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bacteria:t3e:xopq [2020/07/03 18:56] rkoebnik |
bacteria:t3e:xopq [2020/07/17 10:13] rkoebnik [References] |
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Class: XopQ\\ | Class: XopQ\\ | ||
Family: XopQ\\ | Family: XopQ\\ | ||
- | Prototype: XCV4438: Xanthomonas outer protein Q from // | + | Prototype: XCV4438 |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
3D structure: [[https:// | 3D structure: [[https:// | ||
+ | |||
===== Biological function ===== | ===== Biological function ===== | ||
=== How discovered? === | === How discovered? === | ||
- | XopQ was identified in a genetic screen, using a Tn// | + | XopQ was identified in a genetic screen, using a Tn// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
Line 32: | Line 33: | ||
* Mutations of two potential active site residues, D116 and Y279, resulted in // | * Mutations of two potential active site residues, D116 and Y279, resulted in // | ||
* Compatibility studies with //X. euvesicatoria// | * Compatibility studies with //X. euvesicatoria// | ||
- | * XopQ mediated cell death suppression in //N. benthamiana// | + | |
+ | * Transient co-expression of XopQ::GFP and XopS::GFP in //N. benthamiana// | ||
+ | * XopQ suppressed cell death reactions in //N. benthamiana// | ||
+ | | ||
* A Δ// | * A Δ// | ||
* A reverse genetics screen identified Recognition of XopQ 1 (Roq1), a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, which mediates XopQ recognition in //N. benthamiana// | * A reverse genetics screen identified Recognition of XopQ 1 (Roq1), a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, which mediates XopQ recognition in //N. benthamiana// | ||
* Roq1 is able to recognize XopQ alleles from various // | * Roq1 is able to recognize XopQ alleles from various // | ||
+ | * The coiled-coil NLR protein N requirement gene 1 (NRG) interacts with EDS1 and acts downstream of Roq1 and EDS1 to mediate XopQ/ | ||
+ | * Roq1 is also involved in the recognition of RipB, the homolog of XopQ in //Ralstonia solanacearum//: | ||
* Effectors that interact with 14–3–3 proteins may provide plant-pathogenic bacteria with the ability to modulate PTI as well as ETI. Suppression of immune responses induced by a // | * Effectors that interact with 14–3–3 proteins may provide plant-pathogenic bacteria with the ability to modulate PTI as well as ETI. Suppression of immune responses induced by a // | ||
+ | * Roq1 was found to confer immunity to // | ||
+ | * Strong resistance to // | ||
=== Localization === | === Localization === | ||
Line 52: | Line 60: | ||
=== Interaction partners === | === Interaction partners === | ||
- | Using protein-protein interaction studies in yeast and in planta, XopQ< | + | Using protein-protein interaction studies in yeast and in planta, XopQ< |
+ | |||
+ | Bimolecular fluorescence complementation assays upon transient expression in //N. benthamiana// | ||
Roq1, a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, was found to co-immunoprecipitate with XopQ, suggesting a physical association between the two proteins (Schultink //et al.//, 2017). | Roq1, a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, was found to co-immunoprecipitate with XopQ, suggesting a physical association between the two proteins (Schultink //et al.//, 2017). | ||
Line 62: | Line 72: | ||
=== In xanthomonads === | === In xanthomonads === | ||
- | XopQ is a widely conserved across // | + | XopQ is a widely conserved across // |
=== In other plant pathogens/ | === In other plant pathogens/ | ||
- | XopQ shares homology with the //Ralstonia solanacearum// | + | XopQ shares homology with the //Ralstonia solanacearum// |
===== References ===== | ===== References ===== | ||
- | Adlung N, Bonas U (2017). Dissecting virulence function from recognition: | + | Adlung N (2016). Charakterisierung der Avirulenzaktivität von XopQ und Identifizierung möglicher Interaktoren von XopL aus //Xanthomonas campestris// pv. // |
