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bacteria:t3e:xops [2020/08/29 23:47] sujan.timilsina |
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- | ====== XopS ====== | ||
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- | Author: [[https:// | ||
- | Internal reviewer: [[https:// | ||
- | Expert reviewer: Sujan Timilsina FIXME | ||
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- | Class: XopS\\ | ||
- | Family: XopS\\ | ||
- | Prototype: XopS (// | ||
- | RefSeq ID: -\\ | ||
- | 3D structure: Unknown | ||
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- | ===== Biological function ===== | ||
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- | === How discovered? === | ||
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- | Although no homology was found between XopS and other T3E effectors, //xopS //was putatively identified by the presence of a plant-inducer promoter (PIP) box, a lower GC content suggesting acquisition by HGT, and co-expression with other T3E genes (Schulze //et al.//, 2012). Deficient mutants and overexpression studies revealed that XopB and XopS contribute to disease symptoms and bacterial growth, and suppress plant defense gene expression (Schulze //et al.//, 2012). | ||
- | === (Experimental) evidence for being a T3E === | ||
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- | Type III secretion (T3S) assays of XopS-AvrBs3Δ2 fusion proteins indicated that XopS is secreted and translocated into the plant cells through T3S, inducing HR in Bs3 resistant pepper leaves. Further supporting the identify of XopS as a T3E, it was shown that deletion mutants (Δ// | ||
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- | For expression in //X. campestris// | ||
- | === Regulation === | ||
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- | HrpG- and HrpX-dependent co-regulation with the T3S system (Schulze //et al//., 2012). Presence of a PIP and -10 box (TTCGB‐N< | ||
- | === Phenotypes === | ||
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- | To study the contribution of the T3Es to bacterial virulence, the effector gene was individually deleted in //Xcv// strain 85‐10, and the mutant was inoculated into leaves of susceptible ECW pepper plants. In addition, induction of the HR in pepper ECW‐10R was analyzed, which is based on the recognition of the T3E AvrBs1 by the Bs1 resistance gene (Schulze //et al//., 2012). Schulze et al. 2012 studied XopS along with XopB in their study. Deletion of //xopB// or //xopS// led to significantly reduced disease symptoms, whereas the HR induction was not impaired. The mutant phenotypes of 85-10Δ// | ||
- | === Localization === | ||
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- | Unknown. | ||
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- | === Enzymatic function === | ||
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- | Unknown. | ||
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- | === Interaction partners === | ||
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- | Unknown. | ||
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- | ===== Conservation ===== | ||
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- | === In xanthomonads === | ||
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- | Yes (//e.g.//, // | ||
- | === In other plant pathogens/ | ||
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- | Unknown | ||
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- | ===== References ===== | ||
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- | Barak JD, Vancheva T, Lefeuvre P, Jones JB, Timilsina S, Minsavage GV, Vallad GE, Koebnik R (2016). Whole-genome sequences of // | ||
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- | Fonseca NP, Patané JSL, Varani AM, Felestrino EB, Caneschi WL, Sanchez AB, Cordeiro IF, Lemes CGC, Assis RAB, Garcia CCM, Belasque Jr. J, Martins Jr J, Facincani AP, Ferreira RM, Jaciani FJ, Almeida NF, Ferro JA, Moreira LM, Setubal JC. (2019). Analyses of seven new genomes of // | ||
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- | Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // | ||