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bacteria:t3e:xopf [2020/07/03 09:52] rkoebnik |
bacteria:t3e:xopf [2020/09/10 18:29] rkoebnik [Conservation] |
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====== XopF ====== | ====== XopF ====== | ||
- | Author: Leonor Martins\\ | + | Author: |
Internal reviewer: [[https:// | Internal reviewer: [[https:// | ||
- | Expert reviewer: | + | Expert reviewer: |
Class: XopF\\ | Class: XopF\\ | ||
Family: XopF1, XopF2, XopF3\\ | Family: XopF1, XopF2, XopF3\\ | ||
- | Prototype: XopF (// | + | Prototype: XopF (// |
- | RefSeq ID: XopF1 [[https:// | + | RefSeq ID: XopF1_Xe |
+ | Synonym: Hpa4\\ | ||
3D structure: Unknown | 3D structure: Unknown | ||
===== Biological function ===== | ===== Biological function ===== | ||
- | How discovered? | + | === How discovered? |
- | XopF1 and XopF2 were identified | + | XopF1 and XopF2 were identified |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | //Xcv// XopF1:: | + | Type III-dependent secretion of XopF1 and XopF2 was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control |
+ | |||
+ | Fragments of the //xopF1// gene are located within the //hrp// cluster of many // | ||
+ | |||
+ | XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an Xcv chaperone (Roden //et al//., 2004). | ||
=== Regulation === | === Regulation === | ||
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=== Phenotypes === | === Phenotypes === | ||
- | To study the possible virulence function of the putative //xopF1// operon encoding HpaD, HpaI, and XopF1 these three genes were deleted from the genome of //X. campestris// | + | * Roden et al. did not find significant growth defects of a // |
+ | * To study the possible virulence function of the putative // | ||
+ | * Later, // | ||
+ | * Additionally, | ||
+ | * // | ||
=== Localization === | === Localization === | ||
- | XopF2 localizes in the Golgi apparatus, while XopF1 has been found in cytoplasm (Popov //et al//., 2016) and plasma membrane (Mondal //et al//., 2016). XopF1 is encoded within //hrp// region, between //hpaB// and //hrpF//, while XopF2 is encoded elsewhere in the bacterial chromosome (Roden //et al//., 2004; Büttner //et al//., 2007). | + | XopF2 localizes in the Golgi apparatus, while XopF1 has been found in cytoplasm (Popov //et al//., 2016) and plasma membrane (Mondal //et al//., 2016). XopF1 is encoded within // |
=== Enzymatic function === | === Enzymatic function === | ||
Line 36: | Line 46: | ||
=== Interaction partners === | === Interaction partners === | ||
- | XopF1 secretion and translocation is T3S-dependent, HpaH, HpaC and T3S chaperone HpaB are required for efficient secretion XopF1 (Büttner //et al//., 2006, 2007). | + | XopF1 secretion and translocation is T3SS-dependent; HpaH, HpaC and T3S chaperone HpaB are required for efficient secretion XopF1 (Büttner //et al//., 2006, 2007). |
===== Conservation ===== | ===== Conservation ===== | ||
=== In xanthomonads === | === In xanthomonads === | ||
- | Yes (//e.g.//, //X. oryzae//, //X. euvesicatoria//, //X. translucens//, //X. bromi//, //X. citri, X. arboricola, X. campestris pv. musacearum//). | + | Yes (//e.g.//, //X. arboricola, X. bromi//, //X. citri, X. oryzae// pv.// oryzae//, //X. euvesicatoria//, //X. translucens//, //X. vasicola//). Since the G+C content of the //xopF1// gene is similar to that of the //Xcv// //hrp// gene cluster, it may be a member of a “core” group of // |
=== In other plant pathogens/ | === In other plant pathogens/ | ||
Line 48: | Line 59: | ||
===== References ===== | ===== References ===== | ||
- | Büttner D, Lorenz C, Weber E, Bonas U (2006). Targeting of two effector protein classes to the type III secretion system by a HpaC- and HpaB-dependent protein complex from // | + | Büttner D, Lorenz C, Weber E, Bonas U (2006). Targeting of two effector protein classes to the type III secretion system by a HpaC- and HpaB-dependent protein complex from // |
+ | |||
+ | Büttner D, Noël L, Stuttmann J, Bonas U (2007). Characterization of the nonconserved // | ||
+ | |||
+ | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // | ||
- | Büttner D, Noël L, Stuttmann | + | Liu Y, Long J, Shen D, Song C (2016). // |
- | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // | + | Mondal K K, Verma G, Manju, Junaid |
- | Liu Y, Long J, Shen D, Song C (2016). // | + | Popov G, Fraiture M, Brunner F, Sessa G (2016). |
- | Mondal K K, Verma G, Manju, Junaid A, Mani C (2016). Rice pathogen | + | Roden J, Belt B, Ross J, Tachibana T, Vargas J, Mudgett M (2004). A genetic screen to isolate type III effectors translocated into pepper cells during |
- | Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple // | + | ===== Further reading ===== |
- | Roden J, Belt B, Ross J, Tachibana T, Vargas J, Mudgett M (2004). A genetic screen to isolate type III effectors translocated into pepper cells during | + | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of Xanthomonas campestris pv. //vesicatoria// type III effectors in yeast affects cell growth and viability. Mol. Plant Microbe Interact. 24: 305-314. DOI: [[https://doi.org/10.1094/MPMI-09-10-0196|10.1094/MPMI-09-10-0196]] |