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--- bacteria:t3e:xopr [2020/06/23 12:21]
jfpothier
+++ bacteria:t3e:xopr [2020/07/09 12:11] (current)
rkoebnik [XopR]
@@ Line 1: / Line 1: @@
 ====== XopR ======
-Author: Fernando Tavares\\
+Author: [[https://www.researchgate.net/profile/Fernando_Tavares|Fernando Tavares]]\\
-Reviewer: FIXME \\
+Reviewer: [[https://www.researchgate.net/profile/Amandine_Cunty|Amandine Cunty]]\\
 Expert reviewer: FIXME
 Class: XopR\\
 Family: XopR\\
-Prototype: XopR (//Xanthomonas oryzae// pv. //oryzicola// strain BLS256)\\
+Prototype: XOO4134 (//Xanthomonas oryzae// pv. //oryzae//; strain MAFF 311018)\\
-RefSeq ID: XopR [[https://www.ncbi.nlm.nih.gov/protein/WP_014505297.1|WP_014505297.1]] (437 aa) 3D structure: Unknown
+RefSeq ID: XopR [[https://www.ncbi.nlm.nih.gov/protein/WP_014505297.1|WP_014505297.1]] (437 aa)\\
+D structure: Unknown
 ===== Biological function =====
@@ Line 21: / Line 22: @@
 Functional studies using //hrp//-inducing and non-//hrp//-inducing media and reverse-transcriptase PCR in wild type and Xoo ∆//hrpX// mutants showed that the expression of //xopR// is //hrpX// dependent (Verma //et al//., 2019). These results are indirectly supported by previous findings showing that //X. oryza// pv. //oryza// (Xoo) deficient mutants for //xrvB//, a gene coding for a repressor of //hrp// gene expression, leads to an increase of XopR into plant cells (Kametani-Ikawa //et al//., 2011).
+qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>  , but this did not apply to //xopR// (Liu //et al.//, 2016).
 === Phenotypes ===
-In the last few years a comprehensive body of experimental evidence has been gathered supporting a multiple action of XopR in hampering host plant defenses, namely by fostering bacterial growth //in planta//, and suppressing pathogen-associated molecular patterns (PAMP) triggered host plant immunity (PTI) (Akimoto-Tomiyama //et al//., 2012; Wang //et al//., 2016; Medina //et al//., 2018; Verma //et al//., 2018; Verma //et al//., 2019). In fact, early studies suggested that XopR suppress PAMP-triggered stomatal closure in transgenic //Arabidopsis// expressing XopR (Wang //et al//., 2016). More recently, when compared with a Xoo wild type strain, //xopR// deficient mutants (Xoo ∆x//opR//) infiltrated in rice leaves led to an increase of callose deposits, and a significant higher production of reactive oxygen species (ROS), namely of hydrogen peroxide (H<sub>2</sub> O<sub>2</sub>) and superoxide anion (O<sub>2</sub> <sup>-</sup> ), known as the main components of the plant oxidative burst. Furthermore, reverse transcriptase expression analyses of eight rice genes linked to plant disease resistance (//BRI1//, //GST1//, //PR2//, //PR5//, //RAC1//, //SERK1//, //WRKY29// and //WRKY71//) were shown to be up-regulated in rice leaves inoculated with Xoo ∆x//opR// (Verma //et al//., 2018; Verma //et al//., 2019). To further support these findings, complementation of Xoo ∆x//opR// with //xopR// was able to restore the disease phenotype of the wild type Xoo strain (Verma //et al//., 2018; Verma //et al//., 2019).
+In the last few years a comprehensive body of experimental evidence has been gathered supporting a multiple action of XopR in hampering host plant defenses, namely by fostering bacterial growth //in planta//, and suppressing pathogen-associated molecular patterns (PAMP) triggered host plant immunity (PTI) (Akimoto-Tomiyama //et al//., 2012; Wang //et al//., 2016; Medina //et al//., 2018; Verma //et al//., 2018; Verma //et al//., 2019). In fact, early studies suggested that XopR suppress PAMP-triggered stomatal closure in transgenic //Arabidopsis// expressing XopR (Wang //et al//., 2016). More recently, when compared with a Xoo wild type strain, //xopR// deficient mutants (Xoo ∆x//opR//) infiltrated in rice leaves led to an increase of callose deposits, and a significant higher production of reactive oxygen species (ROS), namely of hydrogen peroxide (H<sub>2</sub> O<sub>2</sub>) and superoxide anion (O<sub>2</sub> <sup>-</sup>  ), known as the main components of the plant oxidative burst (reference FIXME ). Furthermore, reverse transcriptase expression analyses of eight rice genes linked to plant disease resistance (//BRI1//, //GST1//, //PR2//, //PR5//, //RAC1//, //SERK1//, //WRKY29// and //WRKY71//) were shown to be up-regulated in rice leaves inoculated with Xoo ∆x//opR// (Verma //et al//., 2018; Verma //et al//., 2019). To further support these findings, complementation of Xoo ∆x//opR// with //xopR// was able to restore the disease phenotype of the wild type Xoo strain (Verma //et al//., 2018; Verma //et al//., 2019).
