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bacteria:t3e:xopj1

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bacteria:t3e:xopj1 [2020/09/21 16:09]
fboernke 		bacteria:t3e:xopj1 [2020/09/21 16:23]
fboernke
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 Author: [[https://www.researchgate.net/profile/Jens_Boch|Jens Boch]]\\ Author: [[https://www.researchgate.net/profile/Jens_Boch|Jens Boch]]\\
 Internal reviewer: [[https://www.researchgate.net/profile/Joana_Costa12|Joana Costa]]\\ Internal reviewer: [[https://www.researchgate.net/profile/Joana_Costa12|Joana Costa]]\\
-Expert reviewer: FIXME+Expert reviewer: Frederik Börnke
  
 Class: XopJ\\ Class: XopJ\\
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 === Localization === === Localization ===
  
-Following type III translocation, XopJ localizes to the plant plasma membrane via N-terminal myristoylation by the host cell (Thieme //et al//., 2007; Bartetzko //et al//., 2009). A wildtype XopJ-GFP fusion (not a mutant in the catalytic triad) also localizes to vesicle-like structures that colocalize with Golgi-marker proteins.+XopJ carries a predicted N-myristoylation motif on a glycine residue at position two of the polypeptide. Following type III translocation, XopJ localizes to the plant plasma membrane via N-terminal myristoylation by the host cell (Thieme //et al//., 2007; Bartetzko //et al//., 2009). Mutation of the glycine residue at postion two into alanine (G2A) renders the protein soluble. A wildtype XopJ-GFP fusion (not a mutant in the catalytic triad) also localizes to vesicle-like structures that colocalize with Golgi-marker proteins.
 === Enzymatic function === === Enzymatic function ===
  
-XopJ belongs to the group of YopJ-family effectors and is a member of the YopJ/AvrRxv family of SUMO peptidases and acetyltransferases. These are characterized as C55 cysteine proteases, ubiquitin-like proteases (deSUMOylation), or acetyltransferases. Such enzymes share a characteristic catalytic triad consisting of the amino acids histidine, glutamic or aspartic acid, and cysteine. XopJ has Cys protease activity //in vitro// and //in vivo// (Üstün & Börnke, 2015).+XopJ belongs to the group of YopJ-family effectors and is a member of the YopJ/AvrRxv family of SUMO peptidases and acetyltransferases. These are characterized as C55 cysteine proteases, ubiquitin-like proteases (deSUMOylation), or acetyltransferases. Such enzymes share a characteristic catalytic triad consisting of the amino acids histidine, glutamic or aspartic acid, and cysteine. XopJ has Cys protease activity //in vitro// and //in vivo//, but seems to lack acetyltransferase activity under standard assay conditions (Üstün & Börnke, 2015).
 === Interaction partners === === Interaction partners ===
  
-19S RP subunit RPT6 (RP ATPase 6) of the 26S proteasome (Üstün & Börnke, 2015). The interaction is dependent on the Walker A motif (ATP binding) of RPT6. +19S RP subunit RPT6 (RP ATPase 6) of the 26S proteasome (Üstün & Börnke, 2015). The interaction is dependent on the Walker A motif (ATP binding) of RPT6. The interaction between the two proteins has been shown by yeast two-hybrid assays, //in vivo// and //in vitro// pull-down, as well as by bimolecular fluorescence assays //in planta// (Üstün et al., 2013).
 ===== Conservation ===== ===== Conservation =====
  
bacteria/t3e/xopj1.txt · Last modified: 2020/09/21 18:15 by rkoebnik

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