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--- bacteria:t3e:xopx [2020/10/22 21:07]
sonti
+++ bacteria:t3e:xopx [2021/01/05 17:26] (current)
rkoebnik [Biological function]
@@ Line 1: / Line 1: @@
 ====== XopX ======
-Author: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]] & Sohini Deb\\
+Author: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]] & [[https://www.researchgate.net/profile/Sohini_Deb|Sohini Deb]]\\
 Internal reviewer: Coline Sciallano\\
 Expert reviewer: Ramesh V. Sonti
@@ Line 15: / Line 15: @@
 === How discovered? ===
-XopX was discovered through the screening of a genomic cosmid library of //X. euvesicatoria// strain GM98-38 conjugated in //X. campestris// pv. //campestris// that allowed //Xcc// to elicit an //Xcv // cell death-like response when inoculated on //N. benthamiana// . (Metz // et al//., 2005).
+XopX was discovered through the screening of a genomic cosmid library of //X. euvesicatoria// strain GM98-38 conjugated in //X. campestris// pv. //campestris// that allowed //Xcc// to elicit an //Xcv // cell death-like response when inoculated on //N. benthamiana// (Metz // et al//., 2005).
 === (Experimental) evidence for being a T3E ===
@@ Line 26: / Line 26: @@
   * XopX was demonstrated to be cytotoxic when expressed in yeast, suggesting it may target a conserved eukaryotic cell process required for cell viability (Salomon //et al//., 2011).
   * During infection of rice <font 10.5pt/inherit;;#333333;;inherit>(//Oryza sativa//</font>) with //Xanthomonas oryzae//  pv. //oryzae//, XopX was shown to be an inhibitor of rice innate immune response, as it suppresses LipA-induced callose deposition (Sinha //et al//., 2013).
-  * XopX is required for the development of //X. euvesicatoria//-induced symptoms in the bacterial spot disease of tomato (//Solanum lycopersicum//) and pepper (//Capsicum annuum//). Indeed, it promotes ethylene production, and therefore chlorosis and plant cell death during infection by //X. euvesicatoria//  of susceptible tomato and in transient expression assays in tobacco. Interestingly, it also suppresses flagellin-induced production of reactive oxygen species (ROS) while promoting the accumulation of pattern-triggered immunity (PTI) gene transcripts (Stork //et al//., 2015). Eventually, the complex behavior of XopX //in planta//, which combines activation and suppression of immunity-related plant responses at the same time, allows to classify this effector with the T3Es that activates the plant ‘default to death and defense’ response (Lindeberg //et al//., 2012; Stork //et al//., 2015).
+  * XopX is required for the development of //X. euvesicatoria//- induced symptoms in the bacterial spot disease of tomato (//Solanum lycopersicum//) and pepper (//Capsicum annuum//). Indeed, it promotes ethylene production, and therefore chlorosis and plant cell death during infection by //X. euvesicatoria//  of susceptible tomato and in transient expression assays in tobacco. Interestingly, it also suppresses flagellin-induced production of reactive oxygen species (ROS) while promoting the accumulation of pattern-triggered immunity (PTI) gene transcripts (Stork //et al//., 2015). Eventually, the complex behavior of XopX //in planta//, which combines activation and suppression of immunity-related plant responses at the same time, allows to classify this effector with the T3Es that activates the plant ‘default to death and defense’ response (Lindeberg //et al//., 2012; Stork //et al//., 2015).
-  * <font 10.5pt/inherit;;#333333;;inherit>XopX is required for full virulence in //Xanthomonas //</font>//<font 10.5pt/inherit;;#333333;;inherit>axonopodis</font>// //<font 10.5pt/inherit;;#333333;;inherit>pv. manihotis</font><font 10.5pt/inherit;;#333333;;inherit>CIO151</font>//<font 10.5pt/inherit;;#333333;;inherit>strain</font>//<font 10.5pt/inherit;;#333333;;inherit>. A</font> Xam//  strain deleted for //xopX//  showed decreased ability to produce symptoms in leaves of cassava. Also, the// xopX //KO reached more than one log unit lower populations than those observed for the wild‐type strain. This underlies the importance of this effector for disease developement (Medina //et al., //2018).
