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--- bacteria:t3e:xope2 [2020/07/08 18:34]
rkoebnik [XopE2]
+++ bacteria:t3e:xope2 [2020/07/15 13:16] (current)
rkoebnik [Biological function]
@@ Line 10: / Line 10: @@
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011347479.1|WP_011347479.1]] (358 aa)\\
 Synonym: AvrXacE3 (//Xanthomonas citri// pv. //citri//); AvrXccE1 (//Xanthomonas campestris// pv. //campestris//)\\
-D structure: Myristoylation motif at their extreme N terminus.
+D structure: Myristoylation motif at the extreme N terminus (Thieme //et al.//, 2007).
 ===== Biological function =====
@@ Line 25: / Line 25: @@
 === Phenotypes ===
-XopE2 shows an avirulence activity in //Solanum pseudocapsicum// (Thieme //et al.//, 2007) and //Agrobacterium// mediated transient expression of XopE2 shows avirulence activity in the ornamental plant //S. pseudocapsicum// (Lin //et al//., 2011). XopE2 proteins were shown to be capable of suppressing the hypersensitive response (HR) of //Nicotiana// spp. induced by HopPsyA of //P. syringae //pv. //syringae// 61 and the reaction occurs within the plant cells after their delivery by TTSS (Lin //et al//., 2011). XopE2 inhibits growth of yeast cells in the presence of sodium chloride and caffeine (Salomon //et al//., 2011), and expression of XopE2 in yeast affects the yeast cell wall and the endoplasmic reticulum stress response (Bosis //et al//., 2011). XopE2 appears to promote wall-bound invertase activity in pepprt leaves (Sonnewald //et al.//, 2011). XopE2 mutants grow to equivalent titers as wild type //X. euvesicatoria// in tomato leaves indicating that is not required for bacterial multiplication in planta. XopE2 together with XopE1 and XopO may function redundantly to inhibit //X//. //euvesicatoria// induced chlorosis in tomato leaves (Dubrow //et al//., 2018). XopE2 inhibits the activation of a PTI-inducible promoter by the bacterial peptide elf18 in //Arabidopsis //protoplasts and by flg22 in tomato protoplasts. This effector inhibits flg22-induced callose deposition in planta and enhanced disease symptoms caused by attenuated //Pseudomonas syringae// bacteria (Popov //et al//., 2016).
+  * XopE2 shows an avirulence activity in //Solanum pseudocapsicum//  (Thieme //et al.//, 2007).
+  * //Agrobacterium//  mediated transient expression of XopE2 shows avirulence activity in the ornamental plant //S. pseudocapsicum//  (Lin //et al//., 2011).
+  * XopE2 proteins were shown to be capable of suppressing the hypersensitive response (HR) of //Nicotiana//  spp. induced by HopPsyA of //P. syringae //pv. //syringae//  61 and the reaction occurs within the plant cells after their delivery by TTSS (Lin //et al//., 2011).
+  * XopE2 inhibits growth of yeast cells in the presence of sodium chloride and caffeine (Salomon //et al//., 2011).
+  * Expression of XopE2 in yeast affects the yeast cell wall and the endoplasmic reticulum stress response (Bosis //et al//., 2011).
+  * XopE2 appears to promote wall-bound invertase activity in pepprt leaves (Sonnewald //et al.//, 2011).
+  * XopE2 mutants grow to equivalent titers as wild type //X. euvesicatoria//  in tomato leaves indicating that is not required for bacterial multiplication in planta. XopE2 together with XopE1 and XopO may function redundantly to inhibit //X//. //euvesicatoria//  induced chlorosis in tomato leaves (Dubrow //et al//., 2018).
+  * XopE2 inhibits the activation of a PTI-inducible promoter by the bacterial peptide elf18 in //Arabidopsis //protoplasts and by flg22 in tomato protoplasts. This effector inhibits flg22-induced callose deposition //in planta //and enhanced disease symptoms caused by attenuated //Pseudomonas syringae//  bacteria (Popov //et al//., 2016).
 === Localization ===
-XopE2 fused to gfp in a binary vector under control of the Cauliflower mosaic virus 35S promoter expressed in //Nicotiana benthamiana// leaves, using //Agrobacterium//-mediated gene transfer, allowed to localize XopE2::GFP confined to the periphery of the cells and not detectable in the nucleus or in the cytoplasm. Localization of the XopE2::GFP to the plasma membrane of //N. benthamiana //mesophyll cells could be confirmed by immunocytochemistry (Thieme //et al//., 2007).
+XopE2 fused to GFP in a binary vector under control of the Cauliflower mosaic virus 35S promoter expressed in //Nicotiana benthamiana//  leaves, using //Agrobacterium//-mediated gene transfer, allowed to localize XopE2::GFP confined to the periphery of the cells and not detectable in the nucleus or in the cytoplasm. Localization of the XopE2::GFP to the plasma membrane of //N. benthamiana //mesophyll cells could be confirmed by immunocytochemistry (Thieme //et al//., 2007).
 === Enzymatic function ===
 XopE2 belongs to the HopX effector family, which are part of the transglutaminase superfamily (Nimchuk //et al//., 2007).
 === Interaction partners ===
 XopE2 was found to physically interact with tomato 14-3-3 (TFT) proteins. XopE2 is phosphorylated at multiple residues //in planta //for maximal binding to TFT10 (Dubrow //et al//., 2018).
 ===== Conservation =====

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