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bacteria:t3e:xopf [2020/07/02 16:30] rkoebnik [Biological function] |
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- | ====== XopF ====== | ||
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- | Author: Maria Leonor Martins\\ | ||
- | Internal reviewer: Jaime Cubero\\ | ||
- | Expert reviewer: FIXME | ||
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- | Class: XopF\\ | ||
- | Family: XopF1, XopF2, XopF3\\ | ||
- | Prototype: XopF (// | ||
- | RefSeq ID: XopF1 [[https:// | ||
- | 3D structure: Unknown | ||
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- | ===== Biological function ===== | ||
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- | How discovered? | ||
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- | XopF1 and XopF2 were identified by a genetic screen using AvrBs2 as a reporter protein (Roden //et al//., 2004). | ||
- | === (Experimental) evidence for being a T3E === | ||
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- | //Xcv// XopF1:: | ||
- | === Regulation === | ||
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- | RT-PCR analysis revealed //xopF1// is regulated by //hrpG// and //hrpX// and that //xopF1//, //hpaD//, //hpaI// belong to the same operon. Upstream there is a PIP box which provides binding site for HrpX (Büttner //et al//., 2007). | ||
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- | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopF//, were significantly reduced in the // | ||
- | === Phenotypes === | ||
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- | To study the possible virulence function of the putative //xopF1// operon encoding HpaD, HpaI, and XopF1 these three genes were deleted from the genome of //X. campestris// | ||
- | === Localization === | ||
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- | XopF2 localizes in the Golgi apparatus, while XopF1 has been found in cytoplasm (Popov //et al//., 2016) and plasma membrane (Mondal //et al//., 2016). XopF1 is encoded within //hrp// region, between //hpaB// and //hrpF//, while XopF2 is encoded elsewhere in the bacterial chromosome (Roden //et al//., 2004; Büttner //et al//., 2007). | ||
- | === Enzymatic function === | ||
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- | Unknown. | ||
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- | === Interaction partners === | ||
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- | XopF1 secretion and translocation is T3S-dependent, | ||
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- | ===== Conservation ===== | ||
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- | === In xanthomonads === | ||
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- | Yes (//e.g.//, //X. oryzae//, //X. euvesicatoria//, | ||
- | === In other plant pathogens/ | ||
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- | Unknown. | ||
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- | ===== References ===== | ||
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- | Büttner D, Lorenz C, Weber E, Bonas U (2006). Targeting of two effector protein classes to the type III secretion system by a HpaC- and HpaB-dependent protein complex from // | ||
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- | Büttner D, Noël L, Stuttmann J, Bonas U (2007). Characterization of the nonconserved // | ||
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- | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // | ||
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- | Liu Y, Long J, Shen D, Song C (2016). // | ||
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- | Mondal K K, Verma G, Manju, Junaid A, Mani C (2016). Rice pathogen // | ||
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- | Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple // | ||
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- | Roden J, Belt B, Ross J, Tachibana T, Vargas J, Mudgett M (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | ||