Author: Dorota Tekielska
Internal reviewer: Jens Boch
Expert reviewer:

NA

Capsicum annuum PI163192 (Cook & Guevara, 1984).

Identified. Bs1 was backcrossed over 7 generations into the commercial pepper cultivar Early Calwonder (ECW) to produce a near-isogenic pepper cultigen (ECW10R) for identification of race 1 and race 2 pathogenic strains (Stall et al., 2009). The three resistance genes Bs1, Bs2, and Bs3 were transferred to a single plant of ECW and designated ECW123.

NA

Bs1 is a dominant resistance which results in a fast hypersensitive response reaction upon recognition of the type III effector AvrBs1 from Xanthomonas euvesicatoria pv. euvesicatoria (aka Xanthomonas campestris pv. vesicatoria). The resistance is rapidly overcome in the field by mutation of avrBs1 in the bacteria making this resistance unsuitable for commercial breeding.

NA

Capsicum chacoense (Cook & Stall, 1963).

Bs2 was the first cloned resistance gene in pepper (Tai et al., 1999). Bs2 was backcrossed over 7 generations into the commercial pepper cultivar Early Calwonder (ECW) to produce a near-isogenic pepper cultigen (ECW20R) (Stall et al., 2009). The three resistance genes Bs1, Bs2, and Bs3 were transferred to a single plant of ECW and designated ECW123.

Markers for breeding of Bs2 in pepper have been established (Truong et al., 2011). An overview of markers for different disease resistances in pepper has been published (Barka & Lee, 2020).

The dominant Bs2 resistance gene encodes an NB-LRR protein which interacts with the AvrBs2 protein from Xanthomonas euvesicatoria pv. euvesicatoria (aka Xanthomonas campestris pv. vesicatoria)and causes a hypersensitive response (Tai et al., 1999). Expression of Bs2 in other solanaceous plants, but not nonsolanaceous plants, also triggers hypersensitive response to AvrBs2. Bs2 was broadly used in resistance breeding and in 2000 nearly 100% commercial bell peppers contained the Bs2 resistance. Because pathogen strains with mutations in avrBs2 emerged, Bs2 was only effective for commercial control of bacterial spot disease for 5-6 years.

NA

Capsicum annuum PI271322 (Kim & Hartmann, 1985).

Cloned and sequenced (Roemer et al., 2007). A near-isogenic line containing the Bs3 (ECW30R) (Stall et al., 2009). The three resistance genes Bs1, Bs2, and Bs3 were transferred to a single plant of ECW and designated ECW123.

NA

Bs3 is a dominant resistance gene and not expressed during normal life of the plant. The TAL effector AvrBs3 from Xanthomonas euvesicatoria pv. euvesicatoria (aka Xanthomonas campestris pv. vesicatoria) binds to the promoter of Bs3 and causes expression which results in programmed cell death (Römer et al., 2007). Bs3 encodes an unusual flavin monooxygenase, but it is unknown whether the Bs3 protein has any enzymatic activity. The bs3 variant has a deletion in the promoter which prohibits binding of AvrBs3 (Römer et al., 2007). Instead, bs3 is triggered by binding of a variant of AvrBs3 with deletions of several repeats that cause it to recognize the modified sequence in the promoter of bs3 (Römer et al., 2007). The TALE AvrHah1 from Xanthomonas gardneri also elicits the Bs3 resistance. Commercial pepper lines containing Bs2 and Bs3 were used.

NA

Capsicum pubescens PI235047 (Stall et al., 2009).

Identified in pepper, cloned from tomato (Schornack et al., 2004).

NA

Bs4 is a dominant resistance gene. It has not been introgressed into pepper, because C. annuum can not be crossed with C. pubescens.

NA

Capsicum annuum ECW12346 (Jones et al., 2002; Vallejos et al., 2010).

Identified, mapped. bs5 has been transferred to the pepper cultivar ECW, backcrossed, and designated ECW50R.

AFLP markers are available (Vallejos et al., 2010).

bs5 is a recessive resistance which has been mapped. It is a stronger resistance than the other recessive resistance, bs6, but both resistances together have an additive effect (Vallejos et al., 2010).

NA

Capsicum annuum ECW12346 (Jones et al., 2002; Vallejos et al., 2010).

Identified. bs6 has been transferred to the pepper cultivar ECW, backcrossed, and designated ECW60R.

not available

bs6 is a recessive resistance which has not been mapped, so far. It is a weaker resistance than the other recessive resistance, bs5, but both resistances together have an additive effect (Vallejos et al., 2010).

NA

Capsicum pubescens PI235047A.

Identified.

N/A

The dominant BsT resistance gene from C. pubescens causes a hypersensitive response upon recognition of the type III effector AvrBsT from Xanthomonas euvesicatoria pv. euvesicatoria (aka Xanthomonas campestris pv. vesicatoria). Expression of avrBsT in C. annuum using Agrobacterium causes an HR and infection of papper with Xcv strains containing avrBsT on a high-copy plasmid causes a spotty hypersensitive reaction (Escolar et al., 2001) indicating that BsT is also present in C. annuum.

NA

Capsicum baccatum var. pendulum (Potnis et al., 2012).

Identified.

NA

Bs7 is a dominant resistance gene.

NA

Capsicum annuum accession PI 163192 (Sharma et al., 2022).

Mapped to a 2.3 Mb interval on the sub-telomeric region of chromosome 11 (Sharma et al., 2022). bs8 has been transferred to the pepper cultivar ECW, backcrossed, and designated ECW80R.

Markers from mapping population (Sharma et al., 2022)

bs8 is a recessive resistance which has been mapped. This resistance in ECW80R was determined to be quantitative, recessively inherited, and non-HR causing, and inhibits lesion expansion and chlorosis. Presence of the resistance in NILs decreased the in planta bacterial population by 9-fold compared to ECW (Sharma et al., 2022).

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