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bacteria:t3e:xopf [2020/07/08 18:30]
rkoebnik 		bacteria:t3e:xopf [2020/09/10 18:31] (current)
rkoebnik
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 Author: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\ Author: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\
 Internal reviewer: [[https://www.researchgate.net/profile/Jaime_Cubero|Jaime Cubero]]\\ Internal reviewer: [[https://www.researchgate.net/profile/Jaime_Cubero|Jaime Cubero]]\\
-Expert reviewer: FIXME+Expert reviewer: [[https://www.researchgate.net/profile/Kalyan_Mondal|Kalyan K Mondal]]
  
 Class: XopF\\ Class: XopF\\
 Family: XopF1, XopF2, XopF3\\ Family: XopF1, XopF2, XopF3\\
-Prototype: XopF (//Xanthomonas euvesicatoria// pv. //euvesicatoria//,ex //Xanthomonas campestris //pv. //vesicatoria//; strain 85-10)\\ +Prototype: XopF (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris //pv. //vesicatoria//; strain 85-10)\\ 
-RefSeq ID: XopF1 [[https://www.ncbi.nlm.nih.gov/protein/WP_011346095.1|WP_011346095.1]] (670 aa), XopF2 [[https://www.ncbi.nlm.nih.gov/protein/56121735|AAV74205.1]] (667 aa)\\+RefSeq ID: XopF1_Xe [[https://www.ncbi.nlm.nih.gov/protein/WP_011346095.1|WP_011346095.1]] (670 aa), XopF1_Xoo [[https://www.ncbi.nlm.nih.gov/protein/589298639|WP_AHK80891]] (661 aa), XopF2 [[https://www.ncbi.nlm.nih.gov/protein/56121735|AAV74205.1]] (667 aa)\\ 
 +Synonym: Hpa4\\
 3D structure: Unknown 3D structure: Unknown
  
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 === How discovered? === === How discovered? ===
  
-XopF1 and XopF2 were identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//)// //strain 85-10. The XopF1::AvrBs2 and XopF2::AvrBs2 fusion proteins triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004).+XopF1 and XopF2 were identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//) strain 85-10. The XopF1::AvrBs2 and XopF2::AvrBs2 fusion proteins triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-Type III-dependent secretion of XopF1 and XopF2 was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004).+Type III-dependent secretion of XopF1 and XopF2 was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004, Mondal //et al.//, 2016).
  
 Fragments of the //xopF1// gene are located within the //hrp// cluster of many //Xanthomonas// spp., although a complete ORF is present only in the //Xcv// and //Xanthomonas oryzae// pv. //oryzae// (//Xoo//) //hrp// clusters (Roden //et al//., 2004). Fragments of the //xopF1// gene are located within the //hrp// cluster of many //Xanthomonas// spp., although a complete ORF is present only in the //Xcv// and //Xanthomonas oryzae// pv. //oryzae// (//Xoo//) //hrp// clusters (Roden //et al//., 2004).
  
-XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an Xcv chaperone (Roden //et al//., 2004).+XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an //Xcv// chaperone (Roden //et al//., 2004).
 === Regulation === === Regulation ===
  
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 === Phenotypes === === Phenotypes ===
  
-  * Roden et al. did not find significant growth defects of a //Xcv//  Δ//xopF1//  or Δ//xopF2//  mutant in susceptible pepper and tomato leaves (Roden et al., 2004) +  * Roden et al. did not find significant growth defects of a //Xcv//  Δ//xopF1//  or Δ//xopF2//  mutant in susceptible pepper and tomato leaves (Roden //et al.//, 2004) 
-  * To study the possible virulence function of the putative //xopF1//  operon encoding HpaD, HpaI, and XopF1 these three genes were deleted from the genome of //X. campestris//  pv. //vesicatoria//  85-10. The resultant mutant strain 85-10Δ//EF//  displayed a wild-type phenotype when infiltrated into susceptible and resistant plants. To investigate a possible functional redundancy due to homologous genes, //xopF2//  and the flanking ORF //XCV2943//  were also deleted in strain 85-10Δ//EF//. Since the resulting multiple mutant strain 85-10Δ//EF//Δ//xopF2//  also behaved like the wild type in infection tests//, xopF1//  and //xopF2//  regions did not seem to play an obvious role in the bacterial interaction with the host plant. (Büttner //et al//., 2007).+  * To study the possible virulence function of the putative //xopF1//  operon encoding HpaD, HpaI, and XopF1 these three genes were deleted from the genome of //Xcv //85-10. The resultant mutant strain 85-10Δ//EF//  displayed a wild-type phenotype when infiltrated into susceptible and resistant plants. To investigate a possible functional redundancy due to homologous genes, //xopF2//  and the flanking ORF //XCV2943//  were also deleted in strain 85-10Δ//EF//. Since the resulting multiple mutant strain 85-10Δ//EF//Δ//xopF2//  also behaved like the wild type in infection tests//, xopF1//  and //xopF2//  regions did not seem to play an obvious role in the bacterial interaction with the host plant (Büttner //et al//., 2007).
   * Later, //Xoo//  XopF1 was proven to contribute to virulence in rice, as infection with //xopF1//  mutant has shown a reduced lesion size comparing to wild type (Mondal //et al//., 2016).   * Later, //Xoo//  XopF1 was proven to contribute to virulence in rice, as infection with //xopF1//  mutant has shown a reduced lesion size comparing to wild type (Mondal //et al//., 2016).
-  * Additionally, XopF1 and XopF2 of //X. euvesicatoria//  and //Xoo//  seem to have a role in PTI suppression //in planta//  namely by inhibiting callose deposition and by suppressing the induction of PTI marker genes, overall contributing to development of symptoms (Mondal //et al//., 2016; Popov //et al//., 2016).+  * Additionally, XopF1 and XopF2 of //X. euvesicatoria//  and //Xoo//  seem to have a role in PTI suppression //in planta//, namely by inhibiting callose deposition and by suppressing the induction of PTI marker genes, overall contributing to development of symptoms (Mondal //et al//., 2016; Popov //et al//., 2016).
   * //Xoo//  XopF1 triggered an HR in non-host plants (Li //et al//., 2016).   * //Xoo//  XopF1 triggered an HR in non-host plants (Li //et al//., 2016).
 === Localization === === Localization ===
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 === In xanthomonads === === In xanthomonads ===
  
-Yes (//e.g.//, //X. arboricola, X. bromi//, //X. citri, X. oryzae//, //X. euvesicatoria//, //X. translucens//, //X. vasicola//). Since the G+C content of the //xopF1//  gene is similar to that of the //Xcv//  //hrp//  gene cluster, it may be a member of a “core” group of //Xanthomonas//  spp. effectors (Roden et al., 2004).+Yes (//e.g.//, //X. arboricola, X. bromi//, //X. citri, X. oryzae//  pv.// oryzae//, //X. euvesicatoria//, //X. translucens//, //X. vasicola//). Since the G+C content of the //xopF1//  gene is similar to that of the //Xcv//  //hrp//  gene cluster, it may be a member of a “core” group of //Xanthomonas//  spp. effectors (Roden //et al.//, 2004).
  
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
bacteria/t3e/xopf.1594225835.txt.gz · Last modified: 2020/07/08 18:30 by rkoebnik

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