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-====== XopG ======
-Author: [[https://www.researchgate.net/profile/Camila_Fernandes2|Camila Ferdandes]]\\
-Internal reviewer: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\
-Expert reviewer: FIXME
-Class: XopG\\
-Family: XopG1, XopG2, XopG3\\
-Prototype: XopG (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\
-RefSeq ID: [[http://ensemblgenomes.org/id/CAJ22929|CAJ22929]] (213 aa)\\
-D structure: [[https://swissmodel.expasy.org/repository/uniprot/Q3BW34|Q3BW34]] (Q3BW34_XANC5)
-===== Biological function =====
-=== How discovered? ===
-XopG was identified based on homology searches using tblastn analysis. Known T3E proteins from plant and animal pathogens were used as query against all contigs of the draft genomes of //X. vesicatoria//, //X. gardneri// and //X. perforans// with e-value of 10<sup>-5 </sup>  (Potnis //et al//., 2011).
-=== (Experimental) evidence for being a T3E ===
-The T3SS-dependent translocation evidence for XopG protein was confirmed using the AvrBs2 reporter gene assay (Thieme, 2006; Potnis //et al//., 2011).
-=== Regulation ===
-XopG belongs to class B and is still translocated in the absence of HpaB, being constitutively expressed (Thieme, 2006; White// et al//., 2009). XopG was identified as part of the putative HrpX regulon in //X. campestris// pv. //campestris// ATCC339138 (White //et al//., 2009; da Silva //et al//., 2002).
-=== Phenotypes ===
-A deletion of xopG not display differences in the induction of disease symptoms or hypersensibility response. XopG trigger cell death in different Solanaceae, including //Nicotiana// //tabacum// (White //et al//., 2009). XopG could be an essential pathogenicity factor in pepper (Potnis //et al//., 2011).
-=== Localization ===
-Confocal laser scanning microscopy revealed a localization of XopG::GFP exclusively to the plant cell nucleus (White //et al//., 2009).
-=== Enzymatic function ===
-XopG is a member of the HopH family from //Pseudomonas// //syringae// and encodes a protein with a putative zinc protease binding motif, typical of the zinc metalloproteases of the M27 family (similar to a clostridial toxin, botulinum toxin A from //Clostridium botulinum//) (Thieme, 2006; White //et al//., 2009; Schulze //et al//., 2012).
-=== Interaction partners ===
-Unknown.
-===== Conservation =====
-=== In xanthomonads ===
-Yes (//Xanthomonas// spp.,//X.// //euvesicatoria//, //X. vesicatoria//, //X. gardneri//, //X. campestris// pv. //campestris//, //Xanthomonas campestris// pv. //phaseoli//, //Xanthomonas translucens// pv. //translucens//, //X. translucens// pv. //graminis//, //X. citri pv. bilvae//, //X.citri pv. fuscans//, //X. oryzae// pv. //oryzae//, //X. arboricola// pv. //corylina//, //X. arboricola// pv. //pruni//).
-=== In other plant pathogens/symbionts ===
-Yes (//Ralstonia solanacearum//, //Pseudomonas// spp., //Acidovorax citrulli//).
-===== References =====
-da Silva AC, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LM, do Amaral AM, Bertolini MC, Camargo LE, Camarotte G, Cannavan F, Cardozo J, Chambergo F, Ciapina LP, Cicarelli RM, Coutinho LL, Cursino-Santos JR, El-Dorry H, Faria JB, Ferreira AJ, Ferreira RC, Ferro MI, Formighieri EF, Franco MC, Greggio CC, Gruber A, Katsuyama AM, Kishi LT, Leite RP, Lemos EG, Lemos MV, Locali EC, Machado MA, Madeira AM, Martinez-Rossi NM, Martins EC, Meidanis J, Menck CF, Miyaki CY, Moon DH, Moreira LM, Novo MT, Okura VK, Oliveira MC, Oliveira VR, Pereira HA, Rossi A, Sena JA, Silva C, de Souza RF, Spinola LA, Takita MA, Tamura RE, Teixeira EC, Tezza RI, Trindade dos Santos M, Truffi D, Tsai SM, White FF, Setubal JC, Kitajima JP (2002). Comparison of the genomes of two //Xanthomonas// pathogens with differing host specificities. Nature 417: 459-463. DOI: [[https://doi.org/10.1038/417459a|10.1038/417459a]]
-Potnis N, Krasileva K, Chow V, Almeida NF, Patil PB, Ryan RP, Sharlach M, Behlau F, Dow JM, Momol MT, White FF, Preston JF, Vinatzer BA, Koebnik R, Setubal JC, Norman DJ, Staskawicz BJ, Jones JB (2011). Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper. BMC Genomics 12: 146. DOI: [[https://doi.org/10.1186/1471-2164-12-146|10.1186/1471-2164-12-146]]
-Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from //Xanthomonas// uncovers XopB and XopS as suppressors of plant immunity. New Phytol. 195: 894-911. DOI: [[https://doi.org/10.1111/j.1469-8137.2012.04210.x|10.1111/j.1469-8137.2012.04210.x]]
-Thieme F (2006). Genombasierte Identifizierung neuer potentieller Virulenzfaktoren von //Xanthomonas// //campestris// pv. //vesicatoria//. Doctoral Thesis, Martin-Luther-Universität Halle-Wittenberg, Germany. PDF: [[http://sundoc.bibliothek.uni-halle.de/diss-online/06/06H103/prom.pdf|http://sundoc.bibliothek.uni-halle.de/diss-online/06/06H103/prom.pdf]]
-Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Büttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, Lanz C, Linke B, McHardy AC, Meyer F, Mittenhuber G, Nies DH, Niesbach-Klösgen U, Patschkowski T, Rückert C, Rupp O, Schneiker S, Schuster SC, Vorhölter FJ, Weber E, Pühler A, Bonas U, Bartels D, Kaiser O (2005). Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium //Xanthomonas campestris// pv. //vesicatoria// revealed by the complete genome sequence. J. Bacteriol. 187: 7254-7266. DOI: [[https://doi.org/10.1128/JB.187.21.7254-7266.2005|10.1128/JB.187.21.7254-7266.2005]]
-White FF, Potnis N, Jones JB, Koebnik R (2009). The type III effectors of //Xanthomonas//. Mol. Plant Pathol.10: 749-766. DOI: [[https://doi.org/10.1111/j.1364-3703.2009.00590.x|10.1111/j.1364-3703.2009.00590.x]]

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