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bacteria:t3e:xopg [2020/07/08 18:37]
rkoebnik [XopG] 		bacteria:t3e:xopg [2020/12/02 22:58] (current)
jfpothier
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 Author: [[https://www.researchgate.net/profile/Camila_Fernandes2|Camila Ferdandes]]\\ Author: [[https://www.researchgate.net/profile/Camila_Fernandes2|Camila Ferdandes]]\\
 Internal reviewer: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\ Internal reviewer: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\
-Expert reviewer: FIXME+Expert reviewer: [[https://scholar.google.com/citations?user=YqEpuD0AAAAJ&hl=en|Neha Potnis]]
  
 Class: XopG\\ Class: XopG\\
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 Prototype: XopG (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\ Prototype: XopG (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\
 RefSeq ID: [[http://ensemblgenomes.org/id/CAJ22929|CAJ22929]] (213 aa)\\ RefSeq ID: [[http://ensemblgenomes.org/id/CAJ22929|CAJ22929]] (213 aa)\\
-3D structure: [[https://swissmodel.expasy.org/repository/uniprot/Q3BW34|Q3BW34]] (Q3BW34_XANC5)+3D structure: [[https://swissmodel.expasy.org/repository/uniprot/Q3BW34|Q3BW34]] (homology model)
  
 ===== Biological function ===== ===== Biological function =====
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 === How discovered? === === How discovered? ===
  
-XopG was identified based on homology searches using tblastn analysis. Known T3E proteins from plant and animal pathogens were used as query against all contigs of the draft genomes of //X. vesicatoria//, //X. gardneri// and //X. perforans// with e-value of 10<sup>-5 </sup>  (Potnis //et al//., 2011).+XopG was identified based on homology searches using TBLASTN analysis. Known T3E proteins from plant and animal pathogens were used as query against all contigs of the draft genomes of //X. vesicatoria//, //X. gardneri// and //X. perforans// with e-value of 10<sup>-5 </sup>  (Potnis //et al//., 2011).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
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 === Regulation === === Regulation ===
  
-XopG belongs to class B and is still translocated in the absence of HpaB, being constitutively expressed (Thieme, 2006; White// et al//., 2009). XopG was identified as part of the putative HrpX regulon in //X. campestris// pv. //campestris// ATCC339138 (White //et al//., 2009; da Silva //et al//., 2002).+XopG belongs to translocation class B and is still translocated in the absence of HpaB, being constitutively expressed (Schulze et al. 2012). XopG was identified as part of the putative HrpX regulon in //X. campestris// pv. //campestris// ATCC339138 (da Silva //et al//., 2002; White //et al//., 2009).
 === Phenotypes === === Phenotypes ===
  
-A deletion of xopG not display differences in the induction of disease symptoms or hypersensibility response. XopG trigger cell death in different Solanaceae, including //Nicotiana// //tabacum// (White //et al//., 2009). XopG could be an essential pathogenicity factor in pepper (Potnis //et al//., 2011).+A deletion of //xopG// did not display differences in the induction of disease symptoms or hypersensibility response. XopG trigger cell death in different //Solanaceae//, including //Nicotiana// //tabacum// (White //et al//., 2009). XopG could be an essential pathogenicity factor in pepper (Potnis //et al//., 2011). 
 + 
 +//Agrobacterium//-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses, which were not observed when either protein was individually expressed. A screen for //Xanthomonas// effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas// T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).
 === Localization === === Localization ===
  
-Confocal laser scanning microscopy revealed a localization of XopG::GFP exclusively to the plant cell nucleus (White //et al//., 2009).+Confocal laser scanning microscopy revealed a localization of XopG::GFP exclusively to the plant cell nucleus (Schulze et al. 2012). 
 === Enzymatic function === === Enzymatic function ===
  
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 === In xanthomonads === === In xanthomonads ===
  
