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bacteria:t3e:xopv [2020/07/17 10:45] rkoebnik |
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- | ====== XopV ====== | ||
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- | Author: Coline Sciallano\\ | ||
- | Internal reviewer: [[https:// | ||
- | Expert reviewer: FIXME | ||
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- | Class: XopV\\ | ||
- | Family: XopV\\ | ||
- | Prototype: XOO3803 (// | ||
- | RefSeq ID: [[https:// | ||
- | 3D structure: Unknown | ||
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- | ===== Biological function ===== | ||
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- | === How discovered? === | ||
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- | XopV was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// | ||
- | === (Experimental) evidence for being a T3E === | ||
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- | XopV (XOO3803 in Furutani //et al//., 2009) has been shown to be translocated through the T3SS thanks to a XopV fusion with a calmodulin-dependant adenylate cyclase reporter in // | ||
- | === Regulation === | ||
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- | Expression of XopV has been shown to be regulated by HrpX using a construction with //xopV// and an adenylate cyclase reporter system in hrp-inducing medium. Also, no adenylate cyclase activity and no //xopV// transcript accumulation could be detected in a //hrpX// deficient strain with the same construction (Furutani //et al//., 2009). | ||
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- | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopV//, were significantly reduced in the //Xoo// Δ//xrvC// mutant compared with those in the wild-type strain PXO99< | ||
- | === Phenotypes === | ||
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- | An individual mutant for //xopV// in //Xoo // | ||
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- | // | ||
- | === Localization === | ||
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- | Unknown. | ||
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- | === Enzymatic function === | ||
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- | Unknown. | ||
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- | === Interaction partners === | ||
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- | Unknown. | ||
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- | ===== Conservation ===== | ||
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- | === In xanthomonads === | ||
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- | Yes, examples: //X. campestris//, | ||
- | === In other plant pathogens/ | ||
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- | Yes, examples: // | ||
- | ===== References ===== | ||
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- | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // | ||
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- | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Tsuge S (2009). Identification of novel type III secretion effectors in // | ||
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- | Liu Y, Long J, Shen D, Song C (2016). // | ||
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- | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // | ||
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- | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< | ||