- | Adlung N, Prochaska H, Thieme S, Banik A, Blüher D, John P, Nagel O, Schulze S, Gantner J, Delker C, Stuttmann J, Bonas U (2016). Non-host resistance induced by the //Xanthomonas// | + | Adlung N, Bonas U (2017). Dissecting virulence function from recognition: |
- | Büttner | + | Adlung N, Prochaska H, Thieme S, Banik A, Blüher |
- | Deb S, Gupta MK, Patel HK, Sonti RV (2019). // | + | Büttner D, Bonas U (2010). Regulation and secretion of // |
- | Furutani A,Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification | + | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction |
- | Gupta MK, Nathawat R, Sinha D, Haque AS, Sankaranarayanan R, Sonti RV (2015). Mutations in the predicted active site of // | + | Deb S, Gupta MK, Patel HK, Sonti RV (2019). // |
+ | |||
+ | Dubrow Z, Sunitha S, Kim JG, Aakre CD, Girija AM, Sobol G, Teper D, Chen YC, Ozbaki-Yagan N, Vance H, Sessa G, Mudgett MB (2018). Tomato 14-3-3 proteins are required for //Xv3// disease resistance and interact with a subset of // | ||
+ | |||
+ | Furutani A,Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // | ||
+ | |||
+ | Gupta MK, Nathawat R, Sinha D, Haque AS, Sankaranarayanan R, Sonti RV (2015). Mutations in the predicted active site of // | ||
Hajri A, Brin C, Hunault G, Lardeux F, Lemaire C, Manceau C, Boureau T, Poussier S (2009). A " | Hajri A, Brin C, Hunault G, Lardeux F, Lemaire C, Manceau C, Boureau T, Poussier S (2009). A " | ||
- | Jiang W, Jiang B, Xu R, Huang J, Wei H, Jiang GF, Cen WJ, Liu J, Ge YY, Li GH, Su LL, Hang XH, Tang DJ, Lu GT, Feng JX, He YQ, Tang JL (2009). Identification of six type III effector genes with the PIP box in // | + | Jiang W, Jiang B, Xu R, Huang J, Wei H, Jiang GF, Cen WJ, Liu J, Ge YY, Li GH, Su LL, Hang XH, Tang DJ, Lu GT, Feng JX, He YQ, Tang JL (2009). Identification of six type III effector genes with the PIP box in // |
+ | |||
+ | Liu Y, Long J, Shen D, Song C (2016). // | ||
+ | |||
+ | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | ||
+ | |||
+ | Nakano M, Mukaihara T (2019). The type III effector RipB from //Ralstonia solanacearum// | ||
- | Liu Y, Long J, Shen D, Song C (2016). //Xanthomonas oryzae// pv. // | + | Qi T, Seong K, Thomazella DPT, Kim JR, Pham J, Seo E, Cho MJ, Schultink A, Staskawicz BJ (2018). NRG1 functions downstream of EDS1 to regulate TIR-NLR-mediated plant immunity in //Nicotiana benthamiana//. Proc. Natl. Acad. Sci. USA 115: E10979-E10987. DOI: [[https:// |
- | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors | + | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate |
- | Roden JA, Belt B, Ross JB, Tachibana | + | Schultink A, Qi T, Lee A, Steinbrenner AD, Staskawicz B (2017). Roq1 mediates recognition of the // |
- | Schultink A, Qi T, Lee A, Steinbrenner AD, Staskawicz | + | Schwartz AR, Potnis N, Timilsina S, Wilson M, Patané J, Martins J Jr, Minsavage GV, Dahlbeck D, Akhunova |
- | Schwartz AR, Potnis N, Timilsina S, Wilson M, Patané J, Martins J Jr, Minsavage GV, Dahlbeck D, Akhunova | + | Sinha D, Gupta MK, Patel HK, Ranjan |
- | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // | + | Teper D, SalomonD, Sunitha S, Kim JG, Mudgett MB, Sessa G. (2014). // |
- | Teper D, SalomonD, Sunitha S, Kim JG, Mudgett MB, Sessa G. (2014). // | + | Thomas NC, Hendrich CG, Gill US, Allen C, Hutton SF, Schultink A (2020). The immune receptor Roq1 confers resistance to the bacterial pathogens |
Yu S, Hwang I, Rhee S (2013). Crystal structure of the effector protein XOO4466 from // | Yu S, Hwang I, Rhee S (2013). Crystal structure of the effector protein XOO4466 from // | ||
- | Yu S, Hwang I, Rhee S (2014). The crystal structure of type III effector protein XopQ from // | + | Yu S, Hwang I, Rhee S (2014). The crystal structure of type III effector protein XopQ from // |