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bacteria:t3e:xopx [2020/07/21 11:39] rkoebnik [XopX] |
bacteria:t3e:xopx [2020/10/27 19:36] rkoebnik |
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====== XopX ====== | ====== XopX ====== | ||
- | Author: [[https:// | + | Author: [[https:// |
Internal reviewer: Coline Sciallano\\ | Internal reviewer: Coline Sciallano\\ | ||
- | Expert reviewer: | + | Expert reviewer: |
Class: XopX\\ | Class: XopX\\ | ||
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=== How discovered? === | === How discovered? === | ||
- | XopX was discovered through the screening of a genomic cosmid library of //X. euvesicatoria// | + | XopX was discovered through the screening of a genomic cosmid library of //X. euvesicatoria// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
Line 25: | Line 25: | ||
* XopX was demonstrated to be cytotoxic when expressed in yeast, suggesting it may target a conserved eukaryotic cell process required for cell viability (Salomon //et al//., 2011). | * XopX was demonstrated to be cytotoxic when expressed in yeast, suggesting it may target a conserved eukaryotic cell process required for cell viability (Salomon //et al//., 2011). | ||
- | * During infection of rice (//Oryzae | + | * During infection of rice <font 10.5pt/ |
- | * XopX is required for the development of //X. euvesicatoria// | + | * XopX is required for the development of //X. euvesicatoria// |
- | * A ∆//xopK// mutant strain of // | + | * <font 10.5pt/ |
- | * // | + | * When expressed in // |
- | * A screen for // | + | * When transiently expressed in //N. benthamiana by Agrobacterium tumefaciens// |
+ | * //A ∆xopX// mutant strain of // | ||
+ | * // | ||
+ | * A screen for // | ||
+ | * <font 10.5pt/ | ||
+ | * <font 10.5pt/ | ||
+ | * <font 10.5pt/ | ||
=== Localization === | === Localization === | ||
- | Unknown. | + | // |
=== Enzymatic function === | === Enzymatic function === | ||
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=== Interaction partners === | === Interaction partners === | ||
- | It has been suggested that XopX-triggering of plant cell death response was dependent | + | * It has been suggested that XopX-triggering of plant cell death response was dependent |
+ | * <font 10.5pt/ | ||
+ | * <font 10.5pt/ | ||
- | XopX interacts with two of the eight rice 14-3-3 proteins. Mutants of XopX that are defective in 14-3-3 binding are also defective in suppression of immune responses, suggesting that interaction with 14-3-3 proteins is required for suppression of host innate immunity (Deb //et al.//, 2020). | + | === Conservation === |
- | Yeast two-hybrid, bimolecular fluorescence complementation (BiFC) and co-IP assays indicated that XopQ and XopX interact with each other (Deb //et al.//, 2020). | + | **In xanthomonads** |
- | ===== Conservation ===== | + | Yes, xopX homologs can be found in almost every sequenced // |
- | === In xanthomonads === | + | **In other plant pathogens/ |
- | Yes, // | + | Related proteins (query cover > 80% and percent identity > 50 %) can be detected |
- | === In other plant pathogens/ | + | === References |
- | Related proteins | + | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the Xanthomonas effectors XopQ and XopX results |
- | ===== References ===== | + | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // |
- | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // | + | Liu Y, Long J, Shen D, Song C (2016). // |
- | Jiang BL, He YQ, Cen WJ, Wei HY, Jiang GF, Jiang W, Hang XH, Feng JX, Lu GT, Tang DJ, Tang JL (2008). The type III secretion effector XopXccN of // | + | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // |
- | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in Xanthomonas | + | Metz M, Dahlbeck D, Morales CQ, Sady BA, Clark ET, Staskawicz BJ (2005). The conserved// |
- | Lindeberg M, Cunnac S, Collmer A (2012). // | + | Mutka AM, Fentress SJ, Sher JW, Berry JC, Pretz C, Nusinow DA, Bart R (2016). Quantitative, |
- | Liu Y, Long J, Shen D, Song C (2016). // | + | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of // |
- | + | ||
- | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors | + | |
- | + | ||
- | Metz M, Dahlbeck D, Morales CQ, Sady BA, Clark ET, Staskawicz BJ (2005). The conserved // | + | |
- | + | ||
- | Mutka AM, Fentress SJ, Sher JW, Berry JC, Pretz C, Nusinow DA, Bart R (2016). Quantitative, | + | |
- | Salomon | + | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // |
- | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // | + | <font 10.5pt/ |
- | Stork W, Kim JG, Mudgett MB (2015). Functional analysis of plant defense suppression and activation by the //Xanthomonas// | + | Stork W, Kim JG, Mudgett MB (2015). Functional analysis of plant defense suppression and activation by the Xanthomonas core type III effector XopX. Mol. Plant. Microbe Interact. 28: 180-194. DOI: [[https:// |