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bacteria:t3e:xopp [2022/06/21 16:49]
rkoebnik [Biological function] 		bacteria:t3e:xopp [2022/06/23 09:55] (current)
rkoebnik [Biological function]
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 === How discovered? === === How discovered? ===
  
-XopP was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//)strain 85-10. The XopP::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopP was also identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_2994 (Jiang //et al.//, 2009).+XopP was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//) strain 85-10. The XopP::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopP was also identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_2994 (Jiang //et al.//, 2009).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
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   * XopP<sub>Xoo</sub>  is able to suppress rice pathogen associated molecular pattern (PAMP)-immunity and resistance to //Xanthomonas oryzae//  pv. //oryzae//. Although XopP<sub>Xoo</sub>  is classified within the XopP, it shows only 40% sequence identity with the XopP homologue of //X. campestris//  pv. //campestris//  (Furutani //et al//., 2009). Therefore, it remains unclear if such interaction is similar in different pathosystems where XopP has been found.   * XopP<sub>Xoo</sub>  is able to suppress rice pathogen associated molecular pattern (PAMP)-immunity and resistance to //Xanthomonas oryzae//  pv. //oryzae//. Although XopP<sub>Xoo</sub>  is classified within the XopP, it shows only 40% sequence identity with the XopP homologue of //X. campestris//  pv. //campestris//  (Furutani //et al//., 2009). Therefore, it remains unclear if such interaction is similar in different pathosystems where XopP has been found.
   * //Agrobacterium//-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses, which were not observed when either protein was individually expressed. A screen for //Xanthomonas//  effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas//  T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).   * //Agrobacterium//-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses, which were not observed when either protein was individually expressed. A screen for //Xanthomonas//  effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas//  T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).
-  * XopP inhibits the function of the host-plant exocyst complex by direct targeting of Exo70B, a subunit of the exocyst complex, which plays a significant role in plant immunity. XopP interferes with exocyst-dependent exocytosis, and can do this without activating a plant NLR (NOD-like receptor) that guards Exo70B in Arabidopsis. In this way, //Xanthomonas//  efficiently inhibits the host's pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) by blocking exocytosis of pathogenesis-related protein-1A (PR1a), callose deposition and localization of the FLS2 immune receptor to the plasma membrane, thus promoting successful infection.+  * XopP inhibits the function of the host-plant exocyst complex by direct targeting of Exo70B, a subunit of the exocyst complex, which plays a significant role in plant immunity. XopP interferes with exocyst-dependent exocytosis, and can do this without activating a plant NLR (NOD-like receptor) that guards Exo70B in Arabidopsis. In this way, //Xanthomonas//  efficiently inhibits the host's pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) by blocking exocytosis of pathogenesis-related protein-1A (PR1a), callose deposition and localization of the FLS2 immune receptor to the plasma membrane, thus promoting successful infection (Michalopoulou //et al.//, 2022).
  
 === Localization === === Localization ===
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 === Interaction partners === === Interaction partners ===
  
-XopP<sub>Xoo</sub>  interacts with the U-box domain of a rice ubiquitin E3 ligase, OsPUB44 and inhibits its activity (Ishikawa //et al//., 2014).+XopP<sub>Xoo</sub>  interacts with the U-box domain of a rice ubiquitin E3 ligase, OsPUB44 and inhibits its activity (Ishikawa //et al//., 2014). XopP<sub>Xcc</sub>  interacts with EXO70B1, EXO70B2 and EXO70F1 in a yeast two-hybrid assay (Michalopoulou //et al.//, 2022). Interaction was confirmed in planta by split YFP and coIP assays (Michalopoulou //et al.//, 2022).
  
 ===== Conservation ===== ===== Conservation =====
bacteria/t3e/xopp.1655822959.txt.gz ยท Last modified: 2022/06/21 16:49 by rkoebnik

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