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bacteria:t3e:xopk [2020/07/08 19:26] rkoebnik old revision restored (2020/07/08 19:21) |
bacteria:t3e:xopk [2020/12/06 21:56] rbart_danforthcenter.org |
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Author: [[https:// | Author: [[https:// | ||
Internal reviewer: [[https:// | Internal reviewer: [[https:// | ||
- | Expert reviewer: | + | Expert reviewer: |
Class: XopK\\ | Class: XopK\\ | ||
Line 15: | Line 15: | ||
=== How discovered? === | === How discovered? === | ||
- | XopK was discovered | + | XopK was discovered |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | Mutation of a putative ubiquitin-conjugation enzyme (E2) binding site abolished XopK-induced degradation of rice somatic receptor kinase 2 (OsSERK2) and compromised XopK-dependent virulence (Qin //et al//., 2018). | + | Mutation of a putative ubiquitin-conjugation enzyme (E2) binding site abolished XopK-induced degradation of rice somatic receptor kinase 2 (OsSERK2) and compromised XopK-dependent virulence (Qin //et al//., 2018). Expression of XopK is HrpX-dependent (Furutani //et al//., 2006) and was observed to translocate using a Cya reporter system (Furutani //et al//., 2009). |
=== Regulation === | === Regulation === | ||
Line 26: | Line 26: | ||
=== Phenotypes === | === Phenotypes === | ||
- | Deletion of XopK has been shown not to affect the virulence of //X. oryzae// pv. //oryzae// PXO99A in rice IR24 plants; these differential results could be attributed to different genotypes of the rice cultivar or field conditions for plant growth (Song & Yang, 2010). | + | * Deletion of XopK has been shown not to affect the virulence of //X. oryzae// |
+ | * A ∆// | ||
+ | * XopK inhibits pathogen-associated molecular pattern-triggered immunity upstream of mitogen-activated protein kinase cascades (Qin //et al.//, 2018) | ||
- | A ∆//xopK// mutant strain of // | ||
- | |||
- | // | ||
=== Localization === | === Localization === | ||
The XopK sequence contains 54% hydrophobic residues and several predicted transmembrane domains. Thus, it is possible this protein is associated with host cell membranes following secretion (Mutka //et al//., 2016) | The XopK sequence contains 54% hydrophobic residues and several predicted transmembrane domains. Thus, it is possible this protein is associated with host cell membranes following secretion (Mutka //et al//., 2016) | ||
+ | |||
=== Enzymatic function === | === Enzymatic function === | ||
- | The protein has E3 ubiquinol ligase activity. The putative E2-binding site is highly conserved in the majority of members from different // | + | The protein has E3 ubiquinol ligase activity. The putative E2-binding site is highly conserved in the majority of members from different // |
=== Interaction partners === | === Interaction partners === | ||
XopK interacted with and directly ubiquitinated rice somatic embryogenic receptor kinase 2 (OsSERK2), resulting in its degradation (Qin //et al//., 2018) | XopK interacted with and directly ubiquitinated rice somatic embryogenic receptor kinase 2 (OsSERK2), resulting in its degradation (Qin //et al//., 2018) | ||
+ | |||
===== Conservation ===== | ===== Conservation ===== | ||
=== In xanthomonads === | === In xanthomonads === | ||
- | Yes (based on EDGAR; e.g., //X. oryzae// pvs. oryzae and oryzicola, //X. citri// pvs. citri, malvacearum, | + | Yes (based on EDGAR; e.g., //X. oryzae// |
=== In other plant pathogens/ | === In other plant pathogens/ | ||
- | Yes (e.g., // | + | Yes (e.g., // |
===== References ===== | ===== References ===== | ||
- | Furutani A, Nakayama T, Ochiai H, Kaku H, Kubo Y, Tsuge S (2006). Identification of novel HrpXo regulons preceded by two // | + | Furutani A, Nakayama T, Ochiai H, Kaku H, Kubo Y, Tsuge S (2006). Identification of novel HrpXo regulons preceded by two // |
+ | |||
+ | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // | ||
- | Liu Y, Long J, Shen D, Song C (2016). // | + | Liu Y, Long J, Shen D, Song C (2016). // |
Mutka AM, Fentress SJ, Sher SW, Berry JC, Pretz C, Nusinow DA, Bart R (2016). Quantitative, | Mutka AM, Fentress SJ, Sher SW, Berry JC, Pretz C, Nusinow DA, Bart R (2016). Quantitative, | ||
- | Qin J, Zhou X, Sun L, Wang K, Yang F, Liao H, Rong W, Yin J, Chen H, Chen X, Zhang J (2018). The // | + | Qin J, Zhou X, Sun L, Wang K, Yang F, Liao H, Rong W, Yin J, Chen H, Chen X, Zhang J (2018). The // |
- | Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // | + | Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // |
- | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< | + | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< |