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bacteria:t3e:xope1 [2020/07/01 12:01] rkoebnik [Biological function] |
bacteria:t3e:xope1 [2020/07/08 18:51] (current) rkoebnik [XopE1] |
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====== XopE1 ====== | ====== XopE1 ====== | ||
- | Author: Jaime Cubero\\ | + | Author: |
- | Internal reviewer: Ralf Koebnik\\ | + | Internal reviewer: |
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
Class: XopE\\ | Class: XopE\\ | ||
Family: XopE1\\ | Family: XopE1\\ | ||
- | Prototype: XCV0294 (// | + | Prototype: XCV0294 (// |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
Synonym: AvrXacE1 (// | Synonym: AvrXacE1 (// | ||
- | 3D structure: | + | 3D structure: |
- | ===== Conservation | + | ===== Biological function |
- | === In xanthomonads | + | === How discovered? |
- | Yes (//e.g.//, | + | XopE1 was first identified by sequence homology searches |
- | === In other plant pathogens/ | + | === (Experimental) evidence for being a T3E === |
- | Yes (//Pseudomonas// //syringae//, //Ralstonia// //solanacearum//). | + | XopE1 fused to the AvrBs3 reporter was shown to be secreted into culture supernatants in a //hrcV-//dependent manner (Thieme |
- | ===== References ===== | + | === Regulation |
- | Adlung N, Prochaska H, Thieme S, Banik A, Blüher D, John P, Nagel O, Schulze S, Gantner J, Delker C, Stuttmann J, Bonas U (2016). Non-host resistance induced by the //Xanthomonas// effector XopQ is widespread within the genus //Nicotiana// and functionally depends on EDS1. Front. Plant Sci. 7: 1796. DOI: [[https://doi.org/10.3389/fpls.2016.01796|10.3389/fpls.2016.01796]] | + | Using RT-PCR analyses, XopE1 from //X. euvesicatoria// was found to be upregulated by HrpG and HrpX (Thieme |
- | Assis RAB, Polloni LC, Patané JSL, Thakur S, Felestrino ÉB, Diaz-Caballero J, Digiampietri LA, Goulart LR, Almeida NF, Nascimento R, Dandekar AM, Zaini PA, Setubal JC, Guttman DS, Moreira LM (2017). Identification | + | Transcriptome analysis (RNA-seq) and qRT-PCR revealed that //avrXacE1// (//xopE1//) gene expression is downregulated in a //X. citri// pv. //citri// Δ//phoP// mutant, indicating that PhoP is a positive regulator of //xopE1// expression (Wei et al., 2019). |
+ | === Phenotypes === | ||
- | da Silva AC, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LM, do Amaral AM, Bertolini MC, Camargo LE, Camarotte G, Cannavan F, Cardozo J, Chambergo F, Ciapina LP, Cicarelli RM, Coutinho LL, Cursino-Santos JR, El Dorry H, Faria JB, Ferreira AJ, Ferreira RC, Ferro MI, Formighieri EF, Franco MC, Greggio CC, Gruber A, Katsuyama AM, Kishi LT, Leite RP, Lemos EG, Lemos MV, Locali EC, Machado MA, Madeira AM, Martinez-Rossi NM, Martins EC, Meidanis J, Menck CF, Miyaki CY, Moon DH, Moreira LM, Novo MT, Okura VK, Oliveira, MC, Oliveira VR, Pereira HA, Rossi A, Sena JA, Silva C, de Souza RF, Spinola LA,Takita MA, Tamura RE, Teixeira EC, Tezza RI, Trindade dos SM, Truffi D, Tsai, SM, White FF, Setubal JC, Kitajima JP (2002). Comparison of the genomes | + | // |
+ | === Localization === | ||
- | Dubrow Z, Sunitha S, Kim JG, Aakre CD, Girija AM, Sobol G, Teper D, Chen YC, Ozbaki-Yagan N, Vance H, Sessa G, Mudgett MB (2018). Tomato 14-3-3 proteins are required for //Xv3// disease resistance and interact with a subset of //Xanthomonas euvesicatoria// effectors. Mol. Plant Microbe Interact. 31: 1301-1311. DOI: [[https:// | + | XopE1 fused to GFP reporter in a binary vector under control of the Cauliflower mosaic virus 35S promoter and transiently expressed in //Nicotiana benthamiana// leaves, using //Agrobacterium-//mediated gene transfer, allowed to observe XopE1::GFP to be confined to the periphery of the cells and being not detectable in the nucleus or in the cytoplasm. |
+ | === Enzymatic function === | ||
- | Nimchuk ZL, Fisher EJ, Desvaux D, Chang JH, Dangl JL (2007). The HopX (AvrPphE) | + | XopE1 belongs to the HopX effector |
- | + | === Interaction partners === | |
- | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression | + | |
- | + | ||
- | Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Büttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, Lanz C, Linke B, McHardy AC, Meyer F, Mittenhuber G, Nies DH, Niesbach-Klösgen U, Patschkowski T, Rückert C, Rupp O, Schneiker S, Schuster SC, Vorhölter F, Weber E, Pühler A, Bonas U, Bartels D, Kaiser O (2005). Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium | + | |
- | + | ||
- | Thieme F, Szczesny R, Urban A, Kirchner O, Hause G, Bonas U (2007). | + | |
+ | XopE1 was found to physically interact with tomato 14-3-3s (TFT) (Dubrow //et al.//, 2018). In addition, XopE1 was predicted to interact with VirK, which is secreted by the T2SS and for which a possible role in the modulation of plant immune response during the infection process was suggested (Assis //et al//., 2017). | ||
===== Conservation ===== | ===== Conservation ===== | ||
=== In xanthomonads === | === In xanthomonads === | ||
- | Yes (//e.g.//, //X. citri, X. campestris, X. phaseoli, X. alfalfa, X. euvesicatoria// | ||
+ | Yes (//e.g.//, //X. alfalfa, X. citri, X. euvesicatoria//, | ||
=== In other plant pathogens/ | === In other plant pathogens/ | ||
- | Yes (// | ||
+ | Yes (// | ||
===== References ===== | ===== References ===== | ||
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Thieme F, Szczesny R, Urban A, Kirchner O, Hause G, Bonas U (2007). New type III effectors from // | Thieme F, Szczesny R, Urban A, Kirchner O, Hause G, Bonas U (2007). New type III effectors from // | ||
+ | |||
+ | Wei C, Ding T, Chang C, Yu C, Li X, Liu Q (2019). Global regulator PhoP is necessary for motility, biofilm formation, exoenzyme production and virulence of // | ||