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bacteria:t3e:xopj5 [2020/08/09 18:05]
rkoebnik 		bacteria:t3e:xopj5 [2020/08/09 18:11] (current)
rkoebnik [Biological function]
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-T3E-defective mutant was not capable of secreting the effector protein when carrying the plasmid-borne gene encoding for AvrXccB. +AvrXccB, fused to a HA tag, was shown to be secreted into culture supernatants by Western blot analysis using an anti‐haemagglutinin (HA) antibody. In contrast, a T3E-defective mutant was not capable of secreting this effector (Liu //et al//., 2017).
 === Regulation === === Regulation ===
  
-The //xopE1// <sub>Xcc8004</sub> gene (//avrXccB//) contains a PIP box and was shown to be controlled by //hrpG// and //hrpX// (Jiang et al., 2009). (Rongqi //et al//., 2006; Jiang //et al//., 2009).+The //xopE1// <sub>Xcc8004</sub> gene (//avrXccB//) contains a PIP box and was shown to be controlled by //hrpG// and //hrpX// (Rongqi //et al//., 2006; Jiang et al., 2009).
 === Phenotypes === === Phenotypes ===
  
   * AvrXccB (XopJ5<sub>Xcc8004</sub>) is not required for full virulence and growth of //Xcc//  in the host plant Chinese radish (Jiang //et al.//, 2009).   * AvrXccB (XopJ5<sub>Xcc8004</sub>) is not required for full virulence and growth of //Xcc//  in the host plant Chinese radish (Jiang //et al.//, 2009).
-  * +  * XopJ5 was found to be associated with variations in disease symptoms when testing a set of 45 //X. campestris //pv. //campestris //strains on two //Arabidopsis//  natural accessions (Guy //et al.//, 2013).
- +
-XopJ5 was found to be associated with variations in disease symptoms when testing a set of 45 //X. campestris //pv. //campestris //strains on two Arabidopsis natural accessions (Guy //et al.//, 2013). +
   * Experimental evidence using heterologous expression of //avrXccB//  suggests that this protein is involved in the suppression of plant immunity. Immunity suppression in //Arabidopsis//  involved multiple mechanisms: ectopic expression of //avrXccB//  reduced flg22-induced callose deposition and ROS production, while //avrXccB//  expression in //Arabidopsis//  protoplasts inhibited the promoter activity of resistance gene //NHO1//. Heterologous expression of //avrXccB//  led to an increase in the in-planta population of //Xcc//  B186 and //Pst//  DC300 in //Arabidopsis//. Conversely, //avrXccB//  mutants were compromised in their ability to inhibit MAMP-induced immunity (callose deposition, ROS production and defence-related genes in //Arabidopsis//  protoplasts) (Liu //et al//., 2017).   * Experimental evidence using heterologous expression of //avrXccB//  suggests that this protein is involved in the suppression of plant immunity. Immunity suppression in //Arabidopsis//  involved multiple mechanisms: ectopic expression of //avrXccB//  reduced flg22-induced callose deposition and ROS production, while //avrXccB//  expression in //Arabidopsis//  protoplasts inhibited the promoter activity of resistance gene //NHO1//. Heterologous expression of //avrXccB//  led to an increase in the in-planta population of //Xcc//  B186 and //Pst//  DC300 in //Arabidopsis//. Conversely, //avrXccB//  mutants were compromised in their ability to inhibit MAMP-induced immunity (callose deposition, ROS production and defence-related genes in //Arabidopsis//  protoplasts) (Liu //et al//., 2017).
  
bacteria/t3e/xopj5.1596989132.txt.gz · Last modified: 2020/08/09 18:05 by rkoebnik

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