This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision | ||
bacteria:t3e:xoph [2020/07/08 17:49] rkoebnik [XopH] |
— (current) | ||
---|---|---|---|
Line 1: | Line 1: | ||
- | ====== XopH ====== | ||
- | |||
- | Author: Isabel Rodrigues\\ | ||
- | Internal reviewer: FIXME \\ | ||
- | Expert reviewer: FIXME | ||
- | |||
- | Class: XopH\\ | ||
- | Family: XopH\\ | ||
- | Prototype: XopH (// | ||
- | |||
- | RefSeq ID: [[https:// | ||
- | 3D structure: [[https:// | ||
- | |||
- | ===== Biological function ===== | ||
- | |||
- | === How discovered? === | ||
- | |||
- | The XopH effector, also known as AvrBs1.1 (White //et al//., 2009), was discovered due to its virulent activity (Gurenn //et al//., 2006). Later, this effector began to be identified based on the coregulation with the TTS system (Gurenn //et al//., 2006), most recently began to be identified by a combination of biochemical approaches, including a new NMR-based method to discriminate inositol polyphosphate enantiomers (Blüher //et al//., 2017). | ||
- | === (Experimental) evidence for being a T3E === | ||
- | |||
- | The effector XopH, inhibited flg22-induced callose deposition //in planta// (Popov //et al//., 2016), dephosphorylates myo- inositol-hexakisphosphate (phytate, InsP6) //in vitro// and //in vivo// and enhanced disease symptoms (Blüher //et al//., 2017). | ||
- | === Regulation === | ||
- | |||
- | Unknown. | ||
- | |||
- | === Phenotypes === | ||
- | |||
- | This effector can inhibit flg22- but not ABA-inducible reporter gene activation in protoplasts act as PTI inhibitors in planta and contribute to development of disease symptoms like chlorosis (Popov //et al//., 2016). XopH liberates phosphate from the plant tissue to improve the nutritional status of the pathogen what causes the plant show obvious symptoms of phosphorus deficiency (Blüher //et al//., 2017). Transgenic //Nicotiana benthamiana// | ||
- | === Localization === | ||
- | |||
- | The effector XopH is localized in the nucleus and in the cytoplasm of the plant cell (Popov //et al//., 2016; Blüher //et al//., 2017). | ||
- | === Enzymatic function === | ||
- | |||
- | XopH is a T3E with phytate-degrading activity, //in vitro// and //in planta// (Blüher //et al//., 2017). | ||
- | === Interaction partners === | ||
- | |||
- | Unknown. | ||
- | |||
- | ===== Conservation ===== | ||
- | |||
- | === In xanthomonads === | ||
- | |||
- | Yes (//e.g. Xanthomonas campestris pv. campestris// | ||
- | === In other plant pathogens/ | ||
- | |||
- | Unknown. | ||
- | |||
- | ===== References ===== | ||
- | |||
- | Blüher D, Laha D, Thieme S, Hofer A, Eschen-Lippold L, Masch A, Balcke G, Pavlovic I, Nagel O, Schonsky A, Hinkelmann R, Wörner J, Parvin N, Greiner R, Weber S, Tissier A, Schutkowski M, Lee J, Jessen H, Schaaf G, Bonas U (2017). A 1-phytase type III effector interferes with plant hormone signaling. Nat. Commun. 8: 2159. DOI: [[http:// | ||
- | |||
- | Gurenn D, Thieme, F, Bonas U (2006). Type III effector proteins from the plant pathogen Xanthomonas and their role in the interaction with the host plant. J. Plant Physiol. 163: 233–255. DOI: [[https:// | ||
- | |||
- | Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple // | ||
- | |||
- | Potnis N, Minsavage G, Smith J K, Hurlbert J C, Norman D, Rodrigues R, Stall R E, Jones JB (2012). Avirulence proteins AvrBs7 from // | ||
- | |||
- | White FF, Potnis N, Jones JB, Koebnik R (2009). The type III effectors of // | ||
- | |||
- | ===== Further reading ===== | ||
- | |||
- | Thieme F (2006). Genombasierte Identifizierung neuer potentieller Virulenzfaktoren von // | ||
- | |||
- | Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Büttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, Lanz C, Linke B, McHardy AC, Meyer F, Mittenhuber G, Nies DH, Niesbach-Klösgen U, Patschkowski T, Rückert C, Rupp O, Schneiker S, Schuster SC, Vorhölter FJ, Weber E, Pühler A, Bonas U, Bartels D, Kaiser O (2005). Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium // | ||