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--- bacteria:t3e:xope2 [2020/07/15 13:15]
rkoebnik [Biological function]
+++ bacteria:t3e:xope2 [2020/07/15 13:16]
rkoebnik [Biological function]
@@ Line 36: / Line 36: @@
 === Localization ===
-XopE2 fused to gfp in a binary vector under control of the Cauliflower mosaic virus 35S promoter expressed in //Nicotiana benthamiana//  leaves, using //Agrobacterium//-mediated gene transfer, allowed to localize XopE2::GFP confined to the periphery of the cells and not detectable in the nucleus or in the cytoplasm. Localization of the XopE2::GFP to the plasma membrane of //N. benthamiana //mesophyll cells could be confirmed by immunocytochemistry (Thieme //et al//., 2007).
+XopE2 fused to GFP in a binary vector under control of the Cauliflower mosaic virus 35S promoter expressed in //Nicotiana benthamiana//  leaves, using //Agrobacterium//-mediated gene transfer, allowed to localize XopE2::GFP confined to the periphery of the cells and not detectable in the nucleus or in the cytoplasm. Localization of the XopE2::GFP to the plasma membrane of //N. benthamiana //mesophyll cells could be confirmed by immunocytochemistry (Thieme //et al//., 2007).
 === Enzymatic function ===

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