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bacteria:t3e:xopz [2020/07/09 10:38] rkoebnik [References] |
bacteria:t3e:xopz [2020/08/02 18:48] jfpothier |
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Author: Marlène Lachaux\\ | Author: Marlène Lachaux\\ | ||
- | Internal reviewer: | + | Internal reviewer: |
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
Class: XopZ\\ | Class: XopZ\\ | ||
Family: XopZ\\ | Family: XopZ\\ | ||
- | Prototype: XopZ (// | + | Prototype: XopZ (// |
- | RefSeq ID: 1,414-amino-acid protein 3D structure: The N-terminus of XopZ< | + | RefSeq ID: 1,414 amino-acid protein |
+ | 3D structure: | ||
===== Biological function ===== | ===== Biological function ===== | ||
=== How discovered? === | === How discovered? === | ||
- | In 2009, generation of PXO99 mutants for 18 non-TAL | + | In 2009, generation of mutants for 18 non-TAL |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | With a PIP box upstream of the predicted translation start site //XopZ< | + | With a PIP box 58 bp upstream of the predicted translation start site, //xopZ< |
=== Regulation === | === Regulation === | ||
- | The //XopZ// gene was shown to be expressed in a // | + | The //xopZ// gene was shown to be expressed in a // |
qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopZ//, were significantly reduced in the // | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopZ//, were significantly reduced in the // | ||
=== Phenotypes === | === Phenotypes === | ||
- | PXO99< | + | PXO99< |
=== Localization === | === Localization === | ||
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=== Enzymatic function === | === Enzymatic function === | ||
- | XopZ< | + | XopZ< |
=== Interaction partners === | === Interaction partners === | ||
XopZ interacts with a putative host E3 ubiquitin ligase protein PBP (s-ribonuclease) //in vitro// and //in vivo//. Regions containing 193 aa - 225 aa of PBP is required for interacting with XopZ. PBP is a negative regulator of host immune response based on the disease phenotype in PBP-knockout rice plants. C1A directly interacts and strongly degrades PBP through its cysteine protease activity, leading to a homeostatic state of PBP in plant cells (Zhou //et al//., 2015). | XopZ interacts with a putative host E3 ubiquitin ligase protein PBP (s-ribonuclease) //in vitro// and //in vivo//. Regions containing 193 aa - 225 aa of PBP is required for interacting with XopZ. PBP is a negative regulator of host immune response based on the disease phenotype in PBP-knockout rice plants. C1A directly interacts and strongly degrades PBP through its cysteine protease activity, leading to a homeostatic state of PBP in plant cells (Zhou //et al//., 2015). | ||
- | |||
===== Conservation ===== | ===== Conservation ===== | ||
=== In xanthomonads === | === In xanthomonads === | ||
- | Yes, is found to be conserved in all // | + | Yes, found to be conserved in all // |
=== In other plant pathogens/ | === In other plant pathogens/ | ||
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Liu Y, Long J, Shen D, Song C (2016). // | Liu Y, Long J, Shen D, Song C (2016). // | ||
+ | |||
+ | Long J, Song C, Yan F, Zhou J, Zhou H, Yang B (2018). Non-TAL effectors from // | ||
Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // | Medina CA, Reyes PA, Trujillo CA, Gonzalez JL, Bejarano DA, Montenegro NA, Jacobs JM, Joe A, Restrepo S, Alfano JR, Bernal A (2018). The role of type III effectors from // |