This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision | ||
bacteria:t3e:xopj1 [2020/07/08 18:53] rkoebnik [XopJ1] |
bacteria:t3e:xopj1 [2020/09/21 18:15] (current) rkoebnik [XopJ1] |
||
---|---|---|---|
Line 3: | Line 3: | ||
Author: [[https:// | Author: [[https:// | ||
Internal reviewer: [[https:// | Internal reviewer: [[https:// | ||
- | Expert reviewer: | + | Expert reviewer: |
Class: XopJ\\ | Class: XopJ\\ | ||
Line 15: | Line 15: | ||
=== How discovered? === | === How discovered? === | ||
- | XopJ was initially discovered as a HrpG-induced gene in a cDNA-AFLP screen in // | + | XopJ was initially discovered as a HrpG-induced gene in a cDNA-AFLP screen in // |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
Line 24: | Line 24: | ||
=== Phenotypes === | === Phenotypes === | ||
- | Although a frameshift mutation of //xopJ// did not affect pathogenicity or bacterial growth in plants in early experiments (Noël //et al//., 2003), later studies showed that a //xopJ// mutant is slightly impaired in growth in pepper in late stages of the infection (Üstun //et al//., 2013). XopJ also suppresses | + | Although a frameshift mutation of //xopJ// did not affect pathogenicity or bacterial growth in plants in early experiments (Noël //et al//., 2003), later studies showed that a //xopJ// mutant is slightly impaired in growth in pepper in late stages of the infection (Üstun //et al//., 2013). XopJ also suppresses |
=== Localization === | === Localization === | ||
- | Following type III translocation, | + | XopJ carries a predicted N-myristoylation motif on a glycine residue at position two of the polypeptide. |
=== Enzymatic function === | === Enzymatic function === | ||
- | XopJ belongs to the group of YopJ-family effectors and is a member of the YopJ/AvrRxv family of SUMO peptidases and acetyltransferases. These are characterized as C55 cysteine proteases, ubiquitin-like proteases (deSUMOylation), | + | XopJ belongs to the group of YopJ-family effectors and is a member of the YopJ/AvrRxv family of SUMO peptidases and acetyltransferases. These are characterized as C55 cysteine proteases, ubiquitin-like proteases (deSUMOylation), |
=== Interaction partners === | === Interaction partners === | ||
- | 19S RP subunit RPT6 (RP ATPase 6) of the 26S proteasome (Üstün & Börnke, 2015). The interaction is dependent on the Walker A motif (ATP binding) of RPT6. | + | 19S RP subunit RPT6 (RP ATPase 6) of the 26S proteasome (Üstün & Börnke, 2015). The interaction is dependent on the Walker A motif (ATP binding) of RPT6. The interaction between the two proteins has been shown by yeast two-hybrid assays, //in vivo// and //in vitro// pull-down, as well as by bimolecular fluorescence assays //in planta// (Üstün et al., 2013). |
===== Conservation ===== | ===== Conservation ===== | ||