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bacteria:t3e:xopf [2020/08/07 15:10] kalyanmondal [XopF] |
bacteria:t3e:xopf [2020/09/10 18:31] (current) rkoebnik |
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=== How discovered? === | === How discovered? === | ||
- | XopF1 and XopF2 were identified in a genetic screen, using a Tn// | + | XopF1 and XopF2 were identified in a genetic screen, using a Tn// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | Type III-dependent secretion of XopF1 and XopF2 was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004, Mondal et al, 2016). | + | Type III-dependent secretion of XopF1 and XopF2 was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004, Mondal |
Fragments of the //xopF1// gene are located within the //hrp// cluster of many // | Fragments of the //xopF1// gene are located within the //hrp// cluster of many // | ||
- | XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an Xcv chaperone (Roden //et al//., 2004). | + | XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an //Xcv// chaperone (Roden //et al//., 2004). |
=== Regulation === | === Regulation === | ||
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=== Phenotypes === | === Phenotypes === | ||
- | * Roden et al. did not find significant growth defects of a // | + | * Roden et al. did not find significant growth defects of a // |
- | * To study the possible virulence function of the putative // | + | * To study the possible virulence function of the putative // |
* Later, // | * Later, // | ||
* Additionally, | * Additionally, | ||
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=== In xanthomonads === | === In xanthomonads === | ||
- | Yes (//e.g.//, //X. arboricola, X. bromi//, //X. citri, X. oryzae// pv.// oryzae//, //X. euvesicatoria//, | + | Yes (//e.g.//, //X. arboricola, X. bromi//, //X. citri, X. oryzae// |
=== In other plant pathogens/ | === In other plant pathogens/ | ||