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bacteria:t3e:xopb [2020/04/10 13:24] jfpothier |
bacteria:t3e:xopb [2020/07/08 17:46] rkoebnik [XopB] |
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====== XopB ====== | ====== XopB ====== | ||
- | Author: Ralf Koebnik\\ | + | Author: |
- | Reviewer: FIXME\\ | + | Internal reviewer: [[https:// |
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
Class: XopB\\ | Class: XopB\\ | ||
Family: XopB\\ | Family: XopB\\ | ||
- | Prototype: XopB (// | + | Prototype: XopB (// |
RefSeq ID: [[https:// | RefSeq ID: [[https:// | ||
3D structure: Unknown | 3D structure: Unknown | ||
Line 14: | Line 14: | ||
=== How discovered? === | === How discovered? === | ||
- | XopB was discovered in a cDNA-AFLP screen (Noël //et al.//, 2001). | ||
+ | XopB was discovered in a cDNA-AFLP screen (Noël //et al.//, 2001). | ||
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | A chimeric protein consisting of a C-terminally truncated XopB where the last 52 residues (5 kDa) were replaced by the triple c-myc epitope (5 kDa) was secreted into culture supernatants of a strain with a constitutively active form of //hrpG// in a type III secretion-dependent manner (Noël //et al.//, 2001). XopB belongs to translocation class B (Schulze //et al.//, 2012). Mutation studies of a putative translocation motif (TrM) showed that the proline/ | ||
+ | A chimeric protein consisting of a C-terminally truncated XopB where the last 52 residues (5 kDa) were replaced by the triple c-myc epitope (5 kDa) was secreted into culture supernatants of a strain with a constitutively active form of //hrpG// in a type III secretion-dependent manner (Noël //et al.//, 2001). XopB belongs to translocation class B (Schulze //et al.//, 2012). Mutation studies of a putative translocation motif (TrM) showed that the proline/ | ||
=== Regulation === | === Regulation === | ||
- | The //xopB// gene was shown to be expressed in a //hrpG//- and // | ||
+ | The //xopB// gene was shown to be expressed in a //hrpG//- and // | ||
=== Phenotypes === | === Phenotypes === | ||
- | A deletion of //xopB// did not affect pathogenicity or bacterial growth in plants (Noël //et al.//, 2001). Later it was found that XopB contributes to disease symptoms and bacterial growth (Schulze //et al.//, 2012; Priller //et al.//, 2016). Infection of susceptible pepper plants with a strain lacking //xopB// resulted in increased formation of salicylic acid (SA) and expression of pathogenesis-related (PR) genes (Priller //et al.//, 2016). | ||
- | When expressed in yeast, XopB attenuated cell proliferation (Salomon //et al.//, 2011). XopB caused a fast and confluent cell death when transiently expressed in the non-host //Nicotiana benthamiana// | + | A deletion of //xopB// did not affect pathogenicity or bacterial growth in plants (Noël //et al.//, 2001). Later it was found that XopB contributes to disease symptoms and bacterial growth (Schulze //et al.//, 2012; Priller //et al.//, 2016). Infection of susceptible pepper plants with a strain lacking //xopB// resulted in increased formation of salicylic acid (SA) and expression of pathogenesis-related (PR) genes (Priller //et al.//, 2016). |
+ | === Localization === | ||
- | === Localisation === | ||
XopB localizes to the Golgi apparatus and cytoplasm of the plant cell and interferes with eukaryotic vesicle trafficking (Schulze //et al.//, 2012). | XopB localizes to the Golgi apparatus and cytoplasm of the plant cell and interferes with eukaryotic vesicle trafficking (Schulze //et al.//, 2012). | ||
- | |||
=== Enzymatic function === | === Enzymatic function === | ||
+ | |||
Unknown. | Unknown. | ||
=== Interaction partners === | === Interaction partners === | ||
+ | |||
Unknown. | Unknown. | ||
- | ===== Conservation ===== | + | ===== Conservation ===== |
- | === In xanthomonads === | + | === In xanthomonads === |
- | Yes (e.g., //X. fragariae//, | + | |
+ | Yes (e.g., //X. fragariae//, | ||
=== In other plant pathogens/ | === In other plant pathogens/ | ||
- | Yes (e.g., // | ||
- | ===== References ===== | + | Yes (e.g., // |
+ | ===== References ===== | ||
- | Harrison J, Studholme DJ (2014). Draft genome sequence of // | + | Harrison J, Studholme DJ (2014). Draft genome sequence of // |
- | Noël L, Thieme F, Nennstiel D, Bonas U (2001). cDNA-AFLP analysis unravels a genome-wide // | + | Noël L, Thieme F, Nennstiel D, Bonas U (2001). cDNA-AFLP analysis unravels a genome-wide // |
- | Priller | + | Priller |
- | Prochaska H, Thieme S, Daum S, Grau J, Schmidtke C, Hallensleben M, John P, Bacia K, Bonas U (2018). A conserved motif promotes HpaB-regulated export of type III effectors from // | + | Prochaska H, Thieme S, Daum S, Grau J, Schmidtke C, Hallensleben M, John P, Bacia K, Bonas U (2018). A conserved motif promotes HpaB-regulated export of type III effectors from // |
- | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of // | + | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of // |
- | Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // | + | Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // |
- | Sonnewald S, Priller | + | Sonnewald S, Priller |