+  * <font 10.5pt/inherit;;#333333;;inherit>XopX is required for full virulence in //Xanthomonas //</font>//<font 10.5pt/inherit;;#333333;;inherit>axonopodis</font>// //<font 10.5pt/inherit;;#333333;;inherit>pv. manihotis</font>//<font 10.5pt/inherit;;#333333;;inherit>CIO151 strain</font>//<font 10.5pt/inherit;;#333333;;inherit>. A</font> Xam//  strain deleted for //xopX//  showed decreased ability to produce symptoms in leaves of cassava. Also, the// xopX //KO reached more than one log unit lower populations than those observed for the wild‐type strain. This underlies the importance of this effector for disease developement (Medina //et al., //2018).
   * When expressed in //Pseudomonas fluorescens //55 (//Pf //55), a non‐pathogenic bacterium capable of eliciting PTI (callose deposit) in //Arabidopsis //Col‐0 plants, XopX is not able to reduce callose deposit, suggesting in these conditions, the effector is not able to suppress PTI. Using the same heterologuous system, XopX is not able to suppress the HR triggered by Pf 55 HopA1 on tobacco, suggesting that is these conditions, XopX do not act as an ETI suppressor. (Medina //et al//., 2018).
-  * When transiently expressed in //N. benthamiana by Agrobacterium tumefaciens//–mediated expression system, XopX from X. oryzae// pv. oryzicola//  cause the nonhost HR at approximately 2 days (Li// et al., //2015).
+  * When transiently expressed in //N. benthamiana by Agrobacterium tumefaciens//–mediated expression system, XopX from //X. oryzae //pv. //oryzicola//  cause the nonhost HR at approximately 2 days (Li// et al., //2015).
-  * //A ∆xopX//  mutant strain of //Xanthomonas phaseoli //pv. //manihotis//  (aka //Xanthomonas axonopodis //pv. //manihotis//) showed reduced growth in planta and delayed spread through the vasculature system of cassava (Mutka et al.//, 2016). //
+  * //A ∆xopX//  mutant strain of //Xanthomonas phaseoli //pv. //manihotis//  (aka //Xanthomonas axonopodis //pv. //manihotis//) showed reduced growth //in planta//  and delayed spread through the vasculature system of cassava (Mutka et al.//, 2016). //
-  * //Agrobacterium//- mediated transient expression of both //<font 10.5pt/inherit;;#333333;;inherit>Xanthomonas</font><font 10.5pt/inherit;;#333333;;inherit>//or//yzae</font>//<font 10.5pt/inherit;;#333333;;inherit>pv.</font>//<font 10.5pt/inherit;;#333333;;inherit>oryzae</font> //XopQ and XopX in rice cells resulted in induction of rice immune responses. These immune responses were not observed when either protein was individually expressed in rice cells. XopQ-XopX induced rice immune responses were not observed with a XopX mutant that is defective in 14-3-3 binding (Deb //et al.//, 2020).
+  * //Agrobacterium//- mediated transient expression of both //<font 10.5pt/inherit;;#333333;;inherit>Xanthomonas</font>oryzae //<font 10.5pt/inherit;;#333333;;inherit>pv.</font>//<font 10.5pt/inherit;;#333333;;inherit>oryzae</font>//XopQ and XopX in rice cells resulted in induction of rice immune responses. These immune responses were not observed when either protein was individually expressed in rice cells. XopQ-XopX induced rice immune responses were not observed with a XopX mutant that is defective in 14-3-3 binding (Deb //et al.//, 2020).
   * A screen for //Xanthomonas <font 10.5pt/inherit;;#333333;;inherit>oryzae</font>//<font 10.5pt/inherit;;#333333;;inherit>pv.// oryzae//</font> effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas //T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).
   * <font 10.5pt/inherit;;#333333;;inherit>XopX</font><font 10.5pt/inherit;;#333333;;inherit>(ACD57163) is one among the five classes of virulence genes found to be upregulated in the</font>//<font 10.5pt/inherit;;#333333;;inherit>Xanthomonas oryzae</font>//<font 10.5pt/inherit;;#333333;;inherit>pv.</font>//<font 10.5pt/inherit;;#333333;;inherit>oryzae</font>//<font 10.5pt/inherit;;#333333;;inherit>MAI1 strain during infection.</font>//<font inherit/inherit;;black;;white>xopX</font>//<font inherit/inherit;;black;;white>was up-regulated at both 3 and 6 dai</font><font 10.5pt/inherit;;#333333;;inherit>(Soto-Suárez //et al.//</font>, 2010)<font inherit/inherit;;black;;white>.</font>
   * <font 10.5pt/inherit;;#333333;;inherit>The //xopX// gene contributes to the virulence of //Xanthomonas campestris// pv. //vesicatoria //on hosts pepper and tomato. XopX targets the innate immune response, resulting in enhanced plant disease susceptibility (Metz //et al//</font>//.//, 2005).