-Yes (//Xanthomonas// spp.,//X.// //euvesicatoria//, //X. vesicatoria//, //X. gardneri//, //X. campestris// pv. //campestris//, //Xanthomonas campestris// pv. //phaseoli//, //Xanthomonas translucens// pv. //translucens//, //X. translucens// pv. //graminis//, //X. citri pv. bilvae//, //X.citri pv. fuscans//, //X. oryzae// pv. //oryzae//, //X. arboricola// pv. //corylina//, //X. arboricola// pv. //pruni//).+Yes (//e.g.//, //X. arboricola//, //X. campestris//, //X. citri//, //X.// //euvesicatoria//, //X. gardneri//, //X. oryzae////, // //X. translucens//, //X. vesicatoria//).
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
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 da Silva AC, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LM, do Amaral AM, Bertolini MC, Camargo LE, Camarotte G, Cannavan F, Cardozo J, Chambergo F, Ciapina LP, Cicarelli RM, Coutinho LL, Cursino-Santos JR, El-Dorry H, Faria JB, Ferreira AJ, Ferreira RC, Ferro MI, Formighieri EF, Franco MC, Greggio CC, Gruber A, Katsuyama AM, Kishi LT, Leite RP, Lemos EG, Lemos MV, Locali EC, Machado MA, Madeira AM, Martinez-Rossi NM, Martins EC, Meidanis J, Menck CF, Miyaki CY, Moon DH, Moreira LM, Novo MT, Okura VK, Oliveira MC, Oliveira VR, Pereira HA, Rossi A, Sena JA, Silva C, de Souza RF, Spinola LA, Takita MA, Tamura RE, Teixeira EC, Tezza RI, Trindade dos Santos M, Truffi D, Tsai SM, White FF, Setubal JC, Kitajima JP (2002). Comparison of the genomes of two //Xanthomonas// pathogens with differing host specificities. Nature 417: 459-463. DOI: [[https://doi.org/10.1038/417459a|10.1038/417459a]] da Silva AC, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LM, do Amaral AM, Bertolini MC, Camargo LE, Camarotte G, Cannavan F, Cardozo J, Chambergo F, Ciapina LP, Cicarelli RM, Coutinho LL, Cursino-Santos JR, El-Dorry H, Faria JB, Ferreira AJ, Ferreira RC, Ferro MI, Formighieri EF, Franco MC, Greggio CC, Gruber A, Katsuyama AM, Kishi LT, Leite RP, Lemos EG, Lemos MV, Locali EC, Machado MA, Madeira AM, Martinez-Rossi NM, Martins EC, Meidanis J, Menck CF, Miyaki CY, Moon DH, Moreira LM, Novo MT, Okura VK, Oliveira MC, Oliveira VR, Pereira HA, Rossi A, Sena JA, Silva C, de Souza RF, Spinola LA, Takita MA, Tamura RE, Teixeira EC, Tezza RI, Trindade dos Santos M, Truffi D, Tsai SM, White FF, Setubal JC, Kitajima JP (2002). Comparison of the genomes of two //Xanthomonas// pathogens with differing host specificities. Nature 417: 459-463. DOI: [[https://doi.org/10.1038/417459a|10.1038/417459a]]
 +
 +Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the //Xanthomonas// effectors XopQ and XopX results in induction of rice immune responses. Plant J., in press. DOI: [[https://doi.org/10.1111/tpj.14924|10.1111/tpj.14924]]
  
 Potnis N, Krasileva K, Chow V, Almeida NF, Patil PB, Ryan RP, Sharlach M, Behlau F, Dow JM, Momol MT, White FF, Preston JF, Vinatzer BA, Koebnik R, Setubal JC, Norman DJ, Staskawicz BJ, Jones JB (2011). Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper. BMC Genomics 12: 146. DOI: [[https://doi.org/10.1186/1471-2164-12-146|10.1186/1471-2164-12-146]] Potnis N, Krasileva K, Chow V, Almeida NF, Patil PB, Ryan RP, Sharlach M, Behlau F, Dow JM, Momol MT, White FF, Preston JF, Vinatzer BA, Koebnik R, Setubal JC, Norman DJ, Staskawicz BJ, Jones JB (2011). Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper. BMC Genomics 12: 146. DOI: [[https://doi.org/10.1186/1471-2164-12-146|10.1186/1471-2164-12-146]]
bacteria/t3e/xopg.1594226279.txt.gz · Last modified: 2020/07/08 18:37 by rkoebnik

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