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bacteria:t3e:xopj3

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bacteria:t3e:xopj3 [2020/11/27 10:02]
doron.teper 		bacteria:t3e:xopj3 [2020/11/27 10:18] (current)
doron.teper
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-[[https://www.researchgate.net/profile/Doron_Teper|Author: Doron Teper]]\\ +====== XopJ3 ======
-Reviewer:+
  
-<font 12.0pt/inherit;;inherit;;inherit>Class: XopJ\\+Author: [[https://www.researchgate.net/profile/Doron_Teper|Doron Teper]]\\ 
 +Internal reviewer: 
 + 
 +Class: XopJ\\
 Family: XopJ3\\ Family: XopJ3\\
-Prototype: XCV0471 ( CKGE_TMP_i Xanthomonas CKGE_TMP_i   CKGE_TMP_i euvesicatoria CKGE_TMP_i )\\ +Prototype: AvrRxv/XCV0471 (//Xanthomonas euvesicatoria// 85-10)\\ 
-RefSeq ID:</font> [[http://www.ncbi.nlm.nih.gov/protein/CAJ22102|CAJ22102]]\\ +RefSeq ID: [[http://www.ncbi.nlm.nih.gov/protein/CAJ22102|CAJ22102]]\\ 
-<font 12.0pt/inherit;;inherit;;inherit>Synonym: AvrRxv\\ +3D structure: Unknown
-3D structure: Unknown</font> +
- +
-<font 8.0pt/inherit;;inherit;;inherit>Top of Form</font> +
- +
-<font 8.0pt/inherit;;inherit;;inherit>Bottom of Form</font> +
- +
-<font 18.0pt/inherit;;inherit;;inherit>**Biological function</font> ** +
- +
-<font 12.0pt/inherit;;inherit;;inherit>**How discovered?</font> ** +
- +
-<font 12.0pt/inherit;;inherit;;inherit>AvrRxv/XopJ3 of  CKGE_TMP_i X. euvesicatoria  CKGE_TMP_i was identified to promote HR in non-host bean plants (Whalen et al. 1988).</font> +
- +
-<font 12.0pt/inherit;;inherit;;inherit>**(Experimental) evidence for being a T3E</font> ** +
- +
-<font 12.0pt/inherit;;inherit;;inherit>Tn5 insertions in multiple  CKGE_TMP_i hrp CKGE_TMP_i  genes in XcvT 75-3 eliminated AvrRxv/XopJ3-mediated HR in tomato line Hawaii 7998 (Ciesiolka et al. 1999). Disruption of the type III secretion system in  CKGE_TMP_i Xcv CKGE_TMP_i  race T3 abolished secretion of AvrRxv into culture media (Bonshtien et al. 2004).</font> +
- +
-<font 12.0pt/inherit;;inherit;;inherit>**Regulation</font> ** +
- +
-<font 12.0pt/inherit;;inherit;;inherit>CKGE_TMP_i avrRxv</font> //<font 12.0pt/inherit;;inherit;;inherit>is constitutively expressed in  CKGE_TMP_i Xanthomonas CKGE_TMP_i  tomato strain XcvT 92-14 (Ciesiolka et al. 1999). The expression of  CKGE_TMP_i avrRxv CKGE_TMP_i  was similar in NYB and XVM2 and was not dependent on  CKGE_TMP_i hrpG CKGE_TMP_i  or  CKGE_TMP_i hrpX CKGE_TMP_i  (Ciesiolka et al 1999).  CKGE_TMP_i avrRxv CKGE_TMP_i  has a perfect  PIP-box  starting  at  bp  465  with  the  sequence  TTCGC-N15-TTCGC (Ciesiolka et al 1999).</font> +
- +
-<font 12.0pt/inherit;;inherit;;inherit>**Phenotypes</font> **+
  
-<font 12.0pt/inherit;;inherit;;inherit>CKGE_TMP_i avrRxv</font> //<font 12.0pt/inherit;;inherit;;inherit>induce hypersensitive response in tomato line Hawaii 7998 (Whalen et al. 1993)(</font>  <font 12.0pt/inherit;;inherit;;inherit>Bonshtien et al. 2004), bean (Whalen et al. 1988) and  CKGE_TMP_i Nicotiana benthamiana  CKGE_TMP_i (Albers et al. 2019) CKGE_TMP_i .  CKGE_TMP_i Inactivation of  CKGE_TMP_i arvRxv CKGE_TMP_i  ( CKGE_TMP_i avrRxv CKGE_TMP_i ::</font>  <font 12.0pt/inherit;;inherit;;inherit>Ω ) in XcvT 75-3 eliminated HR induction and promoted growth in tomato line Hawaii 7998 (Whalen et al. 1993).  CKGE_TMP_i Xcv CKGE_TMP_i  race T3 ( CKGE_TMP_i X. perforans CKGE_TMP_i ) introduced with  CKGE_TMP_i avrRxv CKGE_TMP_i  induced resistance response in tomato line Hawaii 7998 which was characterized by HR, reduced bacterial growth  CKGE_TMP_i in planta CKGE_TMP_i  and significant alteration in host transcriptional profile (Bonshtien et al. 2004).</font>+===== Biological function =====
  
-<font 12.0pt/inherit;;inherit;;inherit>**Localization</font> **+=== How discovered? ===
  
-<font 12.0pt/inherit;;inherit;;inherit>AvrRxv is localized predominantly to the cytoplasm and is possibly associated with the plasma and nuclear membranes (Bonshtien et al. 2004).</font>+AvrRxv/XopJ3 of //X. euvesicatoria //was identified to promote HR in non-host bean plants (Whalen et al. 1988). 
 +=== (Experimental) evidence for being a T3E ===
  
-<font 12.0pt/inherit;;inherit;;inherit>**Enzymatic function</font> **+Tn5 insertions in multiple hrp genes in //XcvT //75-3 eliminated AvrRxv/XopJ3-mediated HR in tomato line Hawaii 7998 (Ciesiolka et al. 1998). Disruption of the type III secretion system in //Xcv //race T3 abolished secretion of AvrRxv into culture media (Bonshtien et al. 2004). 
 +=== Regulation ===
  
-<font 12.0pt/inherit;;inherit;;inherit>AvrRxv/XopJ3 belongs to the group of YopJ-family effectors. YopJ-family effectors share a conserved catalytic triad that is identical to that of the C55 family of cysteine proteases (Ma and Ma, 2016). Mutations in the AvrRxv protease catalytic core impaired elicitation of HR and inhibition of bacterial growth in tomato</font> <font 12.0pt/inherit;;inherit;;inherit>line Hawaii 7998 (Bonshtien et al. 2004).</font>+//avrRxv //is constitutively expressed in Xanthomonas tomato strain //XcvT //92-14 (Ciesiolka et al. 1998). The expression of avrRxv was similar in NYB and XVM2 and was not dependent on //hrpG //or //hrpX // (Ciesiolka et al 1998). //avrRxv //has a perfect PIP-box starting at bp 465 with the sequence TTCGC-N15-TTCGC (Ciesiolka et al 1998). 
 +=== Phenotypes ===
  
-<font 12.0pt/inherit;;inherit;;inherit>**Interaction partners</font> **+AvrRxv induce hypersensitive response in tomato line Hawaii 7998 (Whalen et al. 1993)( Bonshtien et al. 2004), bean (Whalen et al. 1988) and //Nicotiana benthamiana// (Albers et al. 2019). Inactivation of //arvRxv // (//avrRxv//:: Ω ) in //XcvT //75-3 eliminated HR induction and promoted growth in tomato line Hawaii 7998 (Whalen et al. 1993). //Xcv //race T3 (//X. perforans//) introduced with //avrRxv //induced resistance response in tomato line Hawaii 7998 which was characterized by HR, reduced bacterial growth in planta and significant alteration in host transcriptional profile (Bonshtien et al. 2004). 
 +=== Localization ===
  
-<font 12.0pt/inherit;;inherit;;inherit>AvrRxv interacts with tomato 14-3-3 protein (Whalen et al. 2008).</font>+AvrRxv is localized predominantly to the cytoplasm and is possibly associated with the plasma and nuclear membranes (Bonshtien et al. 2004).
  
-<font 8.0pt/inherit;;inherit;;inherit>Top of Form</font>+=== Enzymatic function ===
  
-<font 8.0pt/inherit;;inherit;;inherit>Bottom of Form</font>+AvrRxv/XopJ3 belongs to the group of YopJ-family effectors. YopJ-family effectors share a conserved catalytic triad that is identical to that of the C55 family of cysteine proteases (Ma and Ma, 2016). Mutations in the AvrRxv protease catalytic core impaired elicitation of HR and inhibition of bacterial growth in tomato line Hawaii 7998 (Bonshtien et al. 2004).
  
-<font 18.0pt/inherit;;inherit;;inherit>**Conservation</font> **+=== Interaction partners ===
  
-<font 12.0pt/inherit;;inherit;;inherit>**In xanthomonads</font> **+AvrRxv interacts with tomato 14-3-3 protein (Whalen et al. 2008).
  
-<font 12.0pt/inherit;;inherit;;inherit>Yes ( CKGE_TMP_i X. euvesicatoria, X. hortorum , X. nasturtii, X. axonopodis CKGE_TMP_i )</font>+<font 18.0pt/inherit;;inherit;;inherit>**Conservation**</font>
  
-<font 12.0pt/inherit;;inherit;;inherit>**In other plant pathogens/symbionts</font> **+=== In xanthomonads ===
  
-<font 11pt/inherit;;inherit;;inherit>Yes (//Pseudomonas//  CKGE_TMP_i syringe CKGE_TMP_i  spp. (HopZ-family), //Ralstonia solanacearum// (PopP1), //Acidovorax// //citrulli//, //Bradyrhizobium// sp//.//, //Mesorhizobium// sp., //Sinorhizobium fredii//)</font>+YES (//X. euvesicatoria//, //X. hortorum// , //X. nasturtii//, //X. axonopodis//) 
 +=== In other plant pathogens/symbionts ===
  
 +YES (//Pseudomonas syringae// spp. (HopZ-family), //Ralstonia solanacearum// (PopP1), //Acidovorax citrulli//, //Bradyrhizobium //sp., //Mesorhizobium //sp., //Sinorhizobium //sp.)
 ===== References ===== ===== References =====
  
bacteria/t3e/xopj3.1606467741.txt.gz · Last modified: 2020/11/27 10:02 by doron.teper

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