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bacteria:t3e:xopag [2020/07/01 09:52] rkoebnik [References] |
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- | ====== XopAG ====== | ||
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- | Author: TS group Christian Vernière\\ | ||
- | Internal reviewer: FIXME \\ | ||
- | Expert reviewer: FIXME\\ | ||
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- | Class: Xop?\\ | ||
- | Family: XopAG\\ | ||
- | Prototype: Xop? (// | ||
- | RefSeq ID: [[https:// | ||
- | 3D structure: | ||
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- | ===== Biological function ===== | ||
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- | === How discovered? === | ||
- | // | ||
- | Genes //avrGf1// and //avrGf2// were found to share low sequence similarity at the nucleotide level, except for a small region in the last 200 nucleotides of the genes, which showed a high level of identity (68%) (Gochez //et al//., 2017). The alignment of translated proteins AvrGf1 (533 amino acids) and AvrGf2 (509 amino acids) determined that AvrGf2 had a low degree of sequence identity (45% amino acid identity) with the previously identified AvrGf1. The highest sequence similarities were observed between AvrGf1 and AvrGf2 in the C-terminal portions of the effector proteins (74.5% identity at the amino acid level over 51 amino acids) (Gochez //et al//., 2017). | ||
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- | === (Experimental) evidence for being a T3E === | ||
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- | === Regulation === | ||
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- | === Phenotypes === | ||
- | All // | ||
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- | === Localization === | ||
- | AvrGf1 (Figueiredo //et al//., 2011) and AvrGf2 (Gochez //et al//., 2017) possess a N-terminal chloroplast localization signal. The signal is not shared by all members of the XopAG effector family (Gochez //et al//., 2017). Transient expression of the protein with the first 116 amino acids deleted in grapefruit leaves resulted in the elimination of the HR and a lack of accumulation of the protein in the chloroplast. | ||
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- | === Enzymatic function === | ||
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- | === Interaction partners === | ||
- | The XopAG AvrGf2 effector contains a Cyp-binding site that is essential for the elicitation of HR in citrus (Gochez //et al//., 2017). | ||
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- | ===== Conservation ===== | ||
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- | === In xanthomonads === | ||
- | Yes (e.g. //X.c.// pv. // | ||
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- | === In other plant pathogens/ | ||
- | Yes (e.g. //P.s.// pv. // | ||
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- | ===== References ===== | ||
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- | Escalon A, Javegny S, Vernière C, Noël LD, Vital K, Poussier S, Hajri A, Boureau T, Pruvost O, Arlat M, Gagnevin L (2013). Variations in type III effector repertoires, | ||
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- | Figueiredo JF, Romer P, Lahaye T, Graham JH, White FF, Jones JB (2011). // | ||
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- | Gochez AM, Shantharaj D, Potnis N, Zhou X, Minsavage GV, White FF, Wang N, Hurlbert JC, Jones JB (2017). Molecular characterization of XopAG effector AvrGf2 from // | ||
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- | Rybak M, Minsavage GV, Stall RE, Jones JB (2009). Identification of // | ||