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--- bacteria:t3e:xopag [2020/08/12 23:51]
jfpothier [References]
+++ bacteria:t3e:xopag [2021/01/25 21:59] (current)
rkoebnik
@@ Line 3: / Line 3: @@
 Author: [[https://www.researchgate.net/profile/Christian_Verniere|Christian Vernière ]] & Trainees from the 2<sup>nd</sup>  EuroXanth Training School ([[https://www.researchgate.net/profile/Songul_Erken|Songül Erken]], [[https://www.researchgate.net/profile/Damla_Ertimurtas|Damla Ertimurtaş]], [[https://www.researchgate.net/profile/Jelena_Menkovic|Jelena Menković]], [[https://www.researchgate.net/profile/Andjelka_Prokic|Andjelka Prokić]])\\
 Internal reviewer: [[https://www.researchgate.net/profile/Tamas_Kovacs6|Tamás Kovács]]\\
-Expert reviewer: FIXME
+Expert reviewer: [[https://www.researchgate.net/profile/Nian-Wang|Nian Wang]]
 Class: XopAG\\
 Family: XopAG\\
-Prototype: XopAG (//Xanthomonas citri //pv.// citri//; strain Xac‐A<sup>w</sup> )\\
+Prototype: XopAG (//Xanthomonas citri //pv.// citri//; strain Xac‐A<sup>w</sup>  )\\
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_080766906.1|WP_080766906]] (425 aa) (replacing [[https://www.ncbi.nlm.nih.gov/protein/WP_080633702.1|WP_080633702.1]])\\
 Synonym: AvrGf1\\
@@ Line 25: / Line 25: @@
 === Phenotypes ===
-All //xopAG//-containing strains of //X. citri// pv. //citri// induced the hypersensitive response (HR) on grapefruit (//Citrus paradisi//) and sweet orange (//C. sinensis//) but express canker symptoms on Key lime (Escalon //et al//., 2013). After infiltration of grapefruit leaves with inoculum adjusted to 5×108 cfu/mL, internal bacterial populations of Xcc-A (strain A 40) and Xcc-Aw (strain 12879) were similar through the second day, but populations of Xcc-A were significantly greater than those of Xcc-Aw after six days. The symptoms caused by the Xac-AwΔavrGf1 strain that was mutated on avrGf1 were more similar to those produced by the wild-type Xac-A strain than to those produced by the wild-type Xac-Aw strain (Rybak //et al//., 2009). So the whole pathogenicity was not restored.
+All //xopAG//-containing strains of //X. citri// pv. //citri// induced the hypersensitive response (HR) on grapefruit (//Citrus paradisi//) and sweet orange (//C. sinensis//) but express canker symptoms on Key lime (Escalon //et al//., 2013). After infiltration of grapefruit leaves with inoculum adjusted to 5×10<sup>8</sup>  cfu/mL, internal bacterial populations of Xcc-A (strain A 40) and Xcc-Aw (strain 12879) were similar through the second day, but populations of Xcc-A were significantly greater than those of Xcc-Aw after six days. The symptoms caused by the Xac-AwΔavrGf1 strain that was mutated on avrGf1 were more similar to those produced by the wild-type Xac-A strain than to those produced by the wild-type Xac-Aw strain (Rybak //et al//., 2009). So the whole pathogenicity was not restored.
 === Localization ===
@@ Line 31: / Line 31: @@
 === Enzymatic function ===
- AvrGf2 elicited rapid cell death in grapfruit leaves (Gonchez //et al//., 2015), detailed enzymatic function has not been determined yet.
+AvrGf2 elicited rapid cell death in grapfruit leaves (Gonchez //et al//., 2015), detailed enzymatic function has not been determined yet.
 === Interaction partners ===
-The XopAG AvrGf2 effector contains a Cyp-binding site that is essential for the elicitation of HR in citrus (Gochez //et al//., 2017).
+The XopAG AvrGf2 effector contains a Cyp-binding site that is essential for the elicitation of HR in citrus (Gochez //et al//., 2017). Yeast two-hybrid experiments showed strong interaction of AvrGf2 with grapefruit cyclophilin (GfCyp), whereas mutation of the GPLL motif in the cyclophilin-binding domain abolished the interaction.
 ===== Conservation =====

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