 === Localization ===
@@ Line 33: / Line 36: @@
 === Interaction partners ===
-Co-immunoprecipitation assays indicate that XopR associates with various receptor-like cytoplasmic kinases (RLCKs), including BIK1 known to be involved in pathogen-associated molecular patterns (PAMP) to triggered stomatal closure (Wang //et al//., 2016). //In vitro// kinase assays indicate that XopR is phosphorylate by BIK1 likely affecting BIK1 targets, and possibly impairing PAMP-triggered stomatal immunity (Wang //et al//., 2016).
+Co-immunoprecipitation assays indicate that XopR associates with various receptor-like cytoplasmic kinases (RLCKs), including BIK1 known to be involved in pathogen-associated molecular patterns (PAMP) to triggered stomatal closure (Wang //et al//., 2016). //In vitro// kinase assays indicate that XopR is phosphorylated by BIK1 likely affecting BIK1 targets, and possibly impairing PAMP-triggered stomatal immunity (Wang //et al//., 2016).
 ===== Conservation =====
 === In xanthomonads ===
-Yes (e.g. //X. arboricola, X. axonopodis//, //X. campestris//, //X. citri//, //X. gardneri//, //X. oryzae//, //X. phaseoli//, //X. populi, X. vasicola//, inferred from a BlastP search for a query coverage higher than 90% and an identity percentage over 35%).
+Yes (e.g. //X. arboricola, X. axonopodis//, //X. campestris//, //X. citri//, //X. gardneri//, //X. oryzae//, //X. phaseoli//, //X. populi//, //X. vasicola//, //X. bromi//, //X. cucurbitae// inferred from a BlastP search for a query coverage higher than 90% and a percent identity over 35%).
 === In other plant pathogens/symbionts ===
@@ Line 45: / Line 48: @@
 ===== References =====
-Akimoto-Tomiyama C //et al//. (2012). XopR, a Type III Effector Secreted by //Xanthomonas oryzae// pv. //oryzae//, Suppresses Microbe-Associated Molecular Pattern-Triggered Immunity in //Arabidopsis thaliana//. Mol. Plant-Microbe Interact. 25: 505-514. DOI: [[https://doi.org/10.1094/mpmi-06-11-0167|10.1094/mpmi-06-11-0167]].
+Akimoto-Tomiyama C, Furutani A, Tsuge S, Washington EJ, Nishizawa Y, Minami E, Ochiai H (2012). XopR, a type III effector secreted by //Xanthomonas oryzae// pv. //oryzae//, suppresses microbe-associated molecular pattern-triggered immunity in //Arabidopsis thaliana//. Mol. Plant Microbe Interact. 25: 505-514. DOI: [[https://doi.org/10.1094/mpmi-06-11-0167|10.1094/mpmi-06-11-0167]]
+Furutani A, Nakayama T, Ochiai H, Kaku H, Kubo Y, Tsuge S (2006). Identification of novel HrpXo regulons preceded by two //cis//-acting elements, a plant-inducible promoter box and a −10 box-like sequence, from the genome database of //Xanthomonas oryzae// pv. //oryzae//. FEMS Microbiol. Lett. 259: 133-141. DOI: [[https://doi.org/10.1111/j.1574-6968.2006.00265.x|10.1111/j.1574-6968.2006.00265.x]]
+Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in //Xanthomonas oryzae// pv. //oryzae//. Mol. Plant Microbe Interact. 22: 96-106. DOI: [[https://doi.org/10.1094/mpmi-22-1-0096|10.1094/mpmi-22-1-0096]]
-Furutani A //et al//. (2006). Identification of novel HrpXo regulons preceded by two cis-acting elements, a plant-inducible promoter box and a −10 box-like sequence, from the genome database of //Xanthomonas oryzae// pv. //oryzae//. FEMS Microbiol. Lett. 259: 133-141. DOI: [[https://doi.org/10.1111/j.1574-6968.2006.00265.x|10.1111/j.1574-6968.2006.00265.x]].
