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bacteria:t3e:xopq [2020/07/06 13:16] rkoebnik [Biological function] |
bacteria:t3e:xopq [2021/01/05 17:25] (current) rkoebnik [References] |
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Class: XopQ\\ | Class: XopQ\\ | ||
Family: XopQ\\ | Family: XopQ\\ | ||
- | Prototype: XCV4438: Xanthomonas outer protein Q from // | + | Prototype: XCV4438 |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
3D structure: [[https:// | 3D structure: [[https:// | ||
+ | |||
===== Biological function ===== | ===== Biological function ===== | ||
=== How discovered? === | === How discovered? === | ||
- | XopQ was identified in a genetic screen, using a Tn// | + | XopQ was identified in a genetic screen, using a Tn// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
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* Compatibility studies with //X. euvesicatoria// | * Compatibility studies with //X. euvesicatoria// | ||
* The avirulence activity of XopQ derivatives did not correlate with macroscopically visible plant reactions upon transient expression in //N. benthamiana// | * The avirulence activity of XopQ derivatives did not correlate with macroscopically visible plant reactions upon transient expression in //N. benthamiana// | ||
+ | * Transient co-expression of XopQ::GFP and XopS::GFP in //N. benthamiana// | ||
* XopQ suppressed cell death reactions in //N. benthamiana// | * XopQ suppressed cell death reactions in //N. benthamiana// | ||
- | * XopQ-mediated cell death suppression in //N. benthamiana// | + | * XopQ-mediated cell death suppression in //N. benthamiana// |
* A Δ// | * A Δ// | ||
* A reverse genetics screen identified Recognition of XopQ 1 (Roq1), a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, which mediates XopQ recognition in //N. benthamiana// | * A reverse genetics screen identified Recognition of XopQ 1 (Roq1), a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, which mediates XopQ recognition in //N. benthamiana// | ||
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* Roq1 was found to confer immunity to // | * Roq1 was found to confer immunity to // | ||
* Strong resistance to // | * Strong resistance to // | ||
+ | * Agrobacterium-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses. These immune responses were not observed when either protein was individually expressed in rice cells. XopQ-XopX induced rice immune responses were not observed with a XopX mutant that is defective in 14-3-3 binding (Deb //et al.//, 2020). | ||
+ | * A screen for // | ||
=== Localization === | === Localization === | ||
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Using protein-protein interaction studies in yeast and in planta, XopQ< | Using protein-protein interaction studies in yeast and in planta, XopQ< | ||
+ | |||
+ | Bimolecular fluorescence complementation assays upon transient expression in //N. benthamiana// | ||
Roq1, a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, was found to co-immunoprecipitate with XopQ, suggesting a physical association between the two proteins (Schultink //et al.//, 2017). | Roq1, a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, was found to co-immunoprecipitate with XopQ, suggesting a physical association between the two proteins (Schultink //et al.//, 2017). | ||
- | XopQ< | + | XopQ< |
+ | |||
+ | Yeast two-hybrid, bimolecular fluorescence complementation (BiFC) and co-IP assays indicated that XopQ and XopX interact with each other (Deb et al., 2020). | ||
===== Conservation ===== | ===== Conservation ===== | ||
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Büttner D, Bonas U (2010). Regulation and secretion of // | Büttner D, Bonas U (2010). Regulation and secretion of // | ||
+ | |||
+ | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // | ||
Deb S, Gupta MK, Patel HK, Sonti RV (2019). // | Deb S, Gupta MK, Patel HK, Sonti RV (2019). // |