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bacteria:t3e:xopy [2020/06/10 12:18] rkoebnik [References] |
bacteria:t3e:xopy [2020/07/09 11:57] rkoebnik [XopY] |
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====== XopY ====== | ====== XopY ====== | ||
- | Author: Irena Mačionienė\\ | + | Author: |
- | Internal reviewer: Lucas Morinière\\ | + | Internal reviewer: |
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
Class: XopY\\ | Class: XopY\\ | ||
Family: XopY\\ | Family: XopY\\ | ||
- | Prototype: | + | Prototype: |
- | RefSeq ID: [[https:// | + | RefSeq ID: [[https:// |
+ | 3D structure: Unknown | ||
===== Biological function ===== | ===== Biological function ===== | ||
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=== How discovered? === | === How discovered? === | ||
- | XopY was discovered by screening the genome of //X. oryzae //pv. //oryzae// MAFF311018 for proteins displaying a N-terminal amino acid pattern associated with T3S substrates in // | + | XopY was discovered by screening the genome of //X. oryzae //pv. //oryzae// MAFF311018 for proteins displaying a N-terminal amino acid pattern associated with T3S substrates in // |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | //X. oryzae //pv. //oryzae // | + | //X. oryzae //pv. //oryzae // |
=== Regulation === | === Regulation === | ||
- | XopY from //X. oryzae //pv. //oryzae// posseses a PIP and ‐10 box in the promoter region (TTCGB‐N< | + | XopY from //X. oryzae //pv. //oryzae// posseses a PIP and ‐10 box in the promoter region (TTCGB‐N< |
+ | |||
+ | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the // | ||
=== Phenotypes === | === Phenotypes === | ||
- | Transgenic rice plants expressing XOO1488 (Xoo1488-OX) were generated and inoculated with the T3SS-deficient //hrpX// mutant of //Xoo//, which is incapable of type III effector delivery. The //Xoo hrpX// mutant did not cause lesions in wild-type plant, presumably because of strong induction of PTI. In contrast, Xoo1488-OX plants had severe disease symptoms following infection with the //Xoo hrpX// mutant. Bacterial populations of the //Xoo hrpX// mutant in Xoo1488-OX leaves were higher than in wild-type plants. The growth of wild-type isolate Xoo MAFF311018 in Xoo1488-OX plants also increased significantly over its growth in wild-type plants. Thus, it is likely that XopY inhibits PTI induced by infection of //Xoo hrpX// mutant. However, it was noted that XopY knockout strain did not exhibit any defect in virulence (Yamaguchi //et al//., 2013a). XopY was also shown to inhibit chitin-induced expression of defense-related genes (Yamaguchi //et al. //2013b). XopY of //X. oryzae //pv. // | + | Transgenic rice plants expressing XOO1488 (Xoo1488-OX) were generated and inoculated with the T3SS-deficient //hrpX// mutant of //Xoo//, which is incapable of type III effector delivery. The //Xoo hrpX// mutant did not cause lesions in wild-type plant, presumably because of strong induction of PTI. In contrast, Xoo1488-OX plants had severe disease symptoms following infection with the //Xoo hrpX// mutant. Bacterial populations of the //Xoo hrpX// mutant in Xoo1488-OX leaves were higher than in wild-type plants. The growth of wild-type isolate |
=== Localization === | === Localization === | ||
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XopY was demonstrated to target OsRLCK185. Expression of XopY in rice cells compromises OsRLCK185-mediated immune responses, which is consistent with the fact that Xoo1488 inhibits trans-phosphorylation of the activation domain of OsRLCK185 by OsCERK1. Interestingly, | XopY was demonstrated to target OsRLCK185. Expression of XopY in rice cells compromises OsRLCK185-mediated immune responses, which is consistent with the fact that Xoo1488 inhibits trans-phosphorylation of the activation domain of OsRLCK185 by OsCERK1. Interestingly, | ||
- | |||
===== Conservation ===== | ===== Conservation ===== | ||
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===== References ===== | ===== References ===== | ||
- | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in// Xanthomonas oryzae// pv. //oryzae//. Mol. Plant Microbe Interact. 22: 96-106. DOI:</ | + | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in// Xanthomonas oryzae// pv. //oryzae//. Mol. Plant Microbe Interact. 22: 96-106. DOI: [[https:// |
Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // | ||
+ | |||
+ | Liu Y, Long J, Shen D, Song C (2016). // | ||
Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< | ||
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Yamaguchi K, Nakamura Y, Ishikawa K, Yoshimura Y, Tsuge S, Kawasaki T (2013a). Suppression of rice immunity by // | Yamaguchi K, Nakamura Y, Ishikawa K, Yoshimura Y, Tsuge S, Kawasaki T (2013a). Suppression of rice immunity by // | ||
- | Yamaguchi K, Yamada K, Ishikawa K, Yoshimura S, Hayashi N, Uchihashi K, Ishihama N, Kishi-Kaboshi M, Takahashi A, Tsuge S, Ochiai H, Tada Y, Shimamoto K, Yoshioka H, Kawasaki T (2013b). A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity. Cell Host Microbe 13: 347-357. DOI: [[https:// | + | Yamaguchi K, Yamada K, Ishikawa K, Yoshimura S, Hayashi N, Uchihashi K, Ishihama N, Kishi-Kaboshi M, Takahashi A, Tsuge S, Ochiai H, Tada Y, Shimamoto K, Yoshioka H, Kawasaki T (2013b). A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity. Cell Host Microbe 13: 347-357. DOI: [[https:// |