+Kametani-Ikawa Y, Tsuge S, Furutani A, Ochiai H (2011). An H-NS-like protein involved in the negative regulation of //hrp// genes in //Xanthomonas oryzae// pv. //oryzae//. FEMS Microbiol. Lett. 319: 58-64. DOI: [[https://doi.org/10.1111/j.1574-6968.2011.02266.x|10.1111/j.1574-6968.2011.02266.x]]
-Furutani A //et al//. (2009). Identification of Novel Type III Secretion Effectors in //Xanthomonas oryzae// pv. //oryzae//. Mol. Plant-Microbe Interact. 22: 96-106. DOI: [[https://doi.org/10.1094/mpmi-22-1-0096|10.1094/mpmi-22-1-0096]].
+Liu Y, Long J, Shen D, Song C (2016). //Xanthomonas oryzae// pv. //oryzae// requires H-NS-family protein XrvC to regulate virulence during rice infection. FEMS Microbiol. Lett. 363: fnw067. DOI: [[https://doi.org/10.1093/femsle/fnw067|10.1093/femsle/fnw067]]
-Kametani-Ikawa Y //et al//. (2011). An H-NS-like protein involved in the negative regulation of //hrp// genes in //Xanthomonas oryzae// pv. //oryzae//. FEMS Microbiol. Lett. 319: 58-64. DOI: [[https://doi.org/10.1111/j.1574-6968.2011.02266.x|10.1111/j.1574-6968.2011.02266.x]].
+Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from //Xanthomonas axonopodis// pv. //manihotis// in virulence and suppression of plant immunity. Mol. Plant Pathol. 19: 593-606. DOI: [[https://doi.org/10.1111/mpp.12545|10.1111/mpp.12545]]
-Medina CA //et al//. (2018). The role of type III effectors from //Xanthomonas axonopodis// pv. //manihotis// in virulence and suppression of plant immunity. Mol. Plant Pathol. 19: 593-606. DOI: [[https://doi.org/10.1111/mpp.12545|10.1111/mpp.12545]].
+Verma G, Sharma M, Mondal KK (2018). XopR TTSS-effector regulates //in planta// growth, virulence of Indian strain of //Xanthomonas oryzae// pv. //oryzae// via suppressing reactive oxygen species production and cell wall-associated rice immune responses during blight induction. Funct. Plant Biol. 45: 561-574. DOI: [[https://doi.org/10.1071/FP17147|10.1071/FP17147]]
-Verma G, Sharma M, Mondal KK (2018). XopR TTSS-effector regulates //in planta// growth, virulence of Indian strain of //Xanthomonas oryzae// pv. //oryzae// via suppressing reactive oxygen species production and cell wall-associated rice immune responses during blight induction. Functional Plant Biol. 45: 561-574. DOI: [[https://doi.org/10.1071/FP17147|10.1071/FP17147]].
+Verma G, Mondal KK, Kulshreshtha A, Sharma M (2019). XopR T3SS-effector of //Xanthomonas oryzae// pv. //oryzae// suppresses cell death-mediated plant defense response during bacterial blight development in rice. 3 Biotech. 9: 272. DOI: [[https://doi.org/10.1007/s13205-019-1802-9|10.1007/s13205-019-1802-9]]
-Verma G //et al//. (2019). XopR T3SS-effector of //Xanthomonas oryzae// pv. //oryzae// suppresses cell death-mediated plant defense response during bacterial blight development in rice. Biotech. 9: 272. DOI: [[https://doi.org/10.1007/s13205-019-1802-9|10.1007/s13205-019-1802-9]].
+Wang S, Sun J, Fan F, Tan Z, Zou Y, Lu D (2016). A //Xanthomonas oryzae// pv. //oryzae// effector, XopR, associates with receptor-like cytoplasmic kinases and suppresses PAMP-triggered stomatal closure. Sci. China Life Sci. 59: 897-905. DOI: [[https://doi.org/10.1007/s11427-016-5106-6|10.1007/s11427-016-5106-6]]
-Wang S //et al//. (2016). A //Xanthomonas oryzae// pv. //oryzae// effector, XopR, associates with receptor-like cytoplasmic kinases and suppresses PAMP-triggered stomatal closure. Science China Life Sciences, 2016. 59: 897-905. DOI: [[https://doi.org/10.1007/s11427-016-5106-6|10.1007/s11427-016-5106-6]].
+White FF, Potnis N, Jones JB, Koebnik R (2009). The type III effectors of //Xanthomonas//. Mol. Plant Pathol. 10: 749-766. DOI: [[https://doi.org/10.1111/j.1364-3703.2009.00590.x|10.1111/j.1364-3703.2009.00590.x]]
-White FF //et al//. (2009). The type III effectors of //Xanthomonas//. Mol. Plant Pathol. 10: 749-766. DOI: [[https://doi.org/10.1111/j.1364-3703.2009.00590.x|10.1111/j.1364-3703.2009.00590.x]].
+Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]] FIXME Information needs to be added to the profile.

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