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--- bacteria:t3e:xopag [2020/07/09 12:25]
rkoebnik [Conservation]
+++ bacteria:t3e:xopag [2020/09/26 22:30]
nianwang_ufl.edu
@@ Line 2: / Line 2: @@
 Author: [[https://www.researchgate.net/profile/Christian_Verniere|Christian Vernière ]] & Trainees from the 2<sup>nd</sup>  EuroXanth Training School ([[https://www.researchgate.net/profile/Songul_Erken|Songül Erken]], [[https://www.researchgate.net/profile/Damla_Ertimurtas|Damla Ertimurtaş]], [[https://www.researchgate.net/profile/Jelena_Menkovic|Jelena Menković]], [[https://www.researchgate.net/profile/Andjelka_Prokic|Andjelka Prokić]])\\
-Internal reviewer: FIXME \\
+Internal reviewer: [[https://www.researchgate.net/profile/Tamas_Kovacs6|Tamás Kovács]]\\
-Expert reviewer: FIXME
+Expert reviewer: FIXME  Nian Wang
 Class: XopAG\\
 Family: XopAG\\
-Prototype: XopAG (//Xanthomonas//; strain)\\
+Prototype: XopAG (//Xanthomonas citri //pv.// citri//; strain Xac‐A<sup>w</sup>  )\\
-RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_080633702.1|WP_080633702.1]] (425 aa)\\
+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_080766906.1|WP_080766906]] (425 aa) (replacing [[https://www.ncbi.nlm.nih.gov/protein/WP_080633702.1|WP_080633702.1]])\\
 Synonym: AvrGf1\\
 D structure: Unknown
@@ Line 19: / Line 19: @@
 === (Experimental) evidence for being a T3E ===
+An active TTSS is necessary for HR produced by avrGf1 in grapefruit leaves, as it was proven by transconjugation experiments (Rybak //et al//., 2009).
 === Regulation ===
+No data available. The effector gene //xopAG// was however shown to be induced in XVM2 medium compared to NB medium in X. citri subsp. citri A<sup>W</sup>  12879 strai, a variant strain restricted to Mexicanl lime (Jalan et al., 2013).
 === Phenotypes ===
-All //xopAG//-containing strains of //X. citri// pv. //citri// induced the hypersensitive response (HR) on grapefruit (//Citrus paradisi//) and sweet orange (//C. sinensis//) but express canker symptoms on Key lime (Escalon //et al//., 2013). After infiltration of grapefruit leaves with inoculum adjusted to 5×108 cfu/mL, internal bacterial populations of Xcc-A (strain A 40) and Xcc-Aw (strain 12879) were similar through the second day, but populations of Xcc-A were significantly greater than those of Xcc-Aw after six days. The symptoms caused by the Xac-AwΔavrGf1 strain that was mutated on avrGf1 were more similar to those produced by the wild-type Xac-A strain than to those produced by the wild-type Xac-Aw strain (Rybak //et al//., 2009). So the whole pathogenicity was not restored.
+All //xopAG//-containing strains of //X. citri// pv. //citri// induced the hypersensitive response (HR) on grapefruit (//Citrus paradisi//) and sweet orange (//C. sinensis//) but express canker symptoms on Key lime (Escalon //et al//., 2013). After infiltration of grapefruit leaves with inoculum adjusted to 5×10<sup>8</sup>  cfu/mL, internal bacterial populations of Xcc-A (strain A 40) and Xcc-Aw (strain 12879) were similar through the second day, but populations of Xcc-A were significantly greater than those of Xcc-Aw after six days. The symptoms caused by the Xac-AwΔavrGf1 strain that was mutated on avrGf1 were more similar to those produced by the wild-type Xac-A strain than to those produced by the wild-type Xac-Aw strain (Rybak //et al//., 2009). So the whole pathogenicity was not restored.
 === Localization ===
@@ Line 29: / Line 31: @@
 === Enzymatic function ===
+AvrGf2 elicited rapid cell death in grapfruit leaves (Gonchez //et al//., 2015), detailed enzymatic function has not been determined yet.
 === Interaction partners ===
-The XopAG AvrGf2 effector contains a Cyp-binding site that is essential for the elicitation of HR in citrus (Gochez //et al//., 2017).
+The XopAG AvrGf2 effector contains a Cyp-binding site that is essential for the elicitation of HR in citrus (Gochez //et al//., 2017).  Yeast two-hybrid experiments showed strong interaction of AvrGf2 with grapefruit cyclophilin (GfCyp), whereas mutation of the GPLL motif in the cyclophilin-binding domain abolished the interaction.
 ===== Conservation =====
@@ Line 40: / Line 44: @@
 Yes (//e.g.//, //P. syringae.// pv. //phaseolicola// (HopG1), //P. syringae// pv. //tomato// (HopG1), //Ralstonia solanacearum//, //Acidovorax citrulli//) (Gochez //et al//., 2017).
 ===== References =====
@@ Line 46: / Line 49: @@
 Figueiredo JF, Romer P, Lahaye T, Graham JH, White FF, Jones JB (2011). //Agrobacterium//-mediated transient expression in citrus leaves: a rapid tool for gene expression and functional gene assay. Plant Cell Rep. 30: 1339-1345. DOI: [[https://doi.org/10.1007/s00299-011-1045-7|10.1007/s00299-011-1045-7]]
+<font 10.5pt/inherit;;#1c1d1e;;white>Gochez AM, Minsavage GV, Potnis N, Canteros BI, Stall RE, Jones JB (2015). A functional XopAG homologue in //Xanthomonas fuscans// pv.// aurantifolii //strain C limits host range. Plant Pathol, 64: 1207-1214. DOI:</font>  [[https://doi.org/10.1111/ppa.12361|10.1111/ppa.12361]]
 Gochez AM, Shantharaj D, Potnis N, Zhou X, Minsavage GV, White FF, Wang N, Hurlbert JC, Jones JB (2017). Molecular characterization of XopAG effector AvrGf2 from //Xanthomonas fuscans// ssp. //aurantifolii// in grapefruit. Mol. Plant Pathol. 18: 405-419. DOI: [[https://doi.org/10.1111/mpp.12408|10.1111/mpp.12408]]
+Jalan N, Kumar D, Andrade MO, Yu F, Jones JB, Graham JH, White FF, Setubal JC, Wang N (2013). Comparative genomic and transcriptome analyses of pathotypes of //Xanthomonas citri //subsp. //citri// provide insights into mechanisms of bacterial virulence and host range. BMC Genomics 14: 551. DOI: [[https://doi.org/10.1186/1471-2164-14-551|10.1186/1471-2164-14-551]]
 Rybak M, Minsavage GV, Stall RE, Jones JB (2009). Identification of //Xanthomonas citri// ssp. //citri// host specificity genes in a heterologous expression host. Mol. Plant Pathol. 10: 249-262. DOI: [[https://doi.org/10.1111/j.1364-3703.2008.00528.x|10.1111/j.1364-3703.2008.00528.x]]

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