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bacteria:t3e:xopn [2020/08/09 20:27]
jvicente 		bacteria:t3e:xopn [2020/08/09 20:32] (current)
jvicente
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 === Regulation === === Regulation ===
  
-Start codon of //xopN// was found downstream of a conserved cis-regulatory element, the plant-inducible promoter (PIP) box (TTCGG-N15-TTCTG). //xopN// is regulated by //hrpX// and //hrpG// genes (Cheong //et al//., 2013; Jiang //et al//., 2008).+Start codon of //xopN// was found downstream of a conserved cis-regulatory element, the plant-inducible promoter (PIP) box (TTCGG-N15-TTCTG). //xopN// is regulated by //hrpX// and //hrpG// genes (Jiang //et al//., 2008; Cheong //et al//., 2013).
  
 qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>  , but this did not apply to //xopN// (Liu //et al.//, 2016). qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>  , but this did not apply to //xopN// (Liu //et al.//, 2016).
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   * XopN has been shown to play a role in host defence systems causing the reduction of PAMP-triggered immune responses and reduce the callose deposition in the host tissue. Moreover the deletion of //xopN//  open reading frame (ORF) reduced the //Xcv//  strain virulence exhibited by lower bacterial spot symptoms occurrence (Kim //et al//., 2009).   * XopN has been shown to play a role in host defence systems causing the reduction of PAMP-triggered immune responses and reduce the callose deposition in the host tissue. Moreover the deletion of //xopN//  open reading frame (ORF) reduced the //Xcv//  strain virulence exhibited by lower bacterial spot symptoms occurrence (Kim //et al//., 2009).
   * The role of XopN in X. oryzae pv. oryzae is dependent on leaf stage (Cheong et al., 2013).   * The role of XopN in X. oryzae pv. oryzae is dependent on leaf stage (Cheong et al., 2013).
-  * XopN has been shown to be required for maximal pathogenicity of //X. axonopodis//  pv. //punicae//  (//Xap//) in pomegranate (Kumar and Mondal, 2013). The deletion of XopN from Xap caused higher accumulation of reactive oxygen species showing that XopN suppresses ROS-mdeiated defense responses during blight pathogenesis in pomegranate (Kumar //et al.//, 2016).+  * XopN has been shown to be required for maximal pathogenicity of //X. axonopodis//  pv. //punicae//  (//Xap//) in pomegranate (Kumar and Mondal, 2013). The deletion of XopN from Xap caused higher accumulation of reactive oxygen species showing that XopN suppresses ROS-mediated defense responses during blight pathogenesis in pomegranate (Kumar //et al.//, 2016).
   * A Δ//xopN//–Δ//xopQ //double knock-out mutant in //X. phaseoli//  pv. //manihotis//  (//Xpm//) was less aggressive in the cassava host plant than its single mutation counterparts. In addition, //in planta //  bacterial growth was reduced at 5 dpi in the double mutant with respect to the wild-type strain CIO151 and individual knock-out strains. The phenotype of the double mutant could be complemented when transforming a plasmid containing //xopQ//. These results confirmed that //xopN //and// xopQ //are functionally redundant in //Xpm//  (Medina //et al.//, 2017).   * A Δ//xopN//–Δ//xopQ //double knock-out mutant in //X. phaseoli//  pv. //manihotis//  (//Xpm//) was less aggressive in the cassava host plant than its single mutation counterparts. In addition, //in planta //  bacterial growth was reduced at 5 dpi in the double mutant with respect to the wild-type strain CIO151 and individual knock-out strains. The phenotype of the double mutant could be complemented when transforming a plasmid containing //xopQ//. These results confirmed that //xopN //and// xopQ //are functionally redundant in //Xpm//  (Medina //et al.//, 2017).
-  * //Agrobacterium// mediated transient transfer of the gene for XopN resulted in suppression of rice innate immune responses induced by LipA, a hydrolitic enzyme secreted by //X. oryzae//  pv. //oryzae//  (Xoo), but a //xopN// <sup>//-// </sup>  mutant of //Xoo// retains the ability to suppress these innate immune responses indicating other functionally redundant proteins; amongst these proteins, XopQ, XopX and XopZ are suppressors of LipA induced innate immune responses; mutation in any one of the //xopN, xopQ, xopX or xopZ// genes causes partial virulence deficiency (Sinha et al., 2013). XopN was shown to contribute significantly to //X. oryzae//  pv. //oryzae//  (Xoo) virulence on a susceptible rice variety Nipponbare. XopN was shown to be highly translocated to suppress rice defense responses (Mo //et al.//, 2020).+  * //Agrobacterium//  mediated transient transfer of the gene for XopN resulted in suppression of rice innate immune responses induced by LipA, a hydrolitic enzyme secreted by //X. oryzae//  pv. //oryzae//  (Xoo), but a //xopN// <sup>//-// </sup>   mutant of //Xoo//  retains the ability to suppress these innate immune responses indicating other functionally redundant proteins; XopQ, XopX and XopZ were shown to be suppressors of LipA induced innate immune responses; mutation in any one of the //xopN, xopQ, xopX or xopZ//  genes causes partial virulence deficiency (Sinha et al., 2013). XopN was shown to contribute significantly to //X. oryzae//  pv. //oryzae//  (Xoo) virulence on a susceptible rice variety Nipponbare. XopN was shown to be highly translocated to suppress rice defense responses (Mo //et al.//, 2020).
   * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae//  pv. //oryzicola//  (Xoc GX01) (Liao //et al.//, 2020).   * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae//  pv. //oryzicola//  (Xoc GX01) (Liao //et al.//, 2020).
  
bacteria/t3e/xopn.1596997668.txt.gz · Last modified: 2020/08/09 20:27 by jvicente

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