. 29: 651-660. DOI: [[https://doi.org/10.1094/MPMI-07-16-0137-R|10.1094/MPMI-07-16-0137-R]]
-<font inherit/Times New Roman,Times,serif;;inherit;;inherit>//Yes (//e.g.//, Ralstonia solanacearum, Acidovorax avenae, Pseudomonas syringae, Rhizobium//)// //(//Teper //et al//., 2016). //</font>
+Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel //Xanthomonas euvesicatoria// type III effector proteins by a machine-learning approach. Mol. Plant Pathol. 17: 398-411. DOI: [[https://doi.org/10.1111/mpp.12288|10.1111/mpp.12288]]
-<font 18px/Times New Roman,Times,serif;;inherit;;inherit>**References**</font>
-[[https://doi.org/10.1094/MPMI-07-16-0137-R|// Popov G, Fraiture M, Brunner F, Sessa G (2018). Multiple //Xanthomonas euvesicatoria// type III effectors inhibit flg22-triggered immunity. Mol. Plant-Microbe Interact. 29(8):651-660. DOI: 10.1094/MPMI-07-16-0137-R]]. Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel //Xanthomonas euvesicatoria// type III effector proteins by a machine-learning approach. Mol. Plant Pathol. 17(3): 398-411. DOI: [[https://doi.org/10.1111/mpp.12288|10.1111/mpp.12288]]. //

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