This shows you the differences between two versions of the page.
Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
bacteria:t3e:xopq [2020/07/06 13:16] rkoebnik [Biological function] |
bacteria:t3e:xopq [2020/07/17 10:25] rkoebnik [Biological function] |
||
---|---|---|---|
Line 7: | Line 7: | ||
Class: XopQ\\ | Class: XopQ\\ | ||
Family: XopQ\\ | Family: XopQ\\ | ||
- | Prototype: XCV4438: Xanthomonas outer protein Q from // | + | Prototype: XCV4438 |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
3D structure: [[https:// | 3D structure: [[https:// | ||
+ | |||
===== Biological function ===== | ===== Biological function ===== | ||
=== How discovered? === | === How discovered? === | ||
- | XopQ was identified in a genetic screen, using a Tn// | + | XopQ was identified in a genetic screen, using a Tn// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
Line 33: | Line 34: | ||
* Compatibility studies with //X. euvesicatoria// | * Compatibility studies with //X. euvesicatoria// | ||
* The avirulence activity of XopQ derivatives did not correlate with macroscopically visible plant reactions upon transient expression in //N. benthamiana// | * The avirulence activity of XopQ derivatives did not correlate with macroscopically visible plant reactions upon transient expression in //N. benthamiana// | ||
+ | * Transient co-expression of XopQ::GFP and XopS::GFP in //N. benthamiana// | ||
* XopQ suppressed cell death reactions in //N. benthamiana// | * XopQ suppressed cell death reactions in //N. benthamiana// | ||
- | * XopQ-mediated cell death suppression in //N. benthamiana// | + | * XopQ-mediated cell death suppression in //N. benthamiana// |
* A Δ// | * A Δ// | ||
* A reverse genetics screen identified Recognition of XopQ 1 (Roq1), a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, which mediates XopQ recognition in //N. benthamiana// | * A reverse genetics screen identified Recognition of XopQ 1 (Roq1), a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, which mediates XopQ recognition in //N. benthamiana// | ||
Line 59: | Line 61: | ||
Using protein-protein interaction studies in yeast and in planta, XopQ< | Using protein-protein interaction studies in yeast and in planta, XopQ< | ||
+ | |||
+ | Bimolecular fluorescence complementation assays upon transient expression in //N. benthamiana// | ||
Roq1, a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, was found to co-immunoprecipitate with XopQ, suggesting a physical association between the two proteins (Schultink //et al.//, 2017). | Roq1, a nucleotide-binding leucine-rich repeat (NLR) protein with a Toll-like interleukin-1 receptor (TIR) domain, was found to co-immunoprecipitate with XopQ, suggesting a physical association between the two proteins (Schultink //et al.//, 2017). | ||
- | XopQ< | + | XopQ< |
+ | |||
+ | Yeast two-hybrid, bimolecular fluorescence complementation (BiFC) and co-IP assays indicated that XopQ and XopX interact with each other (Deb et al., 2020). | ||
===== Conservation ===== | ===== Conservation ===== | ||
Line 83: | Line 89: | ||
Büttner D, Bonas U (2010). Regulation and secretion of // | Büttner D, Bonas U (2010). Regulation and secretion of // | ||
+ | |||
+ | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // | ||
Deb S, Gupta MK, Patel HK, Sonti RV (2019). // | Deb S, Gupta MK, Patel HK, Sonti RV (2019). // |