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--- bacteria:t3e:xopp [2022/06/23 09:53]
rkoebnik [Biological function]
+++ bacteria:t3e:xopp [2022/06/23 09:55] (current)
rkoebnik [Biological function]
@@ Line 15: / Line 15: @@
 === How discovered? ===
-XopP was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//)strain 85-10. The XopP::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopP was also identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_2994 (Jiang //et al.//, 2009).
+XopP was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//) strain 85-10. The XopP::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopP was also identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_2994 (Jiang //et al.//, 2009).
 === (Experimental) evidence for being a T3E ===
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 === Interaction partners ===
-XopP<sub>Xoo</sub>  interacts with the U-box domain of a rice ubiquitin E3 ligase, OsPUB44 and inhibits its activity (Ishikawa //et al//., 2014). XopP interacts with EXO70B1 in a yeast two-hybrid assay and in planta, as assessed by split YFP and coIP assays (Michalopoulou //et al.//, 2022).
+XopP<sub>Xoo</sub>  interacts with the U-box domain of a rice ubiquitin E3 ligase, OsPUB44 and inhibits its activity (Ishikawa //et al//., 2014). XopP<sub>Xcc</sub>  interacts with EXO70B1, EXO70B2 and EXO70F1 in a yeast two-hybrid assay (Michalopoulou //et al.//, 2022). Interaction was confirmed in planta by split YFP and coIP assays (Michalopoulou //et al.//, 2022).
 ===== Conservation =====

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