-  * <font 10.5pt/inherit;;#333333;;inherit>A //Xanthomonas campestris//</font><font 10.5pt/inherit;;#333333;;inherit>pv.</font>//<font 10.5pt/inherit;;#333333;;inherit>vesicatoria</font>//<font 10.5pt/inherit;;#333333;;inherit>xopX</font><font 10.5pt/inherit;;#333333;;inherit>mutant strain could not induce cell death response in</font>//<font 10.5pt/inherit;;#333333;;inherit>N. benthamiana</font>//<font 10.5pt/inherit;;#333333;;inherit>, and could be complemented back to cell death response on</font>//<font 10.5pt/inherit;;#333333;;inherit>N. benthamiana</font>//<font 10.5pt/inherit;;#333333;;inherit>in trans with plasmid subclones of</font>//<font 10.5pt/inherit;;#333333;;inherit>xopX</font>//<font 10.5pt/inherit;;#333333;;inherit>(Metz// et al//</font>////.//, //2005).
+  * <font 10.5pt/inherit;;#333333;;inherit>A //Xanthomonas campestris //</font><font 10.5pt/inherit;;#333333;;inherit>pv.</font>//<font 10.5pt/inherit;;#333333;;inherit>vesicatoria</font>//<font 10.5pt/inherit;;#333333;;inherit>//xopX //</font><font 10.5pt/inherit;;#333333;;inherit>mutant strain could not induce cell death response in</font>//<font 10.5pt/inherit;;#333333;;inherit>N. benthamiana</font>//<font 10.5pt/inherit;;#333333;;inherit>, and could be complemented back to cell death response on</font>//<font 10.5pt/inherit;;#333333;;inherit>N. benthamiana</font>//<font 10.5pt/inherit;;#333333;;inherit>in trans with plasmid subclones of</font>//<font 10.5pt/inherit;;#333333;;inherit>xopX</font>//<font 10.5pt/inherit;;#333333;;inherit>(Metz// et al//</font>.//, //2005).
-===   Localization   ===
+=== Localization ===
-//Xanthomonas oryzae //pv. //oryzae //XopX wild-type protein localizes mostly to the nucleus, but is also present to a lesser extent in the peripheral cytoplasm. However, the XopX S193A and XopX S477A mutants of XopX, that are defective in binding to the rice 14-3-3 proteins GF14d and GF14e, were found to be unable to localize to the nucleus, and were mostly observed in the cytoplasm (Deb et al. , 2020).
+//Xanthomonas oryzae //pv. //oryzae //XopX wild-type protein localizes mostly to the nucleus, but is also present to a lesser extent in the peripheral cytoplasm. However, the XopX S193A and XopX S477A mutants of XopX, that are defective in binding to the rice 14-3-3 proteins GF14d and GF14e, were found to be unable to localize to the nucleus, and were mostly observed in the cytoplasm (Deb //et al.//, 2020).
-===   Enzymatic function   ===
+=== Enzymatic function ===
 Unknown.
-===   Interaction partners   ===
+=== Interaction partners ===
   * It has been suggested that XopX-triggering of plant cell death response was dependent on another cofactor delivered by the T3SS, yet still unknown (Metz //et al.//, 2005).
@@ Line 63: / Line 63: @@
 === References ===
-Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responses. <font 10.5pt/inherit;;#333333;;inherit>Plant J., 104: 332-350.</font> DOI: [[https://doi.org/10.1111/tpj.14924|10.1111/tpj.14924]]
+Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responses. Plant J. 104: 332-350<font 10.5pt/inherit;;#333333;;inherit>.</font> DOI: [[https://doi.org/10.1111/tpj.14924|10.1111/tpj.14924]]
 Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in //Xanthomonas oryzae //pv. //oryzicola //suppresses rice immunity and promotes disease development. Mol. Plant Microbe Interact. 28: 869-880. DOI: [[https://doi.org/10.1094/MPMI-10-14-0314-R|10.1094/MPMI-10-14-0314-R]] Lindeberg M, Cunnac S, Collmer A (2012). Pseudomonassyringae type III effector repertoires: last words in endless arguments. Trends Microbiol. 20: 199-208. DOI: [[https://doi.org/10.1016/j.tim.2012.01.003|10.1016/j.tim.2012.01.003]]

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