User Tools

Site Tools


Sidebar

Learn about COST & EuroXanth


Bacterial virulence factors

Plant resistance genes


Molecular Diagnosis and Diversity for Regulated Xanthomonas


List of contributors


Disclaimer

Privacy policy


DokuWiki Syntax


This DokuWiki is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology)


Follow EuroXanth on Twitter, ResearchGate or Scoop.it!

bacteria:t3e:xopad

XopAD

Author: David J. Studholme
Internal reviewer: Laurent D. Noël
Expert reviewer: FIXME

Class: XopAD
Family: XopAD
Prototype: XopAD (Xanthomonas euvesicatoria pv. euvesicatoria, ex Xanthomonas campestris pv. vescicatoria; strain 85-10)
RefSeq ID: not found in RefSeq. GenBank accession: CAJ26046.1 (614 aa)
3D structure: Unknown

Biological function

How discovered?

XopAD was discovered using a machine-learning approach (Teper et al., 2016).

(Experimental) evidence for being a T3E

XopAD fused to the AvrBs2 reporter domain, was shown to translocate into plant cells in an hrpF-dependent manner.

Regulation

No PIP box was found in the promoter region of xopAD in X. euvesicatoria strain 85-10 (Teper et al., 2016).

qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes hrpG and hrpX), including xopAD, were significantly reduced in the Xanthomonas oryzae pv. oryzae ΔxrvC mutant compared with those in the wild-type strain PXO99A (Liu et al., 2016).

Phenotypes

Deletion of xopAD does not alter X. citri pv. citri (Xci) pathogenicity (Escalon et al., 2013).

Localization

Unknown.

Enzymatic function

Not known. However, the 614 amino acid protein consists of multiple armadillo repeats of semi-conserved 42 amino acids. The C-terminal domain, which is absent in Xcv 85-10 XopAD but present in the ~2880 amino acid homologues (see below), encodes a putative RelA-like nucleotidyltransferase domain (Teper et al., 2016).

Interaction partners

Not known.

Conservation

In xanthomonads

Yes. XopAD has homologues encoded in the genomes of most Xanthomonas species (Teper et al., 2016), including X. axonopodis (Harrison & Studholme, 2014), X. vasicola (Studholme et al., 2010; Wasukira et al., 2012), X. nasturtii (Vicente et al., 2010), X. citri (Escalon et al., 2013). In this respect, Xanthomonas campestris appears to be an exception. Escalon and colleagues state “The analysis of xopAD and xopAG suggested horizontal transfer between X. citri pv. bilvae, another citrus pathogen, and some Xci strains” (Escalon et al., 2013). The prototype sequence from X. euvesicatoria strain 85-10 (Teper et al., 2016) is 614 amino acids in length and marked in GenBank as a fragment. Homologues in other genomes of this species range from 2840 (RefSeq: WP_046939801.1) to 2885 (RefSeq: WP_033837371.1) amino acids in length and the authors of the prototype study state: “we hypothesize that the ORFs annotated as XCV1197 (XopAV) and XCV1198, and XCV4315 (XopAD), XCV4314 and XCV4313, were originally two complete ORFs that were later truncated by the introduction of early stop codons” (Teper et al., 2016). Therefore, the full-length homologues found in other genomes might not be functionally equivalent to the prototype XopAD. The introduction of early stop codons is explained by presence of an ISXac5-related insertion sequence (Escalon et al., 2013).

In other plant pathogens/symbionts

Yes. XopAD is homologous to members of the RipS1 family of effectors in Ralstonia solanacearum (Peeters et al., 2013).

References

Escalon A, Javegny S, Vernière C, Noël LD, Vital K, Poussier S, Hajri A, Boureau T, Pruvost O, Arlat M, Gagnevin L (2013). Variations in type III effector repertoires, pathological phenotypes and host range of Xanthomonas citri pv. citri pathotypes. Mol. Plant Pathol. 14: 483-496. DOI: 10.1111/mpp.12019

Harrison J, Studholme DJ (2014). Draft genome sequence of Xanthomonas axonopodis pathovar vasculorum NCPPB 900. FEMS Microbiol. Lett. 360: 113-116. DOI: 10.1111/1574-6968.12607

Liu Y, Long J, Shen D, Song C (2016). Xanthomonas oryzae pv. oryzae requires H-NS-family protein XrvC to regulate virulence during rice infection. FEMS Microbiol. Lett. 363: fnw067. DOI: 10.1093/femsle/fnw067

Peeters N, Carrère S, Anisimova M, Plener L, Cazalé AC, Genin S (2013). Repertoire, unified nomenclature and evolution of the type III effector gene set in the Ralstonia solanacearum species complex. BMC Genomics 14: 859. DOI: 10.1186/1471-2164-14-859

Studholme DJ, Kemen E, MacLean D, Schornack S, Aritua V, Thwaites R, Grant M, Smith J, Jones JD (2010). Genome-wide sequencing data reveals virulence factors implicated in banana Xanthomonas wilt. FEMS Microbiol. Lett. 310: 182-192. DOI: 10.1111/j.1574-6968.2010.02065.x

Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel Xanthomonas euvesicatoria type III effector proteins by a machine-learning approach. Mol. Plant Pathol. 17: 398-411. DOI: 10.1111/mpp.12288

Vicente JG, Rothwell S, Holub EB, Studholme DJ (2017). Pathogenic, phenotypic and molecular characterisation of Xanthomonas nasturtii sp. nov. and Xanthomonas floridensis sp. nov., new species of Xanthomonas associated with watercress production in Florida. Int. J. Syst. Evol. Microbiol. 67: 3645-3654. DOI: 10.1099/ijsem.0.002189

Wasukira A, Tayebwa J, Thwaites R, Paszkiewicz K, Aritua V, Kubiriba J, Smith J, Grant M, Studholme DJ (2012). Genome-wide sequencing reveals two major sub-lineages in the genetically monomorphic pathogen Xanthomonas campestris pathovar musacearum. Genes (Basel) 3: 361-377. DOI: 10.3390/genes3030361

bacteria/t3e/xopad.txt · Last modified: 2020/08/11 11:07 by rkoebnik