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bacteria:t3e:xopaj [2020/08/14 00:18] jfpothier |
bacteria:t3e:xopaj [2022/06/22 13:55] rkoebnik |
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Authors: [[https:// | Authors: [[https:// | ||
Internal reviewer: [[https:// | Internal reviewer: [[https:// | ||
- | Expert reviewer: | + | Expert reviewer: |
Class: XopAJ\\ | Class: XopAJ\\ | ||
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=== How discovered? === | === How discovered? === | ||
- | Maize lines that contain the single dominant gene //Rxo1// exhibit a rapid hypersensitive response (HR) after infiltration with the rice bacterial streak pathogen // | + | Maize lines that contain the single dominant gene //Rxo1// exhibit a rapid hypersensitive response (HR) after infiltration with the nonhost |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
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=== Regulation === | === Regulation === | ||
- | No data available? :FIXME: | + | No data available. |
=== Phenotypes === | === Phenotypes === | ||
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* When introduced into //Xoo//, clones containing // | * When introduced into //Xoo//, clones containing // | ||
* // | * // | ||
- | * AvrRxo1 | + | * AvrRxo1 |
* Variants of AvrRxo1 were found to suppress the HR caused by the non-host resistance recognition of // | * Variants of AvrRxo1 were found to suppress the HR caused by the non-host resistance recognition of // | ||
- | * Among four // | + | * Among four // |
* The ATP/GTP binding site motif A and the NLS are required for both the avirulence activity and the suppression of non-host resistance (Liu //et al.//, 2014). | * The ATP/GTP binding site motif A and the NLS are required for both the avirulence activity and the suppression of non-host resistance (Liu //et al.//, 2014). | ||
- | * AvrRxo1 has a T4 polynucleotide kinase domain, and expression of AvrRxo1 suppresses bacterial growth in a manner dependent on the kinase motif (Han //et al.//, 2015). | + | * AvrRxo1 has a T4 polynucleotide kinase domain |
- | * The gene product of the adjacent gene, AvrRxo1-ORF2 aka Arc1, functions to suppress | + | * The gene product of the adjacent gene, AvrRxo1-ORF2 aka Arc1, suppresses |
- | * AvrRxo1 and its binding partner Arc1 function as a toxin-antitoxin system when expressed in // | + | * AvrRxo1 and its binding partner Arc1 function as a toxin-antitoxin system when expressed in // |
* XopAJ< | * XopAJ< | ||
- | * Mutation of the catalytic aspartic acid residue D< | + | * AvrRxo1 is a kinase that converts NAD to 3' |
* AvrRxo1 targets the cysteine protease RD21A, which is required for drought-induced immunity (Liu //et al.//, 2020). | * AvrRxo1 targets the cysteine protease RD21A, which is required for drought-induced immunity (Liu //et al.//, 2020). | ||
+ | * AvrRxo1, an ATP- dependent protease, enhances //Xoc// virulence and inhibits stomatal immunity by targeting and degrading rice OsPDX1 (pyridoxal phosphate synthase), thereby reducing vitamin B6 (VB6) levels in rice (Liu et al., 2022). | ||
=== Localization === | === Localization === | ||
- | Transient expression of // | + | Transient expression of // |
=== Enzymatic function === | === Enzymatic function === | ||
- | AvrRxo1 has a T4 polynucleotide kinase domain (Han //et al.//, 2015). | + | AvrRxo1 has a T4 polynucleotide kinase domain (Han //et al.//, 2015; Wu //et al//., 2015). |
- | AvrRxo1 is an authentic | + | AvrRxo1 is a phosphotransferase that produces two novel metabolites by phosphorylating nicotinamide/ |
- | AvrRxo1 | + | AvrRxo1 |
=== Interaction partners === | === Interaction partners === | ||
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The gene product of the adjacent gene, AvrRxo1-ORF2 aka Arc1, binds AvrRxo1, but binding is structurally different from typical effector-binding chaperones, in that it has a distinct fold containing a novel kinase-binding domain (Han //et al.//, 2015). | The gene product of the adjacent gene, AvrRxo1-ORF2 aka Arc1, binds AvrRxo1, but binding is structurally different from typical effector-binding chaperones, in that it has a distinct fold containing a novel kinase-binding domain (Han //et al.//, 2015). | ||
- | AvrRxo1 interacts with both the ubiquitin E3 ligase SINAT4 and the cysteine protease RD21A, enhancing | + | AvrRxo1 interacts with the // |
===== Conservation ===== | ===== Conservation ===== | ||
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AvrRxo1 appears to be widely conserved in Asian strains of // | AvrRxo1 appears to be widely conserved in Asian strains of // | ||
+ | |||
+ | AvrRxo1 is conserved in nearly all strains of //X. euvesicatoria//, | ||
=== In other plant pathogens/ | === In other plant pathogens/ | ||
- | Yes (// | + | Yes (// |
+ | |||
+ | Homologs of the // | ||
===== References ===== | ===== References ===== | ||
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Wonni I, Cottyn B, Detemmerman L, Dao S, Ouedraogo L, Sarra S, Tekete C, Poussier S, Corral R, Triplett L, Koita O, Koebnik R, Leach J, Szurek B, Maes M, Verdier V (2014). Analysis of // | Wonni I, Cottyn B, Detemmerman L, Dao S, Ouedraogo L, Sarra S, Tekete C, Poussier S, Corral R, Triplett L, Koita O, Koebnik R, Leach J, Szurek B, Maes M, Verdier V (2014). Analysis of // | ||
+ | |||
+ | Wu S (2015). Structural and functional characterization of a // | ||
Xie XW, Yu J, Xu JL, Zhou YL, Li ZK (2007). Introduction of a non-host gene // | Xie XW, Yu J, Xu JL, Zhou YL, Li ZK (2007). Introduction of a non-host gene // |