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bacteria:t3e:xopae

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bacteria:t3e:xopae [2020/08/13 11:51]
lnoel 		bacteria:t3e:xopae [2020/08/13 12:14]
lnoel
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 Prototype: XopAE (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\ Prototype: XopAE (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011050288.1|WP_011050288.1]] (547 aa)\\ RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011050288.1|WP_011050288.1]] (547 aa)\\
-Synonyms: XopAE was also referred to as HpaF (Kim //et al.//, 2003; Sugio //et al.//, 2005). In //Xcv// strain 85-10, the gene is bipartite and the two parts were first coined //hpaF// and //hpaG// (Noël //et al.//, 2002). However, //hpaG// was also used in other Xanthomonas to designate the homolog of //hpa1// (//X. campestris//, //X. oryzae//) and //xopA// (//Xcv// 85-10) (Kim //et al.//, 2003).\\ +Synonyms: XopAE was also referred to as HpaF (Kim //et al.//, 2003; Sugio //et al.//, 2005). In //Xcv// strain 85-10, the gene is bipartite and the two parts were first coined //hpaF// and //hpaG// (Noël //et al.//, 2002). Be careful: //hpaG// was also used in other Xanthomonas to designate the homolog of //hpa1// (//X. campestris//, //X. oryzae//) and //xopA// (//Xcv// 85-10) (Kim //et al.//, 2003).\\ 
-3D structure: Most XopAE pocesses a N-terminal leucine-rich repeat (LRR) domain and a C-terminal XL box motif with an E3 ubiquitin ligase fold, based on homology modelling. Predicted fold of XopAE C terminus is similar to that of XopL (Popov //et al//., 2018).+3D structure: Most XopAE pocesses a N-terminal leucine-rich repeat (LRR) domain and a C-terminal XL box motif with an E3 ubiquitin ligase fold, based on homology modelling (Popov //et al//., 2018). Predicted fold of XopAE C terminus is similar to that of XopL (Popov //et al//., 2018).
 ===== The locus ===== ===== The locus =====
  
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 === How discovered? === === How discovered? ===
  
-XopAE was discovered by studying //hrpG//-regulated genes with the //hrp// gene cluster of //X. euvesicatoria// strain 85-10 (Noël //et al//., 2002).+XopAE was discovered by studying //hrpG//-regulated genes within the //hrp// gene cluster of //X. euvesicatoria// strain 85-10 (Noël //et al//., 2002).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-XopAE preceded by //hpaF// was fused to the AvrBs2 reporter and shown to translocate into plant cells in an //hrpF//-dependent manner (Popov //et al//., 2018).+XopAE<sub>85-10</sub> was fused to the AvrBs2 reporter and shown to translocate into plant cells in an //hrpF//-dependent manner (Popov //et al//., 2018).
 === Regulation === === Regulation ===
  
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 === Phenotypes === === Phenotypes ===
  
-XopAE<sub>85-10</sub> is a suppressor of PTI that was shown to inhibit flg22-mediated signaling in //Arabidopsis// and tomato protoplasts, downstream or in parallel to the activation of the MPK3 and MPK6 MAP kinases. Expression of XopAE<sub>85-10</sub> in plants inhibits PTI-associated callose deposition at the cell wall and enhances disease symptoms (Popov //et al//., 2016).+XopAE<sub>85-10</sub> is a suppressor of PTI that was shown to inhibit flg22-mediated signaling in //Arabidopsis// and tomato protoplasts, downstream or in parallel to the activation of the MPK3 and MPK6 MAP kinases. //Pseudomonas syringae//-mediated delivery of XopAE<sub>85-10</sub> in plants inhibits PTI-associated callose deposition at the cell wall and enhances disease symptoms in tomato (Popov //et al//., 2016).
 === Localization === === Localization ===
  
-Cytoplasm and nucleus (Popov //et al//., 2016).+XopAE<sub>85-10</sub>-YFP fusion protein localizes to the cytoplasm and nucleus of //Nicotiana benthamiana// leaf epidermal cells (Popov //et al//., 2016).
 === Enzymatic function === === Enzymatic function ===
  
-XopAE is an active E3 ubiquitin ligase //in vitro// (Popov //et al//., 2018).+XopAE<sub>85-10</sub> is an active E3 ubiquitin ligase //in vitro// (Popov //et al//., 2018).
 === Interaction partners === === Interaction partners ===
  
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 === In xanthomonads === === In xanthomonads ===
  
-Yes (//e.g.//, the //xopAE// locus is present in sequenced genomes of //X. alfalfae//, //X. euvesicatoria//, //X. oryzae//, //X. bromi//, //X. vasicola// and //X. fragariae//).+Yes (Popov //et al//., 2018). The //xopAE// locus is present in sequenced genomes of //X. alfalfae//, //X. euvesicatoria//, //X. oryzae//, //X. bromi//, //X. vasicola// and //X. fragariae// but not //X. campestris// pv. //campestris, for instance//.
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
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 ===== Further reading ===== ===== Further reading =====
- 
-Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple //Xanthomonas euvesicatoria// type III effectors inhibit flg22-triggered immunity. Mol. Plant Microbe Interact. 29: 651-660. DOI: [[https://doi.org/10.1094/MPMI-07-16-0137-R|10.1094/MPMI-07-16-0137-R]] 
  
 Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]] Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]]
  
bacteria/t3e/xopae.txt · Last modified: 2020/09/10 18:32 by rkoebnik

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