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bacteria:t3e:xopq [2020/07/06 12:47] rkoebnik |
bacteria:t3e:xopq [2020/07/17 10:13] rkoebnik [References] |
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Class: XopQ\\ | Class: XopQ\\ | ||
Family: XopQ\\ | Family: XopQ\\ | ||
- | Prototype: XCV4438: Xanthomonas outer protein Q from // | + | Prototype: XCV4438 |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
3D structure: [[https:// | 3D structure: [[https:// | ||
+ | |||
===== Biological function ===== | ===== Biological function ===== | ||
=== How discovered? === | === How discovered? === | ||
- | XopQ was identified in a genetic screen, using a Tn// | + | XopQ was identified in a genetic screen, using a Tn// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
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* Mutations of two potential active site residues, D116 and Y279, resulted in // | * Mutations of two potential active site residues, D116 and Y279, resulted in // | ||
* Compatibility studies with //X. euvesicatoria// | * Compatibility studies with //X. euvesicatoria// | ||
- | * XopQ mediated cell death suppression in //N. benthamiana// | + | |
+ | * Transient co-expression of XopQ::GFP and XopS::GFP in //N. benthamiana// | ||
+ | * XopQ suppressed cell death reactions in //N. benthamiana// | ||
+ | | ||
* A Δ// | * A Δ// | ||
* A reverse genetics screen identified Recognition of XopQ 1 (Roq1), a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, which mediates XopQ recognition in //N. benthamiana// | * A reverse genetics screen identified Recognition of XopQ 1 (Roq1), a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, which mediates XopQ recognition in //N. benthamiana// | ||
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* Roq1 is also involved in the recognition of RipB, the homolog of XopQ in //Ralstonia solanacearum//: | * Roq1 is also involved in the recognition of RipB, the homolog of XopQ in //Ralstonia solanacearum//: | ||
* Effectors that interact with 14–3–3 proteins may provide plant-pathogenic bacteria with the ability to modulate PTI as well as ETI. Suppression of immune responses induced by a // | * Effectors that interact with 14–3–3 proteins may provide plant-pathogenic bacteria with the ability to modulate PTI as well as ETI. Suppression of immune responses induced by a // | ||
+ | * Roq1 was found to confer immunity to // | ||
+ | * Strong resistance to // | ||
=== Localization === | === Localization === | ||
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Using protein-protein interaction studies in yeast and in planta, XopQ< | Using protein-protein interaction studies in yeast and in planta, XopQ< | ||
+ | |||
+ | Bimolecular fluorescence complementation assays upon transient expression in //N. benthamiana// | ||
Roq1, a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, was found to co-immunoprecipitate with XopQ, suggesting a physical association between the two proteins (Schultink //et al.//, 2017). | Roq1, a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, was found to co-immunoprecipitate with XopQ, suggesting a physical association between the two proteins (Schultink //et al.//, 2017). | ||
Line 64: | Line 72: | ||
=== In xanthomonads === | === In xanthomonads === | ||
- | XopQ is a widely conserved across // | + | XopQ is a widely conserved across // |
=== In other plant pathogens/ | === In other plant pathogens/ | ||
- | XopQ shares homology with the //Ralstonia solanacearum// | + | XopQ shares homology with the //Ralstonia solanacearum// |
===== References ===== | ===== References ===== | ||
- | Adlung N (2016). Charakterisierung der Avirulenzaktivität von XopQ und Identifizierung möglicher Interaktoren von XopL aus // | + | Adlung N (2016). Charakterisierung der Avirulenzaktivität von XopQ und Identifizierung möglicher Interaktoren von XopL aus // |
+ | |||
+ | Adlung N, Bonas U (2017). Dissecting virulence function from recognition: | ||
- | Adlung N, Bonas U (2017). Dissecting virulence function from recognition: | + | Adlung N, Prochaska H, Thieme S, Banik A, Blüher D, John P, Nagel O, Schulze S, Gantner J, Delker C, Stuttmann J, Bonas U (2016). Non-host resistance induced by the //Xanthomonas// effector |
- | Adlung N, Prochaska H, Thieme S, Banik A, Blüher | + | Büttner |
