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bacteria:t3e:avrbs2 [2020/07/13 14:08] rkoebnik [Biological function] |
bacteria:t3e:avrbs2 [2020/07/17 10:32] (current) rkoebnik [Biological function] |
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Prototype: AvrBs2 (// | Prototype: AvrBs2 (// | ||
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
- | Synonym: | + | Synonym: |
3D structure: Unknown | 3D structure: Unknown | ||
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Indirectly – the pathovars that induced // | Indirectly – the pathovars that induced // | ||
- | |||
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | AvrBs2 fused to the calmodulin-activated adenylate cyclase domain was shown to translocate into plant cells (cytosol), detected through rise of cAMP levels inside the plant tissue. The // | + | AvrBs2 fused to the calmodulin-activated adenylate cyclase domain was shown to translocate into plant cells (cytosol), detected through rise of cAMP levels inside the plant tissue. The //hrpF// < |
=== Regulation === | === Regulation === | ||
- | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes // | + | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //avrBs2//, were significantly reduced in the // |
- | + | ||
- | Transcriptome analysis (RNA-seq) and qRT-PCR have shown that // | + | |
+ | Transcriptome analysis (RNA-seq) and qRT-PCR have shown that //avrBs2// gene expression is downregulated in a //X. citri// pv. //citri// Δ//phoP// mutant, indicating that PhoP is a positive regulator of //avrBs2// expression (Wei //et al//., 2019). | ||
=== Phenotypes === | === Phenotypes === | ||
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* Induced expression of AvrBs2 in transgenic cell cultures was shown to dramatically suppress flg22-induced and chitin-induced immune responses, such as ROS burst and PR gene expression (Li //et al//., 2015). | * Induced expression of AvrBs2 in transgenic cell cultures was shown to dramatically suppress flg22-induced and chitin-induced immune responses, such as ROS burst and PR gene expression (Li //et al//., 2015). | ||
* A ∆// | * A ∆// | ||
+ | * // | ||
=== Localization === | === Localization === | ||
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Coplin DL (1989). Plasmids and their role in the evolution of plant pathogenic bacteria. Ann. Rev. Phytopathol. 27: 187-212. DOI: [[https:// | Coplin DL (1989). Plasmids and their role in the evolution of plant pathogenic bacteria. Ann. Rev. Phytopathol. 27: 187-212. DOI: [[https:// | ||
+ | |||
+ | Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the // | ||
Gassmann W, Dahlbeck D, Chesnokova O, Minsavage GV, Jones JB, Staskawicz BJ (2000). Molecular evolution of virulence in natural field strains of // | Gassmann W, Dahlbeck D, Chesnokova O, Minsavage GV, Jones JB, Staskawicz BJ (2000). Molecular evolution of virulence in natural field strains of // |