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bacteria:t3e:xopx [2020/10/22 20:32] sonti |
bacteria:t3e:xopx [2021/01/05 17:26] (current) rkoebnik [Biological function] |
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====== XopX ====== | ====== XopX ====== | ||
- | Author: [[https:// | + | Author: [[https:// |
Internal reviewer: Coline Sciallano\\ | Internal reviewer: Coline Sciallano\\ | ||
Expert reviewer: Ramesh V. Sonti | Expert reviewer: Ramesh V. Sonti | ||
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=== How discovered? === | === How discovered? === | ||
- | XopX was discovered through the screening of a genomic cosmid library of //X. euvesicatoria// | + | XopX was discovered through the screening of a genomic cosmid library of //X. euvesicatoria// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
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* XopX was demonstrated to be cytotoxic when expressed in yeast, suggesting it may target a conserved eukaryotic cell process required for cell viability (Salomon //et al//., 2011). | * XopX was demonstrated to be cytotoxic when expressed in yeast, suggesting it may target a conserved eukaryotic cell process required for cell viability (Salomon //et al//., 2011). | ||
- | * During infection of rice <font 10.5pt/ | + | * During infection of rice <font 10.5pt/ |
- | * XopX is required for the development of //X. euvesicatoria// | + | * XopX is required for the development of //X. euvesicatoria// |
- | * <font 10.5pt/ | + | * <font 10.5pt/ |
- | * When expressed in Pseudomonas fluorescens //55 (Pf //55), a non‐pathogenic bacterium capable of eliciting PTI (callose deposit) in Arabidopsis //Col‐0 plants, XopX is not able to reduce callose deposit, suggesting in these conditions, the effector is not able to suppress PTI. Using the same heterologuous system, XopX is not able to suppress the HR triggered by Pf //55 HopA1 on tobacco, suggesting that is these conditions, XopX do not act as an ETI suppressor. (Medina et al//., 2018). | + | * When expressed in //Pseudomonas fluorescens //55 (//Pf //55), a non‐pathogenic bacterium capable of eliciting PTI (callose deposit) in //Arabidopsis //Col‐0 plants, XopX is not able to reduce callose deposit, suggesting in these conditions, the effector is not able to suppress PTI. Using the same heterologuous system, XopX is not able to suppress the HR triggered by Pf 55 HopA1 on tobacco, suggesting that is these conditions, XopX do not act as an ETI suppressor. (Medina |
- | * When transiently expressed in N. benthamina// by Agrobacterium tumefaciens// | + | * When transiently expressed in //N. benthamiana |
- | * A ∆xopX// mutant strain of Xanthomonas phaseoli// | + | * //A ∆xopX// |
- | * Agrobacterium// | + | * //Agrobacterium// |
- | * A screen for Xanthomonas//<font 10.5pt/ | + | * A screen for //Xanthomonas |
- | * <font 10.5pt/ | + | * <font 10.5pt/ |
- | * <font 10.5pt/ | + | * <font 10.5pt/ |
- | * <font 10.5pt/ | + | * <font 10.5pt/ |
=== Localization === | === Localization === | ||
- | | + | //Xanthomonas oryzae |
=== Enzymatic function === | === Enzymatic function === | ||
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=== Interaction partners === | === Interaction partners === | ||
- | It has been suggested that XopX-triggering of plant cell death response was dependent | + | * It has been suggested that XopX-triggering of plant cell death response was dependent |
- | + | | |
- | <font 10.5pt/ | + | |
- | + | ||
- | <font 10.5pt/ | + | |
- | + | ||
- | ===== Conservation ===== | + | |
- | === In xanthomonads | + | === Conservation |
- | Yes, // | + | **In xanthomonads** |
- | === In other plant pathogens/symbionts === | + | Yes, xopX homologs can be found in almost every sequenced |
- | Related proteins (query cover > 80% and percent identity > 50 %) can be detected in several unclassified | + | **In other plant pathogens/symbionts ** |
- | ===== References ===== | + | Related proteins (query cover > 80% and percent identity > 50 %) can be detected in several unclassified Burkholderiales (// |
- | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // | + | === References === |
- | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 | + | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the Xanthomonas effectors XopQ and XopX results |
- | Lindeberg M, Cunnac | + | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in //Xanthomonas oryzae |
- | Liu Y, Long J, Shen D, Song C (2016). // | + | Liu Y, Long J, Shen D, Song C (2016). // |
- | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | + | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // |
- | Metz M, Dahlbeck D, Morales CQ, Sady BA, Clark ET, Staskawicz BJ (2005). The conserved // | + | Metz M, Dahlbeck D, Morales CQ, Sady BA, Clark ET, Staskawicz BJ (2005). The conserved// Xanthomonas campestris// |
Mutka AM, Fentress SJ, Sher JW, Berry JC, Pretz C, Nusinow DA, Bart R (2016). Quantitative, | Mutka AM, Fentress SJ, Sher JW, Berry JC, Pretz C, Nusinow DA, Bart R (2016). Quantitative, | ||
- | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of // | + | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of // |
- | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // | + | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // |
- | <font 10.5pt/ | + | <font 10.5pt/ |
- | Stork W, Kim JG, Mudgett MB (2015). Functional analysis of plant defense suppression and activation by the //Xanthomonas// | + | Stork W, Kim JG, Mudgett MB (2015). Functional analysis of plant defense suppression and activation by the Xanthomonas core type III effector XopX. Mol. Plant. Microbe Interact. 28: 180-194. DOI: [[https:// |