- | Büttner D, Bonas U (2010). Regulation and secretion | + | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction |
- | Deb S, Gupta MK, Patel HK, Sonti RV (2019). // | + | Deb S, Gupta MK, Patel HK, Sonti RV (2019). // |
- | Dubrow Z, Sunitha S, Kim JG, Aakre CD, Girija AM, Sobol G, Teper D, Chen YC, Ozbaki-Yagan N, Vance H, Sessa G, Mudgett MB (2018). Tomato 14-3-3 proteins are required for // | + | Dubrow Z, Sunitha S, Kim JG, Aakre CD, Girija AM, Sobol G, Teper D, Chen YC, Ozbaki-Yagan N, Vance H, Sessa G, Mudgett MB (2018). Tomato 14-3-3 proteins are required for //Xv3// disease resistance and interact with a subset of // |
- | Furutani A,Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // | + | Furutani A,Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // |
- | Gupta MK, Nathawat R, Sinha D, Haque AS, Sankaranarayanan R, Sonti RV (2015). Mutations in the predicted active site of // | + | Gupta MK, Nathawat R, Sinha D, Haque AS, Sankaranarayanan R, Sonti RV (2015). Mutations in the predicted active site of // |
Hajri A, Brin C, Hunault G, Lardeux F, Lemaire C, Manceau C, Boureau T, Poussier S (2009). A " | Hajri A, Brin C, Hunault G, Lardeux F, Lemaire C, Manceau C, Boureau T, Poussier S (2009). A " | ||
- | Jiang W, Jiang B, Xu R, Huang J, Wei H, Jiang GF, Cen WJ, Liu J, Ge YY, Li GH, Su LL, Hang XH, Tang DJ, Lu GT, Feng JX, He YQ, Tang JL (2009). Identification of six type III effector genes with the PIP box in // | + | Jiang W, Jiang B, Xu R, Huang J, Wei H, Jiang GF, Cen WJ, Liu J, Ge YY, Li GH, Su LL, Hang XH, Tang DJ, Lu GT, Feng JX, He YQ, Tang JL (2009). Identification of six type III effector genes with the PIP box in // |
- | Liu Y, Long J, Shen D, Song C (2016). // | + | Liu Y, Long J, Shen D, Song C (2016). // |
- | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | + | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // |
- | Nakano M, Mukaihara T (2019). The type III effector RipB from //Ralstonia solanacearum// | + | Nakano M, Mukaihara T (2019). The type III effector RipB from //Ralstonia solanacearum// |
Qi T, Seong K, Thomazella DPT, Kim JR, Pham J, Seo E, Cho MJ, Schultink A, Staskawicz BJ (2018). NRG1 functions downstream of EDS1 to regulate TIR-NLR-mediated plant immunity in //Nicotiana benthamiana// | Qi T, Seong K, Thomazella DPT, Kim JR, Pham J, Seo E, Cho MJ, Schultink A, Staskawicz BJ (2018). NRG1 functions downstream of EDS1 to regulate TIR-NLR-mediated plant immunity in //Nicotiana benthamiana// | ||
- | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | + | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // |
- | Schultink A, Qi T, Lee A, Steinbrenner AD, Staskawicz B (2017). Roq1 mediates recognition of the // | + | Schultink A, Qi T, Lee A, Steinbrenner AD, Staskawicz B (2017). Roq1 mediates recognition of the // |
- | Schwartz AR, Potnis N, Timilsina S, Wilson M, Patané J, Martins J Jr, Minsavage GV, Dahlbeck D, Akhunova A, Almeida N, Vallad GE, Barak JD, White FF, Miller SA, Ritchie D, Goss E, Bart RS, Setubal JC, Jones JB, Staskawicz BJ (2015). Phylogenomics of // | + | Schwartz AR, Potnis N, Timilsina S, Wilson M, Patané J, Martins J Jr, Minsavage GV, Dahlbeck D, Akhunova A, Almeida N, Vallad GE, Barak JD, White FF, Miller SA, Ritchie D, Goss E, Bart RS, Setubal JC, Jones JB, Staskawicz BJ (2015). Phylogenomics of // |
- | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // | + | Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of // |
- | Teper D, SalomonD, Sunitha S, Kim JG, Mudgett MB, Sessa G. (2014). // | + | Teper D, SalomonD, Sunitha S, Kim JG, Mudgett MB, Sessa G. (2014). // |
- | Thomas NC, Hendrich CG, Gill US, Allen C, Hutton SF, Schultink A (2020). The immune receptor Roq1 confers resistance to the bacterial pathogens // | + | Thomas NC, Hendrich CG, Gill US, Allen C, Hutton SF, Schultink A (2020). The immune receptor Roq1 confers resistance to the bacterial pathogens // |
Yu S, Hwang I, Rhee S (2013). Crystal structure of the effector protein XOO4466 from // | Yu S, Hwang I, Rhee S (2013). Crystal structure of the effector protein XOO4466 from // | ||
- | Yu S, Hwang I, Rhee S (2014). The crystal structure of type III effector protein XopQ from // | + | Yu S, Hwang I, Rhee S (2014). The crystal structure of type III effector protein XopQ from // |