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bacteria:t3e:xopy [2020/07/02 16:09] rkoebnik [References] |
bacteria:t3e:xopy [2020/07/09 12:13] (current) rkoebnik |
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====== XopY ====== | ====== XopY ====== | ||
- | Author: Irena Mačionienė\\ | + | Author: |
- | Internal reviewer: Lucas Morinière\\ | + | Internal reviewer: |
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
Class: XopY\\ | Class: XopY\\ | ||
Family: XopY\\ | Family: XopY\\ | ||
- | Prototype: | + | Prototype: |
- | RefSeq ID: [[https:// | + | RefSeq ID: [[https:// |
3D structure: Unknown | 3D structure: Unknown | ||
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=== How discovered? === | === How discovered? === | ||
- | XopY was discovered by screening the genome of //X. oryzae //pv. // | + | XopY was discovered by screening the genome of //X. oryzae //pv. // |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
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XopY from //X. oryzae //pv. //oryzae// posseses a PIP and ‐10 box in the promoter region (TTCGB‐N< | XopY from //X. oryzae //pv. //oryzae// posseses a PIP and ‐10 box in the promoter region (TTCGB‐N< | ||
- | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the // | + | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the // |
=== Phenotypes === | === Phenotypes === | ||
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XopY was demonstrated to target OsRLCK185. Expression of XopY in rice cells compromises OsRLCK185-mediated immune responses, which is consistent with the fact that Xoo1488 inhibits trans-phosphorylation of the activation domain of OsRLCK185 by OsCERK1. Interestingly, | XopY was demonstrated to target OsRLCK185. Expression of XopY in rice cells compromises OsRLCK185-mediated immune responses, which is consistent with the fact that Xoo1488 inhibits trans-phosphorylation of the activation domain of OsRLCK185 by OsCERK1. Interestingly, | ||
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===== Conservation ===== | ===== Conservation ===== | ||