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bacteria:t3e:xopj2 [2020/07/08 18:55] rkoebnik |
bacteria:t3e:xopj2 [2020/08/11 14:43] (current) rkoebnik |
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Author: [[https:// | Author: [[https:// | ||
- | Internal reviewer: | + | Internal reviewer: |
Expert reviewer: FIXME | Expert reviewer: FIXME | ||
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* Resistance in Pi-0 was found to be caused by a recessive mutation predicted to inactivate a carboxylesterase known to hydrolyze lysophospholipids and acylated proteins in eukaryotes. Transgenic Pi-0 plants expressing the wild-type allele from the //A. thaliana// | * Resistance in Pi-0 was found to be caused by a recessive mutation predicted to inactivate a carboxylesterase known to hydrolyze lysophospholipids and acylated proteins in eukaryotes. Transgenic Pi-0 plants expressing the wild-type allele from the //A. thaliana// | ||
* It was later shown that Pi-0 leaves infected with // | * It was later shown that Pi-0 leaves infected with // | ||
- | * Transgenic // | + | * Transgenic // |
* Phylogenomics revealed that a host-range expansion of //X. euvesicatoria// | * Phylogenomics revealed that a host-range expansion of //X. euvesicatoria// | ||
* Later, AvrBsT was found to contribute to fitness of // | * Later, AvrBsT was found to contribute to fitness of // | ||
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=== Enzymatic function === | === Enzymatic function === | ||
- | AvrBsT belongs to the YopJ family, members of which were shown to act as cysteine proteases, which contain | + | AvrBsT belongs to the YopJ family, members of which were shown to act as cysteine proteases |
=== Interaction partners === | === Interaction partners === | ||
* Yeast two-hybrid based analyses identified a putative regulator of sugar metabolism, SNF1-related kinase 1 (SnRK1), as an interactor of AvrBsT (Szczesny //et al//., 2010). Gene silencing experiments revealed that SnRK1 is required for the induction of the AvrBs1-specific HR, which is suppressed by AvrBsT (Szczesny //et al//., 2010). Thus, SnRK1 may be involved in the AvrBsT-mediated suppression of the AvrBs1-specific HR (Szczesny //et al//., 2010). | * Yeast two-hybrid based analyses identified a putative regulator of sugar metabolism, SNF1-related kinase 1 (SnRK1), as an interactor of AvrBsT (Szczesny //et al//., 2010). Gene silencing experiments revealed that SnRK1 is required for the induction of the AvrBs1-specific HR, which is suppressed by AvrBsT (Szczesny //et al//., 2010). Thus, SnRK1 may be involved in the AvrBsT-mediated suppression of the AvrBs1-specific HR (Szczesny //et al//., 2010). | ||
- | * Later, the pepper SGT1 (for suppressor of the G2 allele of //skp1//) and PIK1 (for receptor-like cytoplasmic kinase1) were identified as a host interactor | + | * Later, the pepper SGT1 (for suppressor of the G2 allele of //skp1//) and PIK1 (for receptor-like cytoplasmic kinase1) were identified as host interactors |
* Using a yeast two-hybrid screen, the pepper CaHSP70a was identified as another AvrBsT-interacting protein. Bimolecular fluorescence complementation and co-immunoprecipitation assays confirmed the specific interaction between CaHSP70a and AvrBsT //in planta// | * Using a yeast two-hybrid screen, the pepper CaHSP70a was identified as another AvrBsT-interacting protein. Bimolecular fluorescence complementation and co-immunoprecipitation assays confirmed the specific interaction between CaHSP70a and AvrBsT //in planta// | ||
* Using a yeast two-hybrid screen, the pepper aldehyde dehydrogenase 1 (CaALDH1) was identified as another AvrBsT-interacting protein. Bimolecular fluorescence complementation and co-immunoprecipitation assays confirmed the interaction between CaALDH1 and AvrBsT //in planta// | * Using a yeast two-hybrid screen, the pepper aldehyde dehydrogenase 1 (CaALDH1) was identified as another AvrBsT-interacting protein. Bimolecular fluorescence complementation and co-immunoprecipitation assays confirmed the interaction between CaALDH1 and AvrBsT //in planta// | ||
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Yes (// | Yes (// | ||
- | ===== Conservation | + | ===== |
- | + | ||
- | === In xanthomonads === | + | |
- | + | ||
- | Yes (//X. euvesicatoria//, | + | |
- | + | ||
- | === In other plant pathogens/ | + | |
- | + | ||
- | Yes (// | + | |
===== References ===== | ===== References ===== | ||
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Kim NH, Hwang BK (2015b). Pepper aldehyde dehydrogenase CaALDH1 interacts with // | Kim NH, Hwang BK (2015b). Pepper aldehyde dehydrogenase CaALDH1 interacts with // | ||
- | Kim NH, Kim DS, Chung EH, Hwang BK (2014). Pepper suppressor of the G2 allele of // | + | Kim NH, Kim DS, Chung EH, Hwang BK (2014). Pepper suppressor of the G2 allele of // |
Kim NH, Kim BS, Hwang BK (2013). Pepper arginine decarboxylase is required for polyamine and γ-aminobutyric acid signaling in cell death and defense response. Plant Physiol. 162: 2067-2083. DOI: [[https:// | Kim NH, Kim BS, Hwang BK (2013). Pepper arginine decarboxylase is required for polyamine and γ-aminobutyric acid signaling in cell death and defense response. Plant Physiol. 162: 2067-2083. DOI: [[https:// | ||
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===== Further reading ===== | ===== Further reading ===== | ||
+ | |||
+ | Han SW, Hwang BK (2017). Molecular functions of // | ||
Timilsina S, Abrahamian P, Potnis N, Minsavage GV, White FF, Staskawicz BJ, Jones JB, Vallad GE, Goss EM (2016). Analysis of sequenced genomes of Xanthomonas perforans identifies candidate targets for resistance breeding in tomato. Phytopathology 106: 1097-1104. DOI: [[https:// | Timilsina S, Abrahamian P, Potnis N, Minsavage GV, White FF, Staskawicz BJ, Jones JB, Vallad GE, Goss EM (2016). Analysis of sequenced genomes of Xanthomonas perforans identifies candidate targets for resistance breeding in tomato. Phytopathology 106: 1097-1104. DOI: [[https:// | ||