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--- bacteria:t3e:xopj1 [2020/09/21 16:18]
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+++ bacteria:t3e:xopj1 [2020/09/21 16:23]
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 === Enzymatic function ===
-XopJ belongs to the group of YopJ-family effectors and is a member of the YopJ/AvrRxv family of SUMO peptidases and acetyltransferases. These are characterized as C55 cysteine proteases, ubiquitin-like proteases (deSUMOylation), or acetyltransferases. Such enzymes share a characteristic catalytic triad consisting of the amino acids histidine, glutamic or aspartic acid, and cysteine. XopJ has Cys protease activity //in vitro// and //in vivo// (Üstün & Börnke, 2015).
+XopJ belongs to the group of YopJ-family effectors and is a member of the YopJ/AvrRxv family of SUMO peptidases and acetyltransferases. These are characterized as C55 cysteine proteases, ubiquitin-like proteases (deSUMOylation), or acetyltransferases. Such enzymes share a characteristic catalytic triad consisting of the amino acids histidine, glutamic or aspartic acid, and cysteine. XopJ has Cys protease activity //in vitro// and //in vivo//, but seems to lack acetyltransferase activity under standard assay conditions (Üstün & Börnke, 2015).
 === Interaction partners ===
-S RP subunit RPT6 (RP ATPase 6) of the 26S proteasome (Üstün & Börnke, 2015). The interaction is dependent on the Walker A motif (ATP binding) of RPT6.
+S RP subunit RPT6 (RP ATPase 6) of the 26S proteasome (Üstün & Börnke, 2015). The interaction is dependent on the Walker A motif (ATP binding) of RPT6. The interaction between the two proteins has been shown by yeast two-hybrid assays, //in vivo// and //in vitro// pull-down, as well as by bimolecular fluorescence assays //in planta// (Üstün et al., 2013).
 ===== Conservation =====

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