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--- bacteria:t3e:xopy [2020/07/02 16:03]
rkoebnik [Biological function]
+++ bacteria:t3e:xopy [2020/07/09 12:13] (current)
rkoebnik
@@ Line 1: / Line 1: @@
 ====== XopY ======
-Author: Irena Mačionienė\\
+Author: [[https://www.researchgate.net/profile/Irena_Macioniene|Irena Mačionienė]]\\
-Internal reviewer: Lucas Morinière\\
+Internal reviewer: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]]\\
 Expert reviewer: FIXME
 Class: XopY\\
 Family: XopY\\
-Prototype: XopY, aka Xoo1488 (//Xanthomonas oryzae// pv. //oryzicola// BLS256)\\
+Prototype: XOO1488 (//Xanthomonas oryzae// pv. //oryzae//; strain MAFF 311018)\\
-RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/AEQ97580.1|AEQ97580]] (276 aa)
+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/AEQ97580.1|AEQ97580]] (276 aa)\\
 D structure: Unknown
@@ Line 15: / Line 15: @@
 === How discovered? ===
-XopY was discovered by screening the genome of //X. oryzae //pv. //oryzae// MAFF311018 for proteins displaying a N-terminal amino acid pattern associated with T3S substrates in //Pseudomonas syringae// (Furutani //et al.//, 2009). It has been primarily referred to as XOO1488, and then XopY (Song & Yang, 2010).
+XopY was discovered by screening the genome of //X. oryzae //pv. //oryzae// MAFF 311018 for proteins displaying a N-terminal amino acid pattern associated with T3S substrates in //Pseudomonas syringae// (Furutani //et al.//, 2009). It has been primarily referred to as XOO1488, and then XopY (Song & Yang, 2010).
 === (Experimental) evidence for being a T3E ===
@@ Line 23: / Line 23: @@
 XopY from //X. oryzae //pv. //oryzae// posseses a PIP and ‐10 box in the promoter region (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Yamaguchi //et al//., 2013a). Also, it was shown to be regulated by HrpX (Furutani //et al.//, 2009).
-qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//XrvC// mutant compared with those in the wild type strain PXO99<sup>A</sup>  (Liu //et al.//, 2016).
+qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>  (Liu //et al.//, 2016).
 === Phenotypes ===
@@ Line 38: / Line 38: @@
 XopY was demonstrated to target OsRLCK185. Expression of XopY in rice cells compromises OsRLCK185-mediated immune responses, which is consistent with the fact that Xoo1488 inhibits trans-phosphorylation of the activation domain of OsRLCK185 by OsCERK1. Interestingly, XopY is phosphorylated by OsRLCK185, suggesting that modification of XopY in host cell may affect their virulent activity (Yamaguchi //et al//., 2013b).
 ===== Conservation =====
@@ Line 53: / Line 52: @@
 Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in //Xanthomonas oryzae// pv. //oryzicola// suppresses rice immunity and promotes disease development. Mol. Plant Microbe Interact. 28: 869-880. DOI: [[https://doi.org/10.1094/MPMI-10-14-0314-R|10.1094/MPMI-10-14-0314-R]]
+Liu Y, Long J, Shen D, Song C (2016). //Xanthomonas oryzae// pv. //oryzae// requires H-NS-family protein XrvC to regulate virulence during rice infection. FEMS Microbiol. Lett. 363: fnw067. DOI: [[https://doi.org/10.1093/femsle/fnw067|10.1093/femsle/fnw067]]
 Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ<sub>PXO99</sub> in //Xanthomonas oryzae //pv. //oryzae//. Mol. Plant Microbe Interact. 23: 893-902. DOI: [[https://doi.org/10.1094/MPMI-23-7-0893|10.1094/MPMI-23-7-0893]]

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