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bacteria:t3e:xopb [2019/05/17 15:57] 91.203.34.14 |
bacteria:t3e:xopb [2020/07/08 17:46] rkoebnik [XopB] |
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+ | ====== XopB ====== | ||
+ | |||
+ | Author: [[https:// | ||
+ | Internal reviewer: [[https:// | ||
+ | Expert reviewer: FIXME | ||
+ | |||
Class: XopB\\ | Class: XopB\\ | ||
Family: XopB\\ | Family: XopB\\ | ||
- | Prototype: XopB (// | + | Prototype: XopB (// |
- | RefSeq ID: [[https:// | + | RefSeq ID: [[https:// |
- | 3D structure: | + | 3D structure: |
+ | |||
+ | ===== Biological function ===== | ||
+ | |||
+ | === How discovered? === | ||
+ | |||
+ | XopB was discovered in a cDNA-AFLP screen (Noël //et al.//, 2001). | ||
+ | === (Experimental) evidence for being a T3E === | ||
+ | |||
+ | A chimeric protein consisting of a C-terminally truncated XopB where the last 52 residues (5 kDa) were replaced by the triple c-myc epitope (5 kDa) was secreted into culture supernatants of a strain with a constitutively active form of //hrpG// in a type III secretion-dependent manner (Noël //et al.//, 2001). XopB belongs to translocation class B (Schulze //et al.//, 2012). Mutation studies of a putative translocation motif (TrM) showed that the proline/ | ||
+ | === Regulation === | ||
+ | |||
+ | The //xopB// gene was shown to be expressed in a //hrpG//- and // | ||
+ | === Phenotypes === | ||
+ | |||
+ | A deletion of //xopB// did not affect pathogenicity or bacterial growth in plants (Noël //et al.//, 2001). Later it was found that XopB contributes to disease symptoms and bacterial growth (Schulze //et al.//, 2012; Priller //et al.//, 2016). Infection of susceptible pepper plants with a strain lacking //xopB// resulted in increased formation of salicylic acid (SA) and expression of pathogenesis-related (PR) genes (Priller //et al.//, 2016). When expressed in yeast, XopB attenuated cell proliferation (Salomon //et al.//, 2011). XopB caused a fast and confluent cell death when transiently expressed in the non-host //Nicotiana benthamiana// | ||
+ | === Localization === | ||
+ | |||
+ | XopB localizes to the Golgi apparatus and cytoplasm of the plant cell and interferes with eukaryotic vesicle trafficking (Schulze //et al.//, 2012). | ||
+ | === Enzymatic function === | ||
+ | |||
+ | Unknown. | ||
+ | |||
+ | === Interaction partners === | ||
+ | |||
+ | Unknown. | ||
+ | |||
+ | ===== Conservation ===== | ||
+ | |||
+ | === In xanthomonads === | ||
+ | |||
+ | Yes (e.g., //X. fragariae//, | ||
+ | === In other plant pathogens/ | ||
+ | |||
+ | Yes (e.g., // | ||
+ | ===== References ===== | ||
- | __Biological function__ | + | Harrison J, Studholme DJ (2014). Draft genome sequence of // |
- | <font inherit/ | + | Noël L, Thieme F, Nennstiel D, Bonas U (2001). |
- | <font inherit/ | + | |
- | <font inherit/inherit;;# | + | |
- | <font inherit/ | + | |
- | When expressed in yeast, XopB attenuated cell proliferation.< | + | |
- | <font inherit/ | + | |
- | <font inherit/ | + | |
- | <font inherit/ | + | |
- | __Conservation__ | + | Priller JPR, Reid S, Konein P, Dietrich P, Sonnewald S (2016). The // |
- | In xanthomonads: | + | Prochaska H, Thieme S, Daum S, Grau J, Schmidtke C, Hallensleben M, John P, Bacia K, Bonas U (2018). A conserved motif promotes HpaB-regulated export of type III effectors from //Xanthomonas//. Mol. Plant Pathol. 19: 2473-2487. DOI: [[https://doi.org/10.1111/mpp.12725|10.1111/mpp.12725]] |
- | In other plant pathogens/symbionts: Yes (e.g. // | + | |
- | __References__ | + | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of // |
- | - Noël L, Thieme F, Nennstiel D, Bonas U (2001). cDNA-AFLP analysis unravels a genome-wide // | + | Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // |
- | - Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of // | + | |
- | - Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // | + | |
- | - Sonnewald S, Priller JP, Schuster J, Glickmann E, Hajirezaei MR, Siebig S, Mudgett MB, Sonnewald U (2012). Regulation of cell wall-bound invertase in pepper leaves by // | + | |
- | - Harrison J, Studholme DJ (2014). Draft genome sequence of // | + | |
- | - Priller JP, Reid S, Konein P, Dietrich P, Sonnewald S (2016). The // | + | |
- | - Prochaska H, Thieme S, Daum S, Grau J, Schmidtke C, Hallensleben M, John P, Bacia K, Bonas U (2018). A conserved motif promotes HpaB-regulated export of type III effectors from // | + | |
+ | Sonnewald S, Priller JPR, Schuster J, Glickmann E, Hajirezaei MR, Siebig S, Mudgett MB, Sonnewald U (2012). Regulation of cell wall-bound invertase in pepper leaves by // | ||