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bacteria:t3e:xopz [2020/07/09 10:36] rkoebnik [References] |
bacteria:t3e:xopz [2020/08/02 23:04] (current) jfpothier |
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Author: Marlène Lachaux\\ | Author: Marlène Lachaux\\ | ||
- | Internal reviewer: | + | Internal reviewer: |
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
Class: XopZ\\ | Class: XopZ\\ | ||
Family: XopZ\\ | Family: XopZ\\ | ||
- | Prototype: XopZ (// | + | Prototype: XopZ (// |
- | RefSeq ID: 1,414-amino-acid | + | RefSeq ID: [[https:// |
+ | 3D structure: | ||
===== Biological function ===== | ===== Biological function ===== | ||
=== How discovered? === | === How discovered? === | ||
- | In 2009, generation of PXO99 mutants for 18 non-TAL | + | The first mention of XopZ as an homolog of HopAS1 in// Xanthomonas oryzae// MAFF311018 was made by Furutani //et al//. (2009). Indeed, the locustag XOO2402 ([[https:// |
+ | |||
+ | In 2009, the generation of mutants for 18 non-TAL | ||
+ | |||
+ | XopZ2 was described in Potnis | ||
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | With a PIP box upstream of the predicted translation start site //XopZ< | + | The secretion of XopZ //in planta// was shown using a //B. pertussis// Cya translocation reporter assay (Furutani //et al//., 2009). |
=== Regulation === | === Regulation === | ||
- | The //XopZ// gene was shown to be expressed in a // | + | The //xopZ// gene was shown to be expressed in a // |
qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopZ//, were significantly reduced in the // | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopZ//, were significantly reduced in the // | ||
=== Phenotypes === | === Phenotypes === | ||
- | PXO99< | + | PXO99< |
+ | |||
+ | A role of XopZ in full virulence was also clearly shown in // | ||
=== Localization === | === Localization === | ||
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=== Enzymatic function === | === Enzymatic function === | ||
- | XopZ< | + | XopZ< |
=== Interaction partners === | === Interaction partners === | ||
XopZ interacts with a putative host E3 ubiquitin ligase protein PBP (s-ribonuclease) //in vitro// and //in vivo//. Regions containing 193 aa - 225 aa of PBP is required for interacting with XopZ. PBP is a negative regulator of host immune response based on the disease phenotype in PBP-knockout rice plants. C1A directly interacts and strongly degrades PBP through its cysteine protease activity, leading to a homeostatic state of PBP in plant cells (Zhou //et al//., 2015). | XopZ interacts with a putative host E3 ubiquitin ligase protein PBP (s-ribonuclease) //in vitro// and //in vivo//. Regions containing 193 aa - 225 aa of PBP is required for interacting with XopZ. PBP is a negative regulator of host immune response based on the disease phenotype in PBP-knockout rice plants. C1A directly interacts and strongly degrades PBP through its cysteine protease activity, leading to a homeostatic state of PBP in plant cells (Zhou //et al//., 2015). | ||
- | |||
===== Conservation ===== | ===== Conservation ===== | ||
=== In xanthomonads === | === In xanthomonads === | ||
- | Yes, is found to be conserved in all // | + | Yes, found to be conserved in all // |
=== In other plant pathogens/ | === In other plant pathogens/ | ||
- | Related genes are also found in several // | + | Related genes are also found in several // |
===== References ===== | ===== References ===== | ||
- | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // | + | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // |
Liu Y, Long J, Shen D, Song C (2016). // | Liu Y, Long J, Shen D, Song C (2016). // | ||
- | Potnis N, Krasileva K, Chow V, Almeida NF, Patil PB, Ryan RP, Sharlach M, Behlau F, Dow JM, Momol M, White FF, Preston JF, Vinatzer BA, Koebnik R, Setubal JC, Norman DJ, Staskawicz BJ, Jones JB (2011). Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper. BMC Genomics 12: 146. DOI: [[https:// | + | Lindeberg M, Stavrinides J, Chang JH, Alfano JR, Collmer A, Dangl JL, Greenberg JT, Mansfield JW, Guttman DS (2005). Proposed guidelines for a unified nomenclature and phylogenetic analysis of type III Hop effector proteins in the plant pathogen // |
+ | |||
+ | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // | ||
+ | |||
+ | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | ||
+ | |||
+ | Potnis N, Krasileva K, Chow V, Almeida NF, Patil PB, Ryan RP, Sharlach M, Behlau F, Dow JM, Momol M, White FF, Preston JF, Vinatzer BA, Koebnik R, Setubal JC, Norman DJ, Staskawicz BJ, Jones JB (2011). Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper. BMC Genomics 12: 146. DOI: [[https:// | ||
Ryan RP, Koebnik R, Szurek B, Boureau T, Bernal A, Bogdanove A, Dow JM (2009). Passing GO (gene ontology) in plant pathogen biology: a report from the // | Ryan RP, Koebnik R, Szurek B, Boureau T, Bernal A, Bogdanove A, Dow JM (2009). Passing GO (gene ontology) in plant pathogen biology: a report from the // | ||
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Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ < | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ < | ||
- | |||
- | Zhou H, Yang B (2018). Non-TAL effectors from // | ||
Zhou J (2015). Host target genes of the // | Zhou J (2015). Host target genes